Enzymes of Adenylate Metabolism and Their Role in Hibernation of the White-Tailed Prairie Dog, Cynomys leucurus

AMP deaminase (AMPD) and adenylate kinase (AK) were purified from skeletal muscle of the white-tailed prairie dog, Cynomus leucurus, and enzyme properties were assayed at temperatures characteristic of euthermia (37�C) and hibernation (5�C) to analyze their role in adenylate metabolism during hibernation. Total adenylates decreased in muscle of torpid individuals from 6.97 � 0.31 to 4.66 � 0.58 ?mol/g of wet weight due to a significant drop in ATP but ADP, AMP, IMP, and energy charge were unchanged. The affinity of prairie dog AMPD for AMP was not affected by temperature and did not differ from that of rabbit muscle AMPD, used for comparison. However, both prairie dog and rabbit AMPD showed much stronger inhibition...

Categories: Publication; Types: Citation, Journal Citation; Tags: AMP deaminase, Archives of Biochemistry and Biophysics, adenylate kinase, adenylate levels, energy charge, All tags... hibernation, metabolic rate depression, skeletal muscle, temperature-dependent kinetic properties, Fewer tags