Filters: Tags: Columbia Environmental Research Center (X) > Types: Map Service (X)
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Macrhybopsis reproduction and propagule traits were studied in the laboratory using two temperature regimes and three hormone treatments and which methods produced the most spawns. Only sicklefin chub (M. meeki) spawned successfully although sturgeon chub (M. gelida) released unfertilized eggs. All temperature and hormone treatments produced M. meeki spawns, but two treatments had similar success rates at 44 and 43%, consisting of a constant daily temperature with no hormone added, or daily temperature fluctuations with hormone added to the water. Spawns consisted of multiple successful demersal circular swimming spawning embraces interspersed with circular swims without embraces. The most spawns observed for one...
We have adapted two commonly used human breast luciferase transactivation cell bioassays, the recently re-named estrogen agonist/antagonist screening VM7Luc4E2 cell bioassay (previously designated BG1Luc4E2) and the androgen/glucocorticoid screening MDA-kb2 cell bioassay, to 384-well formats for HTS of endocrine-active substances. This cost-saving adaptation includes a fast, accurate, and easy measurement of protein amount in each well via the fluorescamine assay (in the form of relative fluorescence units, RFU which are then converted to microgram protein using bovine serum albumin standards) with which to normalize luciferase activity (in the form of relative light units, RLU) of cell lysates without requiring...
Categories: Data;
Types: Map Service,
OGC WFS Layer,
OGC WMS Layer,
OGC WMS Service;
Tags: Columbia Environmental Research Center,
Missouri,
USGS Science Data Catalog (SDC),
bioassay,
cells,
The objectives of the present study were to develop methods for propagating western pearlshell (Margaritifera falcata) for laboratory toxicity testing and evaluate acute and chronic toxicity of chromium VI [Cr (VI)] to the pearlshell and a commonly tested mussel (fatmucket, Lampsilis siliquoidea at 20°C or in association with a co-stressor of elevated temperature (27°C), zinc (50 µg Zn/L), or nitrate (35 mg NO3/L). A commonly tested invertebrate (amphipod, Hyalella azteca) was also tested in chronic exposures.
Categories: Data;
Types: Map Service,
OGC WFS Layer,
OGC WMS Layer,
OGC WMS Service;
Tags: Acute Toxicity,
Chronic Toxicity,
Missouri,
Toxicity,
amphipods,
Invasive Asian carps established in the United States spawn in turbulent water of rivers and their eggs and early larvae develop while drifting in the current. The eggs are slightly denser than water and are held in suspension by water turbulence. The eggs are believed to perish if they settle before hatching. It is thus possible to use egg drift modeling to assess the capability of a river to support survival of Asian carp eggs. Data to populate such models include the physical properties of the assessed rivers, and information on egg size, density, and terminal fall velocity (sinking rates). Herein, we present the physical characteristics of the eggs as a function of post fertilization time. We recorded mean egg...
Categories: Data;
Types: Citation,
Map Service,
OGC WFS Layer,
OGC WMS Layer,
OGC WMS Service;
Tags: Bighead Carp,
Columbia Environmental Research Center,
Grass Carp,
Silver Carp,
USGS Science Data Catalog (SDC),
This dataset is a 30-meter resolution raster of estimated extent of subsurface tile drains, developed from tabular data of state-level estimates of agricultural land drained by tiles combined with geospatial cropland and soils in 12 Midwest States (SD, NE, KS, MN, IA, MO, WI, IL, MI, IN, OH, and KY). This dataset was created from the following four sources: 1) state-level acreages of agricultural "land drained by tiles" from the 2012 Census of Agriculture; 2) the extent of cultivated cropland from the National Land Cover Dataset (NLCD) 2011; 3) the extent of poorly and moderately drained soils from the State Soil Geographic Database (STATSGO) database Version 2; and 4) state administrative boundaries. The area of...
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