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Filters: Tags: {"scheme":"USGS Biocomplexity Thesaurus"} (X) > Date Range: {"choice":"year"} (X) > partyWithName: Fort Collins Science Center (X)

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The data release details the samples, methods, and raw data used to generate high-quality genome assemblies for greater sage-grouse (Centrocercus urophasianus), white-tailed ptarmigan (Lagopus leucura), and trumpeter swan (Cygnus buccinator). The raw data have been deposited in the Sequence Read Archive (SRA) of the National Center for Biotechnology Information (NCBI), the authoritative repository for public biological sequence data, and are not included in this data release. Instead, the accessions that link to those data via the NCBI portal (www.ncbi.nlm.nih.gov) are provided herein. The release consists of a single file, sample.metadata.txt, which maps NCBI accessions to the samples sequenced and the different...
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High-throughput methods for identification of pollinator taxa are desirable to improve our understanding of pollinator distributions, population trends, and ecology. Genetic sequencing of taxonomically informative 'barcode' loci is one high-throughput strategy, which can be applied to individual specimens using Sanger technology and to complex mixtures using metabarcoding technology. This study generated Sanger sequencing data from morphologically identified specimens and metabarcoding data derived from pooled tissues. This data release consists of several files: 1. sample.metadata.txt, which contains sampling metadata and identifiers linking to sequence data that has been deposited in the Sequence Read Archive...
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Lake Sinai Viruses (LSV) are common ribonucleic acid (RNA) viruses of honey bees (Apis mellifera) that frequently reach high abundance but are not linked to overt disease. LSVs are genetically heterogeneous and collectively widespread, but despite frequent detection in surveys, the ecological and geographic factors structuring their distribution in A. mellifera are not understood. Even less is known about their distribution in other species. Better understanding of LSV prevalence and ecology have been hampered by high sequence diversity within the LSV clade. We developed a new genetic assay that detects all currently known lineages. We also performed pilot metagenetic sequencing to quantify the diversity of LSV...
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The dataset consists of genotypes (diploid base calls at variant sites) at 3,601 anonymous sites of the Arizona Toad (Anaxyrus microscaphus) nuclear genome. The genotyped samples are representative of the range of the species and its major population units, and the genotyped loci have a high degree of completeness. This data release consists of several files: 1. sample.metadata.txt, which contains sampling metadata and identifiers linking to sequence data that has been deposited in the Sequence Read Archive of the National Center for Biotechnology Information (NCBI). This database is authoritative and comprehensive for sharing high-throughput sequence data produced with public funds. All NCBI-derived accessions...
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Honey bees (Apis mellifera), a critical agricultural pollinator in many areas, have a high rate of infection with a large DNA virus, Apis mellifera filamentous virus (AmFV), yet little is known about its ecology or impact on honey bee colonies, other than its ubiquity and apparent low virulence. This study scanned over 5,000 public data sets to detect AmFV sequences in honey bees as well as a parasitic mite of honey bees, Varroa destructor, that is a potential vector of AmFV. The data release consists of these files: 1. AmFV.genome.assemblies.aligned.fas, which contains new AmFV draft genome sequences generated by this study aligned with existing reference genome accessions downloaded from the National Center for...
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This study generated genetic 'barcode' data using high-throughput sequencing to characterize pollen collected by the endangered rusty-patched bumblebee, Bombus affinis. Pollen samples were associated with a single Bombus affinis colony within a structure in Red Wing, Minnesota, USA, as previously described by Boone et al. (2022). This data release consists of two tab-delimited files: 1) sample.metadata.txt includes sample identifiers and accessions assigned by the National Center for Biotechnology Information (NCBI), the authoritative repository for publicly funded genetic data in the United States. These accessions can be used individually to obtain raw sequencing data or sample information at www.ncbi.nih.nlm.gov....


    map background search result map search result map Genetic detection of Lake Sinai Virus in honey bees (Apis mellifera) and other insects Voucher and metagenetic sequencing of DNA barcodes of wild-collected bees (Apoidea) from Iowa, USA Reduced representation sequencing and genotyping of Arizona Toads (Anaxyrus microscaphus) from the southwestern United States Whole genome sequencing of three North American large-bodied birds Amplicon sequencing of pollen foraged by Bombus affinis for compositional analysis Amplicon sequencing of pollen foraged by Bombus affinis for compositional analysis Voucher and metagenetic sequencing of DNA barcodes of wild-collected bees (Apoidea) from Iowa, USA Reduced representation sequencing and genotyping of Arizona Toads (Anaxyrus microscaphus) from the southwestern United States Genetic detection of Lake Sinai Virus in honey bees (Apis mellifera) and other insects Whole genome sequencing of three North American large-bodied birds