Effect of SDS and Proteinase K on whole blood preserved in lysis buffer for Hawaiian Island avian malaria detection, 2005-2006

It is unclear whether DNA lysis buffers used for preservation of whole blood samples from Hawaiian forest birds cause denaturation and loss of antigen binding capability of antibody molecules. If their antigen binding capability is not affected, then the samples can be used in serological assays to provide an independent assessment of the accuracy of PCR (polymerase chain reaction) diagnostic tests for avian malaria. This data set reports raw absorbance measurements that were collected with a BioRad Model 3550 ELISA plate reader at a wavelength setting of 405 nm for different dilutions of blood from an infected Hawaii Amakihi that were preserved in PBS (phosphate buffered saline), TEN (Tris-Ethylenediaminetetraacetic...

Categories: Data; Tags: Chlorodrepanis virens, ELISA, Hakalau Forest National Wildlife Refuge, Hawaii, Hawaii Amakihi, All tags... Hawaiian Islands, Lysis Buffer, PCR, Plasmodium relictum, Western blot, avian malaria, enzyme linked immunosorbant assay, farming, immunoblot, polymerase chain reaction, Fewer tags

Derived ELISA Values for Hawaiian Island forest bird avian malaria detection using whole blood preserved in lysis buffer

It is unclear whether DNA lysis buffers used for preservation of whole blood samples from Hawaiian forest birds cause denaturation and loss of antigen binding capability of antibody molecules. If their antigen binding capability is not affected, then the samples can be used in serological assays to provide an independent assessment of the accuracy of PCR (polymerase chain reaction) diagnostic tests for avian malaria. This data set provides derived Percent ELISA (Enzyme linked Immunosorbant Assay) Values calculated from adjusted absorbance measurements that were collected with a BioRad Model 3550 ELISA plate reader at a wavelength setting of 405 nm.

Categories: Data; Tags: Chlorodrepanis virens, ELISA, Hakalau Forest National Wildlife Refuge, Hawaii, Hawaii Amakihi, All tags... Hawaiian Islands, Lysis Buffer, PCR, Plasmodium relictum, Western blot, avian malaria, biota, enzyme linked immunosorbant assay, immunoblot, polymerase chain reaction, Fewer tags

Raw absorbance values from serially diluted uninfected whole blood in different lysis buffers for Hawaiian Island forest bird avian malaria detection, 2005-2006

It is unclear whether DNA lysis buffers used for preservation of whole blood samples from Hawaiian forest birds cause denaturation and loss of antigen binding capability of antibody molecules. If their antigen binding capability is not affected, then the samples can be used in serological assays to provide an independent assessment of the accuracy of PCR (polymerase chain reaction) diagnostic tests for avian malaria. This data set provides raw absorbance measurements that were collected with a BioRad Model 3550 ELISA plate reader at a wavelength setting of 405 nm for different dilutions of blood from an uninfected Hawaii Amakihi. The blood was preserved in different lysis buffers or placed in phosphate buffered...

Categories: Data; Tags: Chlorodrepanis virens, ELISA, Hakalau Forest National Wildlife Refuge, Hawaii, Hawaii Amakihi, All tags... Hawaiian Islands, Lysis Buffer, PCR, Plasmodium relictum, Western blot, avian malaria, biota, enzyme linked immunosorbant assay, immunoblot, polymerase chain reaction, Fewer tags

Hawaii Island forest bird avian malaria detection using whole blood preserved in lysis buffer, 2005-2006

It is unclear whether DNA (Deoxyribonucleic Acid) lysis buffers used for preservation of whole blood samples from Hawaiian forest birds cause denaturation and loss of antigen binding capability of antibody molecules. If their antigen binding capability is not affected, then the samples can be used in serological assays to provide an independent assessment of the accuracy of PCR (polymerase chain reaction) diagnostic tests for avian malaria. This data release consists of three tabular datasets of raw absorbance measurements that were collected with a BioRad Model 3550 ELISA (Enzyme linked Immunosorbant Assay) plate reader at a wavelength setting of 405 nm from serial dilutions of whole blood that were preserved with...

Tags: Chlorodrepanis virens, ELISA, Hakalau Forest National Wildlife Refuge, Hawaii, Hawaii Amakihi, All tags... Hawaiian Islands, Lysis Buffer, PCR, Plasmodium relictum, USGS Science Data Catalog (SDC), Western blot, avian malaria, biota, enzyme linked immunosorbant assay, immunoblot, polymerase chain reaction, Fewer tags

Raw absorbance values for serially diluted whole blood preserved in different lysis buffers for Hawaiian Island forest bird avian malaria detection, 2005-2006

It is unclear whether DNA lysis buffers used for preservation of whole blood samples from Hawaiian forest birds cause denaturation and loss of antigen binding capability of antibody molecules. If their antigen binding capability is not affected, then the samples can be used in serological assays to provide an independent assessment of the accuracy of PCR (polymerase chain reaction) diagnostic tests for avian malaria. This data set provides raw absorbance measurements that were collected with a BioRad Model 3550 ELISA plate reader at a wavelength setting of 405 nm for different dilutions of blood from infected Hawaii Amakihi. The blood was preserved in different lysis buffers or placed in phosphate buffered saline...

Categories: Data; Tags: Chlorodrepanis virens, ELISA, Hakalau Forest National Wildlife Refuge, Hawaii, Hawaii Amakihi, All tags... Hawaiian Islands, Lysis Buffer, PCR, Plasmodium relictum, Western blot, avian malaria, biota, enzyme linked immunosorbant assay, immunoblot, polymerase chain reaction, Fewer tags