Filters: Tags: eDNA (X)
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This data set contains imagery from the National Agriculture Imagery Program (NAIP). The NAIP program is administered by USDA FSA and has been established to support two main FSA strategic goals centered on agricultural production. These are, increase stewardship of America's natural resources while enhancing the environment, and to ensure commodities are procured and distributed effectively and efficiently to increase food security. The NAIP program supports these goals by acquiring and providing ortho imagery that has been collected during the agricultural growing season in the U.S. The NAIP ortho imagery is tailored to meet FSA requirements and is a fundamental tool used to support FSA farm and conservation programs....
This dataset includes quantifications of bigheaded carp DNA found in water samples collected from the Wabash River along transects at 3 sites over time. The samples were collected at 18 equidistant points in a transect across the river at each site. Samples were collected in 2013 on May 29, 30, 31, June 5, 6, 7, 8, 9, 10, 11, 16, 17, 19, 20, 21, and 22. Quantitative polymerase chain reaction (qPCR) was performed to determine the DNA concentrations in two replicates using the bigheaded carp assay defined in Merkes and others 2014.
Categories: Data;
Types: Citation,
Map Service,
OGC WFS Layer,
OGC WMS Layer,
OGC WMS Service;
Tags: Americus,
Asian carp,
Bigheaded carps,
Environmental DNA,
French Post,
Interest in the field of environmental DNA (eDNA) is growing rapidly and eDNA surveys are becoming an important consideration for aquatic resource managers dealing with invasive species. However, in order for eDNA monitoring to mature as a research and management tool, there are several critical knowledge gaps that must be filled. One such gap is the fate of eDNA materials in the aquatic environment. Understanding the environmental factors that influence the decay of eDNA and how these factors impact detection probabilities over time and space could have significant implications for eDNA survey design and data interpretation. Here we experimentally explore eDNA decay in waste materials and reproductive cells obtained...
Categories: Data;
Types: Citation;
Tags: Aquatic Biology,
Ecology,
Environmental Health,
Genetics,
Missouri,
Data describe a designed environmental DNA (eDNA) survey for the detection of longnose darter (Percina nasuta) in field collected water samples. Parameters described include the limit of detection and limit of quantification of the assay; a list of freshwater fish species tissue samples that were used to test specificity of the assay; and field collected water samples that were tested to detect the presence of longnose darter DNA. Samples were collected from sites on the St. Francis river, Missouri in 2018.
USGS Historical Quadrangle in GeoPDF.
Data describe designed environmental DNA (eDNA) experiments for the estimation of eDNA shedding rates for the spectaclecase (Cumberlandia monodonta), mucket (Actinonaias ligamentina), and fatmucket (Lampsilis siliquoidea) from water samples collected in the laboratory under differing laboratory conditions. Shedding rates were tested under variable mussel biomass, diet, and temperature. Parameters described include descriptions of laboratory collected water samples that were tested to estimate concentration of spectaclecase, mucket or fatmucket DNA qPCR results, and associated quality assurance measurements.
Categories: Data;
Tags: Columbia Environmental Research Center,
climatologyMeteorologyAtmosphere,
eDNA,
eDNA Shedding rates,
environmental DNA,
This data set contains imagery from the National Agriculture Imagery Program (NAIP). The NAIP program is administered by USDA FSA and has been established to support two main FSA strategic goals centered on agricultural production. These are, increase stewardship of America's natural resources while enhancing the environment, and to ensure commodities are procured and distributed effectively and efficiently to increase food security. The NAIP program supports these goals by acquiring and providing ortho imagery that has been collected during the agricultural growing season in the U.S. The NAIP ortho imagery is tailored to meet FSA requirements and is a fundamental tool used to support FSA farm and conservation programs....
This data set contains imagery from the National Agriculture Imagery Program (NAIP). The NAIP program is administered by USDA FSA and has been established to support two main FSA strategic goals centered on agricultural production. These are increase stewardship of America's natural resources while enhancing the environment, and to ensure commodities are procured and distributed effectively and efficiently to increase food security. The NAIP program supports these goals by acquiring and providing ortho imagery that has been collected during the agricultural growing season in the U.S. The NAIP ortho imagery is tailored to meet FSA requirements and is a fundamental tool used to support FSA farm and conservation programs....
USGS Historical Quadrangle in GeoPDF.
We conducted a study to test the factors related to detectability of two invasive aquatic plants (Egeria densa and Myriophyllym spicatum) using environmental DNA (eDNA), over extended periods of time, and specifically examined how plant growth stage and abundance relates to eDNA detection in semi-natural and natural conditions. This dataset is from sampling performed in summer of 2018 in lakes with varying species abundances, and a subset of lakes were re-sampled to test temporal variability in detection.
Categories: Data;
Tags: Aquatic Biology,
Downloadable Data,
Ecology,
Egeria densa,
Myriophyllym spicatum,
USGS Historical Quadrangle in GeoPDF.
USGS Historical Quadrangle in GeoPDF.
This data set was collected to provide examples and aid in developing a standardized way of determining LOD and LOQ for eDNA assays and has 3 data files. GEDWG_LOD_DATA3.csv is raw qPCR data from multiple labs running multiple standards of known concentration for eDNA assays they regularly use. Comparison-Data.csv is the merged data output from running a generic LOD/LOQ calculator script multiple times with different LOD model settings. The generic LOD/LOQ calculator script is available at: https://github.com/cmerkes/qPCR_LOD_Calc, and details about the multiple settings used are commented in the analysis script available at: https://github.com/cmerkes/LOD_Analysis
The Great Plains Landscape Conservation Cooperative (GPLCC, https://www.fws.gov/science/catalog) is a partnership that provides applied science and decision support tools to assist natural resource managers conserve plants, fish and wildlife in the mid- and short-grass prairie of the southern Great Plains. It is part of a national network of public-private partnerships — known as Landscape Conservation Cooperatives (LCCs, http://www.fws.gov/science/shc/lcc.html) — that work collaboratively across jurisdictions and political boundaries to leverage resources and share science capacity. The Great Plains LCC identifies science priorities for the region and helps foster science that addresses these priorities to support...
Categories: Data;
Types: Downloadable,
GeoTIFF,
Map Service,
Raster;
Tags: Digital Elevation Model,
EDNA,
GPLCC,
Great Plains Landscape Conservation Cooperative,
Hydrologic Derivative,
The Great Plains Landscape Conservation Cooperative (GPLCC, https://www.fws.gov/science/catalog) is a partnership that provides applied science and decision support tools to assist natural resource managers conserve plants, fish and wildlife in the mid- and short-grass prairie of the southern Great Plains. It is part of a national network of public-private partnerships — known as Landscape Conservation Cooperatives (LCCs, http://www.fws.gov/science/shc/lcc.html) — that work collaboratively across jurisdictions and political boundaries to leverage resources and share science capacity. The Great Plains LCC identifies science priorities for the region and helps foster science that addresses these priorities to support...
Categories: Data;
Types: Downloadable,
GeoTIFF,
Map Service,
Raster;
Tags: Aspect,
Colorado,
Downslope Direction,
EDNA,
GPLCC,
Data describe designed environmental DNA (eDNA) experiments for the estimation of eDNA shedding rates for mucket (Actinonaias ligamentina) and fatmucket (Lampsilis siliquoidea) from water samples collected in the laboratory under differing food avilability scenarios. Parameters described include descriptions of laboratory collected water samples that were tested to estimate concentration of spectaclecase, mucket or fatmucket DNA qPCR results, and associated quality assurance measurements.
Categories: Data;
Tags: Columbia Environmental Research Center,
climatologyMeteorologyAtmosphere,
eDNA,
eDNA Shedding rates,
environmental DNA,
This file contains one of many raster grids of the Elevation Derivatives for National Applications (EDNA), a multi-layered database that provides systematic and consistent topographically-derived hydrologic derivatives. The slope grid was generated from the filled DEM using the "slope" function. The grid was created with the degree, not percent, slope parameter.
Categories: Data;
Types: Downloadable;
Tags: EDNA,
Federal Government,
Hydrologic Derivative,
LANDFIRE,
Raster digital data,
The data sets contains hierarchical taxonomic identification information for DNA sequences detected in water samples from 5 locations.
Categories: Data;
Tags: Aquatic Biology,
Decision-Making Support and Tools,
Ecology,
Mammals,
Nevada,
This data set contains imagery from the National Agriculture Imagery Program (NAIP). The NAIP program is administered by USDA FSA and has been established to support two main FSA strategic goals centered on agricultural production. These are increase stewardship of America's natural resources while enhancing the environment, and to ensure commodities are procured and distributed effectively and efficiently to increase food security. The NAIP program supports these goals by acquiring and providing ortho imagery that has been collected during the agricultural growing season in the U.S. The NAIP ortho imagery is tailored to meet FSA requirements and is a fundamental tool used to support FSA farm and conservation programs....
Eggs were collected in the Upper Mississippi main stem (Pool 9 and Pool 11) during the summer of 2013. Using previously published morphological characteristics, eggs were positively identified as belonging to an invasive Asian carp genus. A subsample of these eggs was subsequently analyzed using molecular methods to determine species identity. Genetic identification of a total of 41 eggs was attempted using the cytochrome c oxidase 1 (COI) gene. Due to the preservation technique used (formalin) and resulting DNA degradation, sequences from only 17 individuals could be recovered. In all cases, non-carp cyprinids were identified as the most likely species identity (usually a Notropis spp.). In previously published...
Categories: Data;
Types: Citation,
Map Service,
OGC WFS Layer,
OGC WMS Layer,
OGC WMS Service;
Tags: Mississippi River,
USGS Science Data Catalog (SDC),
eDNA,
environmental DNA
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