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We report the first simultaneous measurements of ?15N and ?13C of DNA extracted from surface soils. The isotopic composition of DNA differed significantly among nine different soils. The ?13C and ?15N of DNA was correlated with ?13C and ?15N of soil, respectively, suggesting that the isotopic composition of DNA is strongly influenced by the isotopic composition of soil organic matter. However, in all samples DNA was enriched in 13C relative to soil, indicating microorganisms fractionated C during assimilation or preferentially used 13C enriched substrates. Enrichment of DNA in 15N relative to soil was not consistently observed, but there were significant differences between ?15N of DNA and ?15N of soil for three...
Categories: Publication;
Types: Citation,
Journal Citation;
Tags: DNA,
Natural abundance ?13c,
Natural abundance ?15n,
Soil Biology and Biochemistry,
Soil microbial biomass
In 2015-2016, the Ohio Division of Wildlife’s undercover law enforcement purchased 1,200 grass carp (Ctenopharyngodon idella). Fish heads and eyeballs were sent overnight to U.S. Geological Survey Wetland and Aquatic Research Center for ploidy analysis. Field and laboratory standard operating procedures were established and followed. Fish lengths, fish weights, and eyeball weights were obtained from the U.S. Fish and Wildlife Service’s feral carp ploidy program for grass carp and black carp (Mylopharyngodon piceus) and the Ohio grass carp. Internal 2µm or 4µm bead standards were used in establishing nuclear sizes from Nile tilapia (Oreochromis niloticus), known diploid (n=20) and triploid (n=20) carp blood, as well...
This data set describes nuclear microsatellite genotypes derived from six autosomal loci (AgCA222, Age7.1JT, BV13, BV20, NVH206, NVH195-2) in Cooper's Hawks. Samples originated from blood samples collected from live trapped birds. Version History: First release: December 2020 Revised: February 2022 (ver. 2.0)
Categories: Data,
Data Release - Revised;
Tags: Accipiter cooperii,
AgCA222,
Age7.1JT,
Alleles,
Animals/Vertebrates,
This dataset contains stream-stage data for Santa Cruz River at Tumacácori Downstream Site (SC11). The pressure transducer was mounted to a metal post secured in the stream near the left bank. The data were recorded using non-vented Solinst Levelogger for the period of June 16, 2016 to June 24, 2016. The data were logged continuously at a 15-minute interval and manually downloaded. Data were then corrected with the atmospheric pressure collected from a Solinst Barologger located at SC14. The data was processed and analyzed for erroneous data.
Categories: Data;
Types: Citation;
Tags: DNA,
E. coli,
Escherichia coli,
Stage,
Tumacácori National Historical Park,
These are genetic data collected from replicated samples of 21 Enteroctopus (E. dofleini or a cryptic lineage) in Prince William Sound Alaska to evaluate tissue type, DNA extraction method, and time until analyses are completed on data reliability. Data collected from all samples include two microsatellites identified as possible lineage indicators, and nine microsatellite loci previously identified as polymorphic in both lineages. DNA sequence data from 528 bp of the octopine dehydrogenase (OCDE) gene were also collected.
Categories: Data;
Tags: Alaska,
Amino Acid Oxidoreductases,
Animals/Invertebrates,
Biodiversity,
Biodiversity,
This data set contains sampling information, allele sizes of 11 microsatellite loci, and Genbank accession numbers of ddRAD seq results for scoters (Melanitta sp.). Microsatellite data is provided for three North American species of scoter (black scoter, M. americana n = 61; white-winged scoter, M. deglandi, n = 208; surf scoter, M. perspicillata, n = 145) and their European congeners (common scoter, M. nigra, n = 19; velvet scoter, M. fusca, n = 20). Individuals with ddRAD results include 27 black scoters, 4 common scoters, 32 surf scoters, 28 white-winged scoters, and 4 velvet scoters.
We report the first simultaneous measurements of ?15N and ?13C of DNA extracted from surface soils. The isotopic composition of DNA differed significantly among nine different soils. The ?13C and ?15N of DNA was correlated with ?13C and ?15N of soil, respectively, suggesting that the isotopic composition of DNA is strongly influenced by the isotopic composition of soil organic matter. However, in all samples DNA was enriched in 13C relative to soil, indicating microorganisms fractionated C during assimilation or preferentially used 13C enriched substrates. Enrichment of DNA in 15N relative to soil was not consistently observed, but there were significant differences between ?15N of DNA and ?15N of soil for three...
Categories: Publication;
Types: Citation,
Journal Citation;
Tags: DNA,
Natural abundance ?13c,
Natural abundance ?15n,
Soil Biology and Biochemistry,
Soil microbial biomass
We characterized, at millimeter resolution, bacterial biomass, diversity, and vertical stratification of biological soil crusts in arid lands from the Colorado Plateau. Microscopic counts, extractable DNA, and plate counts of viable aerobic copiotrophs (VAC) revealed that the top centimeter of crusted soils contained atypically large bacterial populations, tenfold larger than those in uncrusted, deeper soils. The plate counts were not always consistent with more direct estimates of microbial biomass. Bacterial populations peaked at the immediate subsurface (1-2 mm) in light-appearing, young crusts, and at the surface (0-1 mm) in well-developed, dark crusts, which corresponds to the location of cyanobacterial populations....
Categories: Publication;
Types: Citation,
Journal Citation;
Tags: DNA,
Microbial Ecology,
RNA,
bacteria,
biomass,
This data set contains genetic information collected from eelgrass (Zostera marina) populations along the Pacific coast of North America from Alaska to Baha California. A total of 447 samples were collected comprising 401 unique individuals (genets) and 46 clones (ramets) from which 10 microsatellite DNA loci were obtained.
Categories: Data;
Types: Citation;
Tags: Alleles,
Angiosperms (Flowering Plants),
Aquatic Biology,
Aquatic ecosystems,
Bahia Magdelena,
This dataset contains stream-stage data for Santa Cruz River at Santa Gertrudis Lane (SC10). The pressure transducer was mounted to a metal post secured in the stream near the right bank. The data were recorded using non-vented Solinst Levelogger for the period of February 12, 2015 to December 31, 2016. The data were logged continuously at a 15-minute interval and manually downloaded. Data were then corrected with the atmospheric pressure collected from a Solinst Barologger located at SC14 and analyzed for erroneous data.
Categories: Data;
Types: Citation;
Tags: DNA,
E. coli,
Escherichia coli,
Stage,
Tumacácori National Historical Park,
This dataset contains stream-stage data for Santa Cruz River at Anza Trail Crossing (SC12). The pressure transducer was mounted to a metal post secured in the stream near the left bank. The data were recorded using non-vented Solinst Levelogger for the period of May 31, 2016 to December 31, 2016. The data were logged continuously at a 15-minute interval and manually downloaded. Data were then corrected with the atmospheric pressure collected from a Solinst Barologger located at SC14 and analyzed for erroneous data.
Categories: Data;
Types: Citation;
Tags: DNA,
E. coli,
Escherichia coli,
Stage,
Tumacácori National Historical Park,
Here we provide public access to six DNA sequence alignments and details on all specimens utilized in Smith and Johnson (2020).
Categories: Data;
Tags: DNA,
USGS Science Data Catalog (SDC),
biota,
conservation,
freshwater mussel,
The Utah sucker (Catostomus ardens) is endemic to the Bonneville Basin and the upper Snake River drainage in western North America, and is thought to hybridize with the federally endangered June sucker (Chasmistes liorus mictus) in Utah Lake (Bonneville Basin). Here we describe the discovery of a major subdivision in Utah suckers (4.5% mitochondrial sequence divergence) between the ancient Snake River drainage and the Bonneville Basin. This boundary has not previously been recognized in Utah suckers based on morphologic variation, but has been recently described in two endemic cyprinids in the region. Populations in valleys east of the Wasatch Mountains in Utah clustered with the Snake River populations, suggesting...
Categories: Publication;
Types: Citation,
Journal Citation;
Tags: Cypriniformes,
Cypriniformes: genetics,
DNA,
Molecular Ecology,
Utah,
This data set provides sample information, microsatellite genotype, and NCBI accession numbers for mitochondrial DNA sequences of peregrine falcons (Falco peregrinus) from North America and Russia. Version History: First release: January 2020 Revised: June 2022 (ver. 2.0)
Categories: Data,
Data Release - Revised;
Tags: Alaska,
Alberta,
Alleles,
Animals/Vertebrates,
Biodiversity,
This dataset provides cycle threshold (Ct) values achieved during qPCR for 6 sex-linked genes, and two autosomal genes, for individuals from 60 species and across 13 orders of birds.
These data are comprised of two tables, one table containing wolverine (Gulo gulo) sample and genetic information for 20 microsatellite loci (Gg10-1, Gg25, Gg37-2, Gg42-1, Gg192-1, Gg443, Gg452, Gg454, Gg465, Gg471, Gg473, Gg-3-1, Gg-4, Gg-7-1, Ggu_216-1, Lut604, Ma-3-1, Mvis075, Tt-1, and Tt-4) and genetic sex determination results from North America and one Russian wolverine. All samples were obtained from collections at the University of Alaska Museum of the North or the University of New Mexico’s Museum of Southwestern Biology. The second table provides complete primer information for the genetic markers since several were redesigned from their originally-published sequences for this study.
Here we provide public access to three DNA sequence alignments (COI, ND1, ITS1) and details on all specimens utilized in Keogh et al. (in review).
This dataset contains stream-stage data for Nogales Wash at Ruby Road (NW8). The pressure transducer was mounted to a metal post secured in the stream near the left bank. The data were recorded using non-vented Solinst Levelogger for the period of February 12, 2015 to December 31, 2016. The data were logged continuously at a 15-minute interval and manually downloaded. Data were then corrected with the atmospheric pressure collected from a Solinst Barologger located at SC14 and analyzed for erroneous data.
Categories: Data;
Types: Citation;
Tags: DNA,
E. coli,
Escherichia coli,
Stage,
Tumacácori National Historical Park,
This dataset contains E. coli data collected at the Outfall of the Nogales International Wastewater Treatment Plant (NIWTP; SC6, 312724110580501) by U.S. International Boundary and Water Commission (IBWC) personnel. The IBWC collects discrete samples at the Outfall of the NIWTP daily or weekly by dipping a 100 mL sterilized IDEXX bottle in the centroid of flow. Samples are kept chilled and shipped to Turner Laboratories Inc. or Legend Technical Services Inc. Both laboratories use standard method 9223B for E. coli analysis, which uses the Colilert™-24 (Colilert) and Quanti-Tray®/2000 (Quanti-Tray) system. The holding time specified for wastewater is 30 hours. The dataset is a compilation of multiple paper records....
Categories: Data;
Types: Citation;
Tags: DNA,
E. coli,
Escherichia coli,
Stage,
Tumacácori National Historical Park,
This data set describes nuclear microsatellite genotypes derived from eight autosomal loci (Aph02, Aph11, Aalµ1, Sfiµ4, Smo4, Smo7, Smo12, and Sfiµ8) and accession numbers for double digest restriction-site associated DNA (ddRAD) sequences.
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