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This data release contains bioassay data from sediment toxicity tests conducted by the USGS Columbia Environmental Research Center (CERC) with 66 sediment samples collected from in and around the Upper Columbia River in the fall of 2013. Toxicity testing was conducted from fall 2013 through summer 2014 with the amphipod, Hyalella azteca, the midge Chironomus dilutus, and the mussel Lampsilis siliquoidea. Short-term toxicity endpoints (10-28 d) included survival, weight, and biomass of all test organisms. Long-term tests with amphipods (42 d) and midges (about 50 d) included reproduction endpoint. These data are intended to be used to characterize concentration-response relationships between metals concentrations...
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These data are comprised of histopathological analysis of liver and kidney tissues collected from small rodents collected in close proximity to brecchia pipe uranium mines in the Grand Canyon watershed.
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These data are comprised of measurements of elements (e.g., uranium, cobalt, nickel, copper, zinc, cadmium, lead, etc.), major anions (chloride, nitrite+nitrate as nitrogen, sulfate, etc.), dissolved organic carbon, and general water quality characteristics in Canyon Mine containment pond water samples collected in calendar year 2019.
Data consist of genotypes for pallid sturgeon and paddlefish broodstock and artificially-produced larvae at 19 sturgeon microsatellite loci and four paddlefish loci. Microsatellite genotypes allowed confirmation of homozygous sturgeon DNA and absence of paddlefish DNA in haploid gynogens. Data also consist of DNA yield estimates for haploid gynogens for proof of concept that haploid gynogens can provide sufficient DNA quantity for restriction site-associated DNA sequencing studies.
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Data were collected in association with the application of two physical barrier treatments, bentonite clay (a sealing agent) and expanding foam (an insulation sealant), in natural pond-burrows to suppress or kill the invasive red swamp crayfish (Procambarus clarkii). Data include burrow diameter and depth, crayfish sex and size, and determination of the treatment reaching the burrow chamber.
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These data are comprised of measurements of aluminum, iron, cobalt, nickel, copper, zinc, arsenic, selenium, molybdenum, silver, cadmium, thallium, lead, bismuth, thorium, uranium, and mercury in soil, vegetation, and small rodents. Gross alpha activity, gross beta activity, and radionuclide activities (isotopic U, isotopic Th) are also presented for vegetation and soil. Radioactivities for small rodents were previously presented at https://doi.org/10.5066/P9HARTQ4. Samples were collected at the Kanab North Mine (post-mining, pre-reclamation) in the Grand Canyon watershed.
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Data describe designed environmental DNA (eDNA) experiments for the estimation of eDNA shedding rates for the spectaclecase (Cumberlandia monodonta), mucket (Actinonaias ligamentina), and fatmucket (Lampsilis siliquoidea) from water samples collected in the laboratory under differing laboratory conditions. Shedding rates were tested under variable mussel biomass, diet, and temperature. Parameters described include descriptions of laboratory collected water samples that were tested to estimate concentration of spectaclecase, mucket or fatmucket DNA qPCR results, and associated quality assurance measurements.
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Using juvenile individuals of 3 species of carp (Family: Cyprinidae), we tested the potential for six amino acids to elicit an extracellular electro-olfactory response within the naris using gelatin-based electrodes. The amino acids L-alanine, L-arginine, L-aspartic acid, L-asparagine, L-glutamine, and L‑glutamic acid were tested on Grass Carp (Ctenopharyngodon idella), Bighead Carp (Hypophthalmichthys nobilis), and Silver Carp (H. molitrix) to provide a comparison among amino acids both within and among species. Each row in the data set represents exposure to a single amino acid. Each individual fish was exposed to up to four amino acids sequentially in a random order, representing a trial; each individual was...
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We investigated whether the length of time fire chemical weathers on a terrestrial substrate before mixing into aquatic environments alters the chemical’s toxicity when encountered by juvenile rainbow trout. We also looked at whether the type of substrate where the chemical is applied affects its toxicity to juvenile rainbow trout after mixing into water. Two fire chemicals (Phos Chek LC95A-R and Phos Chek MVP-Fx) were applied to four substrate media (low organic content soil, high organic content soil, duff, and gravel) and allowed to age on the substrate for 7, 14, 28 or 56 days. At the end of the assigned weathering period, 96-hour assays were conducted by adding water to the dosed substrate and stocking each...
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Data describe results of a set of laboratory experiments using a previously described quantitative thiaminase I activity assay to test the thiaminase activity of whole zebrafish homogenates. Parameters described include the thiaminase activity data, dilution factors, incubation time, and tissue weight assayed. Experiments were conducted at the USGS Columbia Environmental Research Center, Columbia, MO in 2006.
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Macrhybopsis reproduction and propagule traits were studied in the laboratory using two temperature regimes and three hormone treatments and which methods produced the most spawns. Only sicklefin chub (M. meeki) spawned successfully although sturgeon chub (M. gelida) released unfertilized eggs. All temperature and hormone treatments produced M. meeki spawns, but two treatments had similar success rates at 44 and 43%, consisting of a constant daily temperature with no hormone added, or daily temperature fluctuations with hormone added to the water. Spawns consisted of multiple successful demersal circular swimming spawning embraces interspersed with circular swims without embraces. The most spawns observed for one...
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This dataset describes the diameter, shape, settling velocity, and specific gravity of eggs from 9 female pallid sturgeon and 14 female shovelnose sturgeon as determined in laboratory studies. Pallid sturgeon and shovelnose sturgeon are believed to broadcast demersal eggs in the current, near the river bed over coarse substrate. It is hypothesized that eggs settle immediately following fertilization, but field conditions preclude direct observation. The dispersal of sturgeon eggs is affected by physical properties of the eggs and hydraulic conditions at the spawning location.
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These replicate data support the findings described in the publication, "A comparison of four pore water sampling methods for mixed metals and dissolved organic carbon, and implications for sediment toxicity evaluations" by Danielle Cleveland, William G. Brumbaugh, and Donald D. MacDonald (Environmental Toxicology and Chemistry 2017). The data represent comparison of four commonly-applied techniques for obtaining pore water samples for the quantification of nickel, copper, zinc, arsenic, cadmium, lead and dissolved organic carbon (DOC), including peepers, push points, centrifugation, and diffusive gradients in thin films (DGTs). The methods were evaluated at low and high concentrations of metals in three sediments...
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These data are comprised of measurements of aluminum, iron, cobalt, nickel, copper, zinc, arsenic, selenium, molybdenum, silver, cadmium, thallium, lead, bismuth, thorium, uranium, and mercury in soil, vegetation, and small rodents. Gross alpha activity, gross beta activity, and radionuclide activities (isotopic U, isotopic Th) are also presented for vegetation and soil. Radioactivities for small rodents were previously presented at https://doi.org/10.5066/P99GDFWB. The data also include histopathological analysis of liver tissues collected from small rodents. Samples were collected at the Kanab North Mine and Canyon Mine (post-mining, pre-reclamation) in the Grand Canyon watershed.
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A series of laboratory experiments were conducted to better understand the behavior of grass carp eggs and larvae in moving water in order to develop and implement new strategies for control and prediction of their dispersal and drift at early life stages. Settling velocity and density of a representative sample of eggs were estimated, and three trials of flume experiments with different flow conditions were conducted with live eggs in a temperature-controlled setting with a mobile sediment bed. In these trials, egg and larval stages were continuously analyzed over periods of 80 hours; and eggs and larvae interactions with the flow and sediment bed were monitored and characterized qualitatively and quantitatively....


map background search result map search result map Reproductive strategy, spawning induction, spawning temperatures and early life history of captive sicklefin chub Macrhybopsis meeki-Data Sediment bioassay toxicity data from the Upper Columbia River, Washington, 2013-2014 A comparison of four pore water sampling methods for mixed metals and dissolved organic carbon, and implications for sediment toxicity evaluations-Data Chemical analyses and histopathology of small rodents, vegetation, and soil collected from the Kanab North breccia pipe uranium mine in the Grand Canyon watershed Chemical analyses of small rodents, vegetation, and soil collected from the Kanab North breccia pipe uranium mine in the Grand Canyon watershed. Histopathological analyses of liver and kidney tissue from small rodents collected from breccia pipe uranium mines in the Grand Canyon watershed Survival and drifting patterns of grass carp eggs and larvae in response to interactions with flow and sediment in a laboratory flume Physical characteristics of pallid sturgeon and shovelnose sturgeon eggs Physical, Chemical, and Bioassay Data from the Study on Effects of Elevated Major Ions in Surface Water Contaminated by a Produced Water from Oil Production Survival and growth of juvenile freshwater mussels in a flow-through auto-feeding system Data for the assessment of the containment pond at Canyon Mine, Arizona, 2019-Water Chemical and biological data from acute and chronic nickel and zinc exposure bioassays to two sensitive freshwater benthic invertebrates Microsatellite genotypes and DNA yield of artificially produced larval pallid sturgeon, Scaphirhynchus albus Survival of grass carp and black carp gavaged with an oral dose of antimycin A in two carriers, corn oil and ethanol Thiaminase activity measurements in whole zebrafish Electro-olfactory responses of Grass Carp, Bighead Carp, and Silver Carp to the amino acids L-alanine, L-arginine, L-aspartic acid, L-asparagine, L-glutamine, and L‑glutamic acid Crayfish morphometric measurements, burrow attributes and occupancy in response to physical burrow barriers Alternative complement pathway assay data for adult largemouth bass exposed in outdoor ponds to 17alpha-ethinylestradiol or an estrone-atrazine mixture Water chemistry and biological data of Rainbow Trout following aquatic exposure to weathered wildland fire retardants after application to substrate Biomass experiments with Cumberlandia monodonta, Actinonaias ligamentina, and Lampsilis siliquoidea Crayfish morphometric measurements, burrow attributes and occupancy in response to physical burrow barriers Data for the assessment of the containment pond at Canyon Mine, Arizona, 2019-Water Survival and growth of juvenile freshwater mussels in a flow-through auto-feeding system Chemical and biological data from acute and chronic nickel and zinc exposure bioassays to two sensitive freshwater benthic invertebrates Thiaminase activity measurements in whole zebrafish Electro-olfactory responses of Grass Carp, Bighead Carp, and Silver Carp to the amino acids L-alanine, L-arginine, L-aspartic acid, L-asparagine, L-glutamine, and L‑glutamic acid Water chemistry and biological data of Rainbow Trout following aquatic exposure to weathered wildland fire retardants after application to substrate Biomass experiments with Cumberlandia monodonta, Actinonaias ligamentina, and Lampsilis siliquoidea Physical, Chemical, and Bioassay Data from the Study on Effects of Elevated Major Ions in Surface Water Contaminated by a Produced Water from Oil Production Alternative complement pathway assay data for adult largemouth bass exposed in outdoor ponds to 17alpha-ethinylestradiol or an estrone-atrazine mixture Survival and drifting patterns of grass carp eggs and larvae in response to interactions with flow and sediment in a laboratory flume Physical characteristics of pallid sturgeon and shovelnose sturgeon eggs Microsatellite genotypes and DNA yield of artificially produced larval pallid sturgeon, Scaphirhynchus albus Survival of grass carp and black carp gavaged with an oral dose of antimycin A in two carriers, corn oil and ethanol Chemical analyses and histopathology of small rodents, vegetation, and soil collected from the Kanab North breccia pipe uranium mine in the Grand Canyon watershed Chemical analyses of small rodents, vegetation, and soil collected from the Kanab North breccia pipe uranium mine in the Grand Canyon watershed. Histopathological analyses of liver and kidney tissue from small rodents collected from breccia pipe uranium mines in the Grand Canyon watershed Sediment bioassay toxicity data from the Upper Columbia River, Washington, 2013-2014