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Avian botulism toxicity is a common cause of death to water and shore birds that live near or migrate through Lake Michigan. The botulism neuro-toxin type E (bontE) gene is responsible for the production of botulinum neurotoxin type E. Quantitative Polymerase Chain Reaction (qPCR) was performed using a Step One Plus Thermocycler (Applied Biosystems) and protocol described in Getchell and others, 2011, Journal of Aquatic Animal Health. The assay was used to assess microbial community DNA obtained from environmental samples that were collected by Great Lakes Science Center and by National Park Service from 2011 to 2014 for the bontE gene. Samples were obtained by ponar grab or by divers and matrices collected...
This environmental dataset consists of 56 sediment, 24 Cladophora and 22 water samples collected from beaches at Glen Haven, Good Harbor, Platte Bay and Esch road beach, located within The Sleeping Bear Dunes National Lakeshore, Leelanau and Benzie counties, Michigan. The dataset includes matrix type, location, data, time and the qPCR reported value for each sample collected.
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