Folders: ROOT > ScienceBase Catalog > Chesapeake Bay Endocrine Disrupting Chemicals ( Show all descendants )
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This dataset consists of 262 variables which describe various known and suspected point and non-point sources of contaminants and endocrine disrupting compounds (EDCs) throughout the Chesapeake Bay Watershed. Contaminant data was summarized to the NHDPlus Version 2.1 catchment level (1:100K). Contaminant data summarized span a time range of 2001 to 2016 and include regulated facilities, pesticides, manure and biosolids application data, mercury deposition, animal feeding applications, septic systems, landfills, and land use and land cover. These data are presented in a comma separated file, which includes all variables summarized and the NHDPlus Version 2.1 FEATUREID field (also known as COMID). The FEATUREID field...
Categories: Data,
Data Release - Revised;
Types: Citation;
Tags: Aquatic Biology,
Chesapeake Bay,
Chesapeake Bay Basin,
Delaware,
Discharge Monitoring Report (DMR),
We have adapted two commonly used human breast luciferase transactivation cell bioassays, the recently re-named estrogen agonist/antagonist screening VM7Luc4E2 cell bioassay (previously designated BG1Luc4E2) and the androgen/glucocorticoid screening MDA-kb2 cell bioassay, to 384-well formats for HTS of endocrine-active substances. This cost-saving adaptation includes a fast, accurate, and easy measurement of protein amount in each well via the fluorescamine assay (in the form of relative fluorescence units, RFU which are then converted to microgram protein using bovine serum albumin standards) with which to normalize luciferase activity (in the form of relative light units, RLU) of cell lysates without requiring...
Categories: Data;
Types: Map Service,
OGC WFS Layer,
OGC WMS Layer,
OGC WMS Service;
Tags: Columbia Environmental Research Center,
Missouri,
USGS Science Data Catalog (SDC),
bioassay,
cells,
These data represent water and bed sediment samples analyzed for a variety of organic compounds. The samples were collected in streams and rivers in the Chesapeake Bay watershed from 2006-2014. Water samples were collected from 61 sites and analyzed for hormones (SH2434 method; Tables 1A and 1B), pharmaceuticals (SH2080 method; Tables 2A and 2B), wastewater indicators (SH1433 method; Tables 3A and 3B), and antibiotics (LCAB method; Tables 4A and 4B). Select water samples were analyzed at the U.S. Geological Survey National Water Quality Laboratory for pesticides (SH2001 method and SH2003 method; Tables 5A/5B and 6A/6B, respectively), wastewater indicators (SH4433 method; Tables 7A and 7B), pharmaceuticals (SH8244...
The data was gathered to document the extent and severity of biomarkers of exposure to estrogenic chemicals in smallmouth and largemouth bass. Samples were collected from 2004 through 2010 at sites in five watersheds within the Chesapeake Bay watershed. A total of 281 largemouth bass were collected from the Potomac, Susquehanna, Patuxent and Rappahannock rivers and 1,384 smallmouth bass from the Potomac, Susquehanna and James. Fish were collected by boat electroshocking, euthanized in the field, weighed, measured, a blood samples collected and a necropsy completed that included documenting any visible abnormalities and collecting tissues for histopathology. Plasma obtained from blood samples was analyzed for vitellogenin,...
Categories: Data;
Tags: Maryland,
Pennsylvania,
USGS Science Data Catalog (SDC),
Virginia,
West Virginia,
This data release presents chemical and biological results from investigations of water quality, fish endocrine disruption, and emergent insects in the Shenandoah River Watershed (Virginia and West Virginia, USA) conducted during 2014, 2015, and 2016. Multiple sampling campaigns were conducted at sites located throughout the Shenandoah River Watershed (Table 1). The complex inorganic and organic chemical characteristics of river waters and wastewater treatment plant effluents were characterized using 21 separate analytical methods at 7 laboratories (Tables 2, 3, and 4). To assess the relations between water composition and fish endocrine disruption, 21-day mobile laboratory adult fathead minnow exposure experiments...
Incedental wastewater reuse from streams that receive discharges from Wastewater Treatment Facilities (WWTF) has the potential to be a significant contributor of Endocrine Disrupting Chemicals. An ArcGIS model of WWTFs, NHDPlus Version 2 stream networks (USGS and EPA 2012), and gage stations across the Shenandoah River watershed was created to calculate accumulated wastewater in percent of streamflow (ACCWW%) and Predicted Environmental Concentrations (PECs) of select constituents. Virginia and West Virginia Pollutant Discharge Elimination System (VPDES, WVPDES) discharge facilities, outfall locations, and stream gages were spatially joined to the nearest river segment. Wastewater inputs from outfall locations were...
Categories: Data Release - Revised;
Types: Map Service,
OGC WFS Layer,
OGC WMS Layer,
OGC WMS Service;
Tags: Aquatic Biology,
Ecology,
Environmental Health,
Shenandoah River,
USGS Science Data Catalog (SDC),
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