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This study addressed the potential ability to link landscape indices to stream water quality in a predominately agricultural landscape located in the Mississinewa River watershed, East-Central Indiana. A methodology for developing and analyzing landscape indices using a GIS and remotely sensed and geospatial data was applied to 30 Hydrologic Unit Code (HUC) 14-digit subwatersheds. Six indices, three representing natural area extent characteristics and three representing natural area disturbance characteristics were developed. The resulting indices were then tested to determine if they could be linked to water quality variables (Total Phosphorus, Nitrate, E.COLI, and macroinvertebrate [EPT/C] Ephemeroptera, Plecoptera,...