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Data are included for field surveys conducted for freshwater mussels within the Pine Creek watershed in the Mid-Atlantic region of the United States. Timed search (qualitative) surveys were conducted in ~200 meter sections of river between 1995 and 2009; The number of mussels of each species and associated search time were recorded for each survey section to determine a catch per unit effort (CPUE; number of individuals found per person hour).
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This database contains aquatic benthic macroinvertebrate abundance data derived from samples collected from 33 75-meter stream site locations within the Delaware Water Gap National Recreation area in September of 2006. The database contains two separate table files (i.e., entities) in csv format: 1) Macroinvertebrate site abundance data and 2) site location data. For macroinvertebrate data, six samples were collected within 2-3 riffle areas within each site location with Portable Invertebrate Box Samplers (0.1-m2 sample area, 350 micron mesh collecting nets). Where possible, organisms were identified to the genus taxonomic level using a stereoscope except for organisms of the family Chironomidae and class Oliogochaeta,...
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Rapid advances in video technology are enabling new strategies for species abundance estimation. Here we provide estimates of fish abundance derived from video data collected in a series of stream pools in Shenandoah National Park, Virginia (n=41). Two 360-degree cameras were simultaneously used at each pool site where 15-minutes of underwater footage was collected. Environmental data are provided describing pool morphology, canopy cover, and fish cover (boulders, large woody debris, and undercut banks).
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Brook trout is a species of conservation concern in the eastern US. In 2016, we evaluated brook trout movement patterns in response to food and cover treatments in an experimental stream system using RFID monitoring techniques at the USGS Leetown Science Center in Kearneysville, WV. Brook trout were tagged with 12mm HDX Passive Integrated Transponder (PIT) tags and monitored with Multi-Antenna HDX Readers within each experimental stream riffle. We collected associated data on stream temperature using HOBO temperature loggers (Onset Pro V2) and water quality parameters such as dissolved oxygen, conductivity and pH (YSI Professional Plus multiparameter instrument).
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This dataset consists of historical estimates and future projections of land use and climate data summarized within the 1:100,000 National Hydrography Dataset Version 2 (NHDPlusV2) framework for catchments and upstream accumulated watersheds. Historical land use data are for the year 2005 and future land use projections are for the years 2030, 2060, and 2090. The projections offer a unique combination of thematic detail (17 land-use and land-cover classes). Historical climate estimates are averaged over the time period 1980-1999 and future climate projections are averaged over 20-year periods centered around the years 2030, 2060, and 2090. Climate data include seasonal measures of average air temperature (℃) and...
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Data are included for laboratory studies evaluating the thermal biology of several freshwater mussel species and their host fish including dwarf wedgemussel (Alasmidonta heterodon), brook floater (Alasmidonta varicosa), creeper (Strophitus undulatus), eastern elliptio mussel (Elliptio complanata), tesselated darter (Etheostoma olmstedi), and slimy sculpin (Cottus cognatus). Thermal endpoints include critical thermal maximum, temperature preference, oxygen consumption rates, and clearance rates of organisms acclimated to a range of temperature treatments.
The Fish Health Branch investigates persistent and emergent health issues associated with genetics, pathogens and environmental stressors that undermine conservation and restoration of interjurisdictional populations including depleted, threatened, or endangered fishes and aquatic organisms. VISION STATEMENT The Fish Health Branch conducts integrated research to understand and solve emerging and known disease problems, both infectious and non-infectious, affecting fish and other aquatic resources. Resolution of these health problems is accomplished through research utilizing a variety of disciplines including microbiology, parasitology, virology, immunology, histopathology, molecular biology, and aquatic toxicology...
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A laboratory study was conducted at the Northern Appalachian Research Laboratory (Wellsboro, PA) on the ability to manipulate photoperiod and water temperatures to determine its effects on Eastern elliptio freshwater mussel glochidia production. Brooding mussels were collected from Pine Creek, Tioga County, PA in late December and were housed in the laboratory in groups subjected to one of four environmental treatments: natural temperature and photoperiod, 6 week delay in natural conditions, 12 week delay in natural conditions, and natural temperature and photoperiod with a winter low of 10°C. In this study, "natural" conditions refer to light and temperature conditions that mimic what mussels would have been exposed...
The Southern Appalachian Research Branch (SARB) provides research support for management of wildlife for federal lands within the Department of the Interior, including migratory species, inter-jurisdictional species, endangered species, invasive species, and species of cultural importance to Native Americans; maintaining an emphasis on upland ecosystems in the southern Appalachian Highlands. The SARB conducts research in landscape modeling to predict the ecological consequences of anthropogenic impacts to the southern Appalachians and provides expertise in wildlife habitat and population analysis with an emphasis on the management of large mammals with the goal of demonstrating concepts of sustainable wildlife population...
The Northern Appalachian Research Branch (NARB) provides research support for management of fisheries and aquatic resources for federal lands within the Department of the Interior (National Park Service, Fish & Wildlife Service, Bureau of Land Management), migratory species, inter-jurisdictional species, endangered species, invasive species, and species of cultural importance to Native Americans. The NARB boasts an outdoor raceway system, wet and dry labs, library, and conference facilities. With an ample supply of water, the facility is able to perform large-scale laboratory and field research. The NARB maintains a diversified research program in aquatic ecology, fisheries biology, landscape ecology, genetics,...
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Data are included for two types of field surveys conducted for freshwater mussels within the Neversink River in the Mid-Atlantic region of the United States. Timed search (qualitative) surveys were conducted in consecutive ~200 meter sections of river in 2006 and 2007; a subset of these sections were re-surveyed qualitatively in 2009. The number of mussels of each species and associated search time were recorded for each survey section to determine a catch per unit effort (CPUE; number of individuals found per person hour). Within the 2009 qualitatively surveyed areas, ten ~200 meter sections of stream were selected in which to conduct quantitative surveys to estimate mussel abundance. Quantitative survey methods...
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The files in this data release are the processed DNA consensus sequence alignment files for the chelonid herpesvirus 5 (ChHV-5) genome plus 66 open reading frames representing protien-coding genes. DNA was extracted from tumor biopsies from green sea turtles (Chelonias mydas) with fibropapillomatosis sampled from Florida and Hawaii coastal waters. We used high-throughput short-read sequencing (Illumina MiSeq and Ion Torrent Personal Genome Machine) of long-range PCR products amplified from tumor tissue using primers designed from the single available ChHV5 reference genome from a Hawaii green sea turtle. The raw sequence data is available at the NCBI Sequence Read Archive under bioproject number PRJNA360405, including...
Tags: Class Reptilia, DNA sequencing, Earth, Oceans, Atlantic, North Atlantic, Earth, Oceans, Pacific, Family Cheloniidae, All tags...
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This data represents environmental attributes collected at or around field sample locations (groundwater piezometers or water wells) that were used in a study of impacts from a historic railroad embankment on wetland vegetation. GPS surveys were used to map piezometer locations, elevations, and the elevation of the ground surface in proximity to the piezometer. Water surface elevations and peat depths were measured in the field at the piezometers. Piezometer locations were also used to center a 20m x 20m vegetation survey field plot from which attributes on mean surface elevations and vegetation heights were summarized using lidar data.
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This data was collected over the course of two years, from 2016 to 2017, within the flume facility laboratory. The research project that was conducted evaluated different fishway entrance designs and their effect on the passage of adult American shad. The data consists of 15 columns and 1925 rows. Each row entry pertains to the performance of a single fish during one of 64 trials over the two year period. Columns give information on the treatment condition (e.g. entrance gate designs, headwater/tailwater elevations), water quality (e.g., water temperature, turbidity), fish specific information (e.g. sex, fork length, handling), passage time, and the censor value used in the statistical analysis.
The Aquatic Ecology Branch conducts science for ecological conservation at a landscape scale. Our work emphasizes population, community, and ecosystem ecology, addressing research questions of specific importance for the eastern United States and Appalachian region. Our capabilities include genetics and genomics, landscape ecology, stream fish ecology, GIS and remote sensing, and statistical modeling. We apply a variety of experimental and statistical approaches to assess ecological status and trends, and to understand the underlying causes of ecological resilience and change.
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Data are included from three parts of the research in the Delaware River focusing on three sites known to support populations of Alasmidonta heterodon (dwarf wedgemussel). Site Rating Curve Data was data used to create unique river stage to river discharge models for each of three sites used to model how site conditions varied compared to USGS River Gage conditions. Site Bathymetry and Water Depth data were used to create river bottom topography and water depth maps for the three sites. A compilation of 6pm river temperature data were used to create temperature prediction models for each of the three sites.
With their unique life histories, anadromous fishes require special consideration in understanding their biology and developing management strategies. During the Industrial Revolution, many populations of anadromous fishes were severely depleted or extirpated by damming, pollution or alteration of their migratory or spawning habitats. Some measures were taken in the late 1880s to provide access for anadromous fishes around dams by construction of rudimentary fishways, or by stocking fish into habitats that historically supported large runs. However, without a complete understanding of the ecology, life history and environmental needs of these species, many of these efforts failed to restore naturally reproducing...
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Intersex in small- and largemouth bass has been observed in the upper Chesapeake Bay Watershed since 2003. This observation is indicative of exposure to estrogenic endocrine disrupting chemicals. In order to complement biological data sets and better identify the spatial and temporal nature of this end-point, discrete grab water samples were collected throughout the upper Chesapeake Bay Watershed for the evaluation of estrogenic activity. Data reported here are estrogenicity values derived from bioassay screening of OASIS-HLB extracts discrete grab water samples. Estrogenicity was determined using a bioluminescent yeast estrogen screen. Here we report estrogenicity data for selected sites in the Chesapeake Bay water...
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Data were collected on American glass eels (Anguilla rostrata) to determine optimal thermal conditions for laboratory rearing and aquaculture. American glass eels (Anguilla rostrata) were housed in buckets for 3 weeks at 14, 18, 22, or 26°C (n=5 buckets/treatment) to determine optimal juvenile rearing temperature in the laboratory. Initial weight (g) was recorded for each bucket. During the 3 week experimental period, data was collected on water temperature (°C), mortality, and the presence of gas bubbles. Water samples were taken to measure ammonia (mg/L) following the completion of the experiment. Final eel length (mm) and wet weight (g) were recorded for all eels in the study.


    map background search result map search result map Conte Anadromous Fish Branch Site bathymetry, water temperature, and site rating curves data for three sites in the Delaware River (2005) Estrogen Equivalents of Surface Water in the Upper Chesapeake Bay Watershed (2008 - 2011) Targeted amplicon resequencing of chelonid alphaherpesvirus 5 from tumor samples collected from Chelonia mydas (green turtle) inhabiting Hawaiian and Florida coastal waters Laboratory study on the effects of rearing temperature on American glass eels, Anguilla rostrata Brook trout movement data related to cover and forage in an experimental stream system with associated water quality parameters Flume facility data collection at the S.O. Conte Anadromous Fish Research Laboratory in Turners Falls, MA for the 2016-2017 Fishway Entrance Gate Project Laboratory studies on the thermal biology of freshwater mussels and their host fish species Chesapeake Bay Watershed historical and future projected land use and climate data summarized for NHDPlusV2 catchments Laboratory study investigating the effects of seasonal temperature and photoperiod manipulation on Eastern elliptio reproduction Benthic macroinvertebrates abundance data for the study of urbanization effects in the Delaware Water Gap National Recreation Area, (2006) Environmental data collected at piezometer field plot locations used to study hydrologic impacts on vegetation due to historic rail road embankment at Canaan Valley NWR Video data for trout abundance estimation Pine Creek freshwater mussel qualitative survey data 1995 through 2009 Neversink River freshwater mussel surveys: 2006-2009 Brook trout movement data related to cover and forage in an experimental stream system with associated water quality parameters Laboratory study on the effects of rearing temperature on American glass eels, Anguilla rostrata Laboratory study investigating the effects of seasonal temperature and photoperiod manipulation on Eastern elliptio reproduction Flume facility data collection at the S.O. Conte Anadromous Fish Research Laboratory in Turners Falls, MA for the 2016-2017 Fishway Entrance Gate Project Laboratory studies on the thermal biology of freshwater mussels and their host fish species Environmental data collected at piezometer field plot locations used to study hydrologic impacts on vegetation due to historic rail road embankment at Canaan Valley NWR Site bathymetry, water temperature, and site rating curves data for three sites in the Delaware River (2005) Video data for trout abundance estimation Benthic macroinvertebrates abundance data for the study of urbanization effects in the Delaware Water Gap National Recreation Area, (2006) Neversink River freshwater mussel surveys: 2006-2009 Pine Creek freshwater mussel qualitative survey data 1995 through 2009 Chesapeake Bay Watershed historical and future projected land use and climate data summarized for NHDPlusV2 catchments Estrogen Equivalents of Surface Water in the Upper Chesapeake Bay Watershed (2008 - 2011) Targeted amplicon resequencing of chelonid alphaherpesvirus 5 from tumor samples collected from Chelonia mydas (green turtle) inhabiting Hawaiian and Florida coastal waters