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Data describe designed environmental DNA (eDNA) experiments for the estimation of eDNA shedding rates for the spectaclecase (Cumberlandia monodonta), mucket (Actinonaias ligamentina), and fatmucket (Lampsilis siliquoidea) from water samples collected in the laboratory under differing laboratory conditions. Shedding rates were tested under variable mussel biomass, diet, and temperature. Parameters described include descriptions of laboratory collected water samples that were tested to estimate concentration of spectaclecase, mucket or fatmucket DNA qPCR results, and associated quality assurance measurements.
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The Great Plains Landscape Conservation Cooperative (GPLCC, https://www.fws.gov/science/catalog) is a partnership that provides applied science and decision support tools to assist natural resource managers conserve plants, fish and wildlife in the mid- and short-grass prairie of the southern Great Plains. It is part of a national network of public-private partnerships — known as Landscape Conservation Cooperatives (LCCs, http://www.fws.gov/science/shc/lcc.html) — that work collaboratively across jurisdictions and political boundaries to leverage resources and share science capacity. The Great Plains LCC identifies science priorities for the region and helps foster science that addresses these priorities to support...
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The Great Plains Landscape Conservation Cooperative (GPLCC, https://www.fws.gov/science/catalog) is a partnership that provides applied science and decision support tools to assist natural resource managers conserve plants, fish and wildlife in the mid- and short-grass prairie of the southern Great Plains. It is part of a national network of public-private partnerships — known as Landscape Conservation Cooperatives (LCCs, http://www.fws.gov/science/shc/lcc.html) — that work collaboratively across jurisdictions and political boundaries to leverage resources and share science capacity. The Great Plains LCC identifies science priorities for the region and helps foster science that addresses these priorities to support...
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Data describe designed environmental DNA (eDNA) experiments for the estimation of eDNA shedding rates for mucket (Actinonaias ligamentina) and fatmucket (Lampsilis siliquoidea) from water samples collected in the laboratory under differing food avilability scenarios. Parameters described include descriptions of laboratory collected water samples that were tested to estimate concentration of spectaclecase, mucket or fatmucket DNA qPCR results, and associated quality assurance measurements.
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This data release includes metadata and tabular data that documents lab and field trials testing the efficacy of carbon dioxide (CO2) gas diffused into water to manage invasive fish in anchialine pools. The data release also includes information documenting sensitivity, specificity, and accuracy of quantitative polymerase chain reaction (qPCR) assays to detect environmental DNA (eDNA) from tilapia (Oreochromis mosambicus), western mosquitofish (Gambusia affinis) and guppies (Poecilia reticulata). In total there are 11 datasets, 9 describing lab trials and 2 describing field trials. Lab data include 1) initial water conditions during pilot study, 2) behavioral response of fish to CO2 during pilot study, 3) survival...
This data contains environmental DNA (eDNA) sample assay results that were collected from water samples taken from a tank housing Asian carp and placed either on ice or at room temperature. At both treatment temperatures, water samples were left untreated or were treated with an ethanol and sodium acetate solution (EtOH-NaAc). This was done to evaluate an ethanol and sodium acetate solution to maintain the integrity of the DNA samples for the time between collection and lab testing. Every day for 6 days following collection, a subset of the samples was removed from each treatment and DNA was extracted and nuclear and mitochondrial markers were assayed with qPCR. Results showed comparable persistence of DNA between...
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Abstract: Environmental DNA (eDNA) is DNA that has been released by an organism into its environment, such that the DNA can be found in air, water, or soil. In aquatic systems, eDNA has been shown to provide a sampling approach that is more sensitive for detecting target organisms faster, and less expensively than previous approaches. However, eDNA needs to be sampled in a manner that has been tested and found effective and, because single copies of target DNA are detected reliably, rigorous procedures must be designed to avoid sample contamination. Here we provide the details of a sampling protocol designed for detecting fish. This protocol, or very similar prototypes, has been used to collect data reported in...
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Data describe designed environmental DNA (eDNA) experiments for the estimation of eDNA shedding rates for the spectaclecase (Cumberlandia monodonta), mucket (Actinonaias ligamentina), and fatmucket (Lampsilis siliquoidea) from water samples collected in the laboratory under differing laboratory conditions. Shedding rates were tested under variable mussel biomass, diet, and temperature. Parameters described include descriptions of laboratory collected water samples that were tested to estimate concentration of spectaclecase, mucket or fatmucket DNA qPCR results, and associated quality assurance measurements.
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The Great Plains Landscape Conservation Cooperative (GPLCC, https://www.fws.gov/science/catalog) is a partnership that provides applied science and decision support tools to assist natural resource managers conserve plants, fish and wildlife in the mid- and short-grass prairie of the southern Great Plains. It is part of a national network of public-private partnerships — known as Landscape Conservation Cooperatives (LCCs, http://www.fws.gov/science/shc/lcc.html) — that work collaboratively across jurisdictions and political boundaries to leverage resources and share science capacity. The Great Plains LCC identifies science priorities for the region and helps foster science that addresses these priorities to support...
The Great Plains Landscape Conservation Cooperative (GPLCC, https://www.fws.gov/science/catalog) is a partnership that provides applied science and decision support tools to assist natural resource managers conserve plants, fish and wildlife in the mid- and short-grass prairie of the southern Great Plains. It is part of a national network of public-private partnerships — known as Landscape Conservation Cooperatives (LCCs, http://www.fws.gov/science/shc/lcc.html) — that work collaboratively across jurisdictions and political boundaries to leverage resources and share science capacity. The Great Plains LCC identifies science priorities for the region and helps foster science that addresses these priorities to support...
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These data are amplicon sequence variant (ASV) counts of marine fish derived from environmental DNA (eDNA) sampling targeting the 12S rDNA, V5 region. DNA was extracted from filtered water samples taken off coastal New Jersey during the 2019 New Jersey Ocean Trawl Survey. Sampling corresponded to the New Jersey Ocean Trawl Survey efforts, but trawl data are not included here.
Categories: Data; Tags: Fish and Fisheries, Trawl survey, eDNA
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Data describe designed environmental DNA (eDNA) experiments for the estimation of eDNA shedding rates for the spectaclecase (Cumberlandia monodonta) and mucket (Actinonaias ligamentina) from water samples collected in the laboratory at different water temperatures. Parameters described include descriptions of laboratory collected water samples that were tested to estimate concentration of spectaclecase, mucket or fatmucket DNA qPCR results, and associated quality assurance measurements.
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This data archive includes R code and data for reproducing the analyses and figures in Lafferty, Metabarcoding is (usually) more cost effective than seining or qPCR for detecting tidewater gobies and other estuarine fishes. To view the supplementary tables, open the Fig&TableSuppl.docx file. This file also includes the manuscript figures and tables and some explanatory text about how to generate them. To reproduce the figures, open the Fig&TableCode.Rmd in R studio and be sure the needed csv files included in the Dryad repository are in the working directory. The data files include more information than used in the analyses and can be used for other purposes. The code is not software, nor is it intended as an...
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The Great Plains Landscape Conservation Cooperative (GPLCC, https://www.fws.gov/science/catalog) is a partnership that provides applied science and decision support tools to assist natural resource managers conserve plants, fish and wildlife in the mid- and short-grass prairie of the southern Great Plains. It is part of a national network of public-private partnerships — known as Landscape Conservation Cooperatives (LCCs, http://www.fws.gov/science/shc/lcc.html) — that work collaboratively across jurisdictions and political boundaries to leverage resources and share science capacity. The Great Plains LCC identifies science priorities for the region and helps foster science that addresses these priorities to support...
    map background search result map search result map Hillshade for the Great Plains Landscape Conservation Cooperative LANDFIRE Aspect for the Great Plains Landscape Conservation Cooperative Elevation Derivatives for National Applications (EDNA) Aspect for the Great Plains Landscape Conservation Cooperative Elevation Derivatives for National Applications (EDNA) Slope for the Great Plains Landscape Conservation Cooperative (GPLCC) A Protocol for Collecting Environmental DNA Samples From Streams Puuhonua o Honaunau and Kaloko-Honokohau National Historical Parks, carbon dioxide treatment and qPCR eDNA assays for eradicating and monitoring invasive fish in anchialine pools, 2019-2022 (ver. 2.0, July 2023) eDNA observations, concurrent with trawl survey, of marine fish in coastal New Jersey, USA 2019 Environmental DNA shedding rates in laboratory conditions for Cumberlandia monodonta, Actinonaias ligamentina, and Lampsilis siliquoidea. Biomass experiments with Cumberlandia monodonta, Actinonaias ligamentina, and Lampsilis siliquoidea Temperature experiments with Actinonaias ligamentina and Cumberlandia monodonta Food availability experiments with Actinonaias ligamentina and Lampsilis siliquoidea Tidewater goby and estuarine fish records from seining, qPCR and metabarcoding data for Southern California Estuaries in 2023 Environmental DNA shedding rates in laboratory conditions for Cumberlandia monodonta, Actinonaias ligamentina, and Lampsilis siliquoidea. Biomass experiments with Cumberlandia monodonta, Actinonaias ligamentina, and Lampsilis siliquoidea Temperature experiments with Actinonaias ligamentina and Cumberlandia monodonta Food availability experiments with Actinonaias ligamentina and Lampsilis siliquoidea A Protocol for Collecting Environmental DNA Samples From Streams Tidewater goby and estuarine fish records from seining, qPCR and metabarcoding data for Southern California Estuaries in 2023 Puuhonua o Honaunau and Kaloko-Honokohau National Historical Parks, carbon dioxide treatment and qPCR eDNA assays for eradicating and monitoring invasive fish in anchialine pools, 2019-2022 (ver. 2.0, July 2023) eDNA observations, concurrent with trawl survey, of marine fish in coastal New Jersey, USA 2019 Elevation Derivatives for National Applications (EDNA) Aspect for the Great Plains Landscape Conservation Cooperative LANDFIRE Aspect for the Great Plains Landscape Conservation Cooperative Hillshade for the Great Plains Landscape Conservation Cooperative Elevation Derivatives for National Applications (EDNA) Slope for the Great Plains Landscape Conservation Cooperative (GPLCC)