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This dataset includes quantifications of bigheaded carp DNA found in water samples collected from the Wabash River along transects at 3 sites over time. The samples were collected at 18 equidistant points in a transect across the river at each site. Samples were collected in 2013 on May 29, 30, 31, June 5, 6, 7, 8, 9, 10, 11, 16, 17, 19, 20, 21, and 22. Quantitative polymerase chain reaction (qPCR) was performed to determine the DNA concentrations in two replicates using the bigheaded carp assay defined in Merkes and others 2014.
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Eggs were collected in the Upper Mississippi main stem (Pool 9 and Pool 11) during the summer of 2013. Using previously published morphological characteristics, eggs were positively identified as belonging to an invasive Asian carp genus. A subsample of these eggs was subsequently analyzed using molecular methods to determine species identity. Genetic identification of a total of 41 eggs was attempted using the cytochrome c oxidase 1 (COI) gene. Due to the preservation technique used (formalin) and resulting DNA degradation, sequences from only 17 individuals could be recovered. In all cases, non-carp cyprinids were identified as the most likely species identity (usually a Notropis spp.). In previously published...
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Abstract: Environmental DNA (eDNA) is DNA that has been released by an organism into its environment, such that the DNA can be found in air, water, or soil. In aquatic systems, eDNA has been shown to provide a sampling approach that is more sensitive for detecting target organisms faster, and less expensively than previous approaches. However, eDNA needs to be sampled in a manner that has been tested and found effective and, because single copies of target DNA are detected reliably, rigorous procedures must be designed to avoid sample contamination. Here we provide the details of a sampling protocol designed for detecting fish. This protocol, or very similar prototypes, has been used to collect data reported in...
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Data is a spreadsheet of the number of copies of Zebra Mussel DNA detected and the number of positive detections for Zebra Mussel DNA from water samples collected over 6 different substrates, at 4 depths in 2 lakes. The ash-free dry weight of the mussels at each of the sites is also included for each sampling location.
This dataset includes quantifications of bigheaded carp DNA found in water samples collected from the Wabash River along transects at 3 sites over time. The samples were collected at 18 equidistant points in a transect across the river at each site. Samples were collected in 2013 on May 29, 30, 31, June 5, 6, 7, 8, 9, 10, 11, 16, 17, 19, 20, 21, and 22. Quantitative polymerase chain reaction (qPCR) was performed to determine the DNA concentrations in two replicates using the bigheaded carp assay defined in Merkes and others 2014.
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The Great Plains Landscape Conservation Cooperative (GPLCC, https://www.fws.gov/science/catalog) is a partnership that provides applied science and decision support tools to assist natural resource managers conserve plants, fish and wildlife in the mid- and short-grass prairie of the southern Great Plains. It is part of a national network of public-private partnerships — known as Landscape Conservation Cooperatives (LCCs, http://www.fws.gov/science/shc/lcc.html) — that work collaboratively across jurisdictions and political boundaries to leverage resources and share science capacity. The Great Plains LCC identifies science priorities for the region and helps foster science that addresses these priorities to support...
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The Great Plains Landscape Conservation Cooperative (GPLCC, https://www.fws.gov/science/catalog) is a partnership that provides applied science and decision support tools to assist natural resource managers conserve plants, fish and wildlife in the mid- and short-grass prairie of the southern Great Plains. It is part of a national network of public-private partnerships — known as Landscape Conservation Cooperatives (LCCs, http://www.fws.gov/science/shc/lcc.html) — that work collaboratively across jurisdictions and political boundaries to leverage resources and share science capacity. The Great Plains LCC identifies science priorities for the region and helps foster science that addresses these priorities to support...
This dataset includes quantifications of bigheaded carp DNA found in water samples collected from the Wabash River along transects at 3 sites over time. The samples were collected at 18 equidistant points in a transect across the river at each site. Samples were collected in 2013 on May 29, 30, 31, June 5, 6, 7, 8, 9, 10, 11, 16, 17, 19, 20, 21, and 22. Quantitative polymerase chain reaction (qPCR) was performed to determine the DNA concentrations in two replicates using the bigheaded carp assay defined in Merkes and others 2014.
This dataset includes quantifications of bigheaded carp DNA found in water samples collected from the Wabash River along transects at 3 sites over time. The samples were collected at 18 equidistant points in a transect across the river at each site. Samples were collected in 2013 on May 29, 30, 31, June 5, 6, 7, 8, 9, 10, 11, 16, 17, 19, 20, 21, and 22. Quantitative polymerase chain reaction (qPCR) was performed to determine the DNA concentrations in two replicates using the bigheaded carp assay defined in Merkes and others 2014.
    map background search result map search result map Elevation Derivatives for National Applications (EDNA) Aspect for the Great Plains Landscape Conservation Cooperative Elevation Derivatives for National Applications (EDNA) Slope for the Great Plains Landscape Conservation Cooperative (GPLCC) Asian carp eggs cannot be distinguished from other cyprinid species on the basis of morphology alone: Supporting Data Wabash River, Indiana bigheaded carps environmental DNA: Data A Protocol for Collecting Environmental DNA Samples From Streams Environmental DNA mapping of Zebra Mussel populations: Data Wabash River, Indiana bigheaded carps environmental DNA: Data Wabash River, Indiana bigheaded carps environmental DNA: Data Wabash River, Indiana bigheaded carps environmental DNA: Data A Protocol for Collecting Environmental DNA Samples From Streams Asian carp eggs cannot be distinguished from other cyprinid species on the basis of morphology alone: Supporting Data Wabash River, Indiana bigheaded carps environmental DNA: Data Wabash River, Indiana bigheaded carps environmental DNA: Data Wabash River, Indiana bigheaded carps environmental DNA: Data Wabash River, Indiana bigheaded carps environmental DNA: Data Environmental DNA mapping of Zebra Mussel populations: Data Elevation Derivatives for National Applications (EDNA) Aspect for the Great Plains Landscape Conservation Cooperative Elevation Derivatives for National Applications (EDNA) Slope for the Great Plains Landscape Conservation Cooperative (GPLCC)