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This study examined trace elements concentrations and macroinvertebrate community structure at 32 sites in 22 streams in Colorado. Sites affected by mining activities (mining sites) and sites that were minimally disturbed (nonmining sites) were selected for the assessment. Water and transplanted aquatic bryophyte samples were analyzed for trace elements. Macroinvertebrate samples were collected to assess the effects of trace elements on the aquatic community of the stream. All samples of aquatic bryophytes had detectable concentrations of Cd, Cu, Pb and Zn. Principal components analysis of chemical and physical properties classified sites into three groups. The first group represented sites that were unaffected...