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Abstract Terminal restriction fragment length polymorphism (TRF or T-RFLP) analysis and 16S rDNA sequence analysis from clone libraries were used to examine cyanobacterial diversity in three types of predominant soil crusts in an arid grassland. Total DNA was extracted from cyanobacteria-, lichen-, or moss-dominated crusts that represent different successional stages in crust development, and which contribute different amounts of carbon and nitrogen into the ecosystem. Cyanobacterial 16S rRNA genes were amplified by PCR using cyanobacteria-specific 16S rDNA primers. Both TRF and clone sequence analyses indicated that the cyanobacterial crust type is dominated by strains of Microcoleus vaginatus, but also contains...
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Bravo Dome is a commercial natural CO2 field that supplies the gas to depleted oil fields for enhanced oil recovery. In order to understand the distribution of CO2 across the greater Bravo Dome region, including southeastern Colorado and northwestern New Mexico, and to assess the impacts of high CO2 concentrations on microbes in the subsurface, 7 samples were collected from sites associated with high natural CO2 concentrations. Four water samples were collected, two from wells producing within Bravo Dome, one from an oil and gas field north of Bravo Dome associated with high CO2 concentrations, the Oakdale field, and one sample from a shallow groundwater well on the Rafter K ranch that effervesces CO2. Three samples...
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Low biomass waters provide a unique challenge in the field of microbial ecology. It is difficult to determine, when biomass concentrations are extremely low, whether or not the sequencing data received are of good quality and representative of the waters sampled. Fifty-nine samples including 8 blanks were collected from a low biomass hydraulically fractured well producing from the Niobrara Shale in Colorado. At least 4 samples were collected by filtering the exact volume for each listed here: 1000 mL, 900 mL, 800 mL, 700 mL, 600 mL, 500 mL, 400 mL, 300 mL, 200 mL, 100 mL, 0 mL (blanks).
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These datasets contain microbial community data from groundwater samples collected at an in situ bioremediation site located at the former Naval Air Warfare Center (NAWC), West Trenton, NJ. DNA was extracted from groundwater samples collected from monitoring wells at the NAWC study site from July 2008 through July 2015 and analyzed for microbial community structure. Sample collection coincided with a groundwater bioremediation experiment investigating the microbial degradation of the contaminant trichloroethylene (TCE) prevalent in the targeted region of the aquifer. Nutrient addition and a microbial consortium, commercially developed to stimulate the degradation of TCE and TCE byproducts, was introduced to the...
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The Colorado Plateau is associated with several different commercial sources of natural CO2 and other non-hydrocarbon gases, such as noble gases. Twenty-five different hot springs, warm springs, cold springs, and/or rivers across the Utah region of the Colorado Plateau were sampled for microbial biomass. These different locations were associated with some concentration of dissolved or effervescing natural CO2 or other naturally-occurring non-hydrocarbon gases. Due to the possibility of different sources of the non-hydrocarbon gases across the region, multiple springs and rivers were sampled across a wide area of the state of Utah. Water samples were collected from non-stagnant regions of the sampled spring or river,...
    map background search result map search result map Microbial Communities Associated with Hot Springs and other CO2-rich Waters, Rocky Mountain Plateau Microbiology of the greater Bravo Dome region Low biomass microbiology samples collected from a hydraulically fractured well producing from the Niobrara Shale in Colorado Microbial community analyses of groundwater collected during an enhanced bioremediation experiment of trichlorethylene in a fractured rock aquifer, West Trenton, NJ (2008-2015) Microbial community analyses of groundwater collected during an enhanced bioremediation experiment of trichlorethylene in a fractured rock aquifer, West Trenton, NJ (2008-2015) Low biomass microbiology samples collected from a hydraulically fractured well producing from the Niobrara Shale in Colorado Microbiology of the greater Bravo Dome region Microbial Communities Associated with Hot Springs and other CO2-rich Waters, Rocky Mountain Plateau