Filters: Tags: FEMS Microbiology Ecology (X) > partyWithName: Cheryl R. Kuske (X)
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Abstract Terminal restriction fragment length polymorphism (TRF or T-RFLP) analysis and 16S rDNA sequence analysis from clone libraries were used to examine cyanobacterial diversity in three types of predominant soil crusts in an arid grassland. Total DNA was extracted from cyanobacteria-, lichen-, or moss-dominated crusts that represent different successional stages in crust development, and which contribute different amounts of carbon and nitrogen into the ecosystem. Cyanobacterial 16S rRNA genes were amplified by PCR using cyanobacteria-specific 16S rDNA primers. Both TRF and clone sequence analyses indicated that the cyanobacterial crust type is dominated by strains of Microcoleus vaginatus, but also contains...
Categories: Publication;
Types: Citation,
Journal Citation;
Tags: 16S rRNA sequencing,
FEMS Microbiology Ecology,
arid land soil,
biological soil crust,
cyanobacterial diversity,
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