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Although the variation in natural 15N abundance in plants and soils is well characterized, mechanisms controlling N isotopic composition of organic matter are still poorly understood. The primary goal of this study was to examine the role of NH3 volatilization from ungulate urine patches in determining 15N abundance in grassland plants and soil in Yellowstone National Park. We additionally used isotopic measurements to explore the pathways that plants in urine patches take up N. Plant, soil, and volatilized NH3d15N were measured on grassland plots for 10 days following the addition of simulated urine. Simulated urine increased 15N of roots and soil and reduced 15N of shoots. Soil enrichment was due to the volatilization...