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The data document the results of several microbe bioassays performed by the USGS on Phragmites australis plants, including those performed on mature leaves, seedlings, and dead leaf tissues exploration of the literature to find accounts of microbes associated with Phragmites worldwide. For the bioassays, we prepared 162 pure cultures isolated from Phragmites plants in North America along the east coast, Florida, the Gulf of Mexico, and the Great Lakes area, 125 of which were from a previous study, and 38 represent new collections. The DNA sequences used to identify the 37 new collections are included. Microbes were isolated from plants collected from 2015-2018. We performed assays using both North American plant...
Soil organic matter (SOM) biomarker methods were utilized in this study to investigate the responses of fungi and bacteria to freeze–thaw cycles (FTCs) and to examine freeze–thaw-induced changes in SOM composition and substrate availability. Unamended, grass-amended, and lignin-amended soil samples were subject to 10 laboratory FTCs. Three SOM fractions (free lipids, bound lipids, and lignin-derived phenols) with distinct composition, stability and source were examined with chemolysis and biomarker Gas Chromatography/Mass Spectrometry methods and the soil microbial community composition was monitored by phospholipid fatty acid (PLFA) analysis. Soil microbial respiration was also measured before and during freezing...
In arid ecosystems, abiotic processes facilitate the physical and chemical degradation of plant litter to the extent that decomposition models that use climatic and litter composition variables as surrogates for microbial activity are not predictive. The purpose of this study was to estimate the potential contribution of photodegradation to the decomposition of plant litters that varies in architecture and chemical composition. Litter of Pinus edulis, Juniperus monosperma and Populus deltoides were exposed to ambient and attenuated sunlight, with and without supplemental water additions, at a riparian forest site along the Middle Rio Grande (New Mexico, USA). Mass loss, elemental composition, and microbial extracellular...
Soil organic matter (SOM) biomarker methods were utilized in this study to investigate the responses of fungi and bacteria to freeze?thaw cycles (FTCs) and to examine freeze?thaw-induced changes in SOM composition and substrate availability. Unamended, grass-amended, and lignin-amended soil samples were subject to 10 laboratory FTCs. Three SOM fractions (free lipids, bound lipids, and lignin-derived phenols) with distinct composition, stability and source were examined with chemolysis and biomarker Gas Chromatography/Mass Spectrometry methods and the soil microbial community composition was monitored by phospholipid fatty acid (PLFA) analysis. Soil microbial respiration was also measured before and during freezing...
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The Sevilleta LTER supports a long-term, integrated, interdisciplinary research program addressing key hypotheses on pattern and process in aridland ecosystems. Sevilleta LTER research includes studies in desert grassland and shrubland communities, and riparian and mountain forests emphasizing pulse driven processes in space and time. Key drivers (e.g., climate, fire, water, resource availability) govern dynamics in each landscape component. Our focus on how biotic and abiotic drivers affect spatial and temporal dynamics of aridland ecosystems allows us to conduct long-term research that addresses important basic theories and yet has significant relevance to regional, national and international priorities. The...
Warming-induced release of CO2 from the large carbon (C) stores in arctic soils could accelerate climate change. However, declines in the response of soil respiration to warming in long-term experiments suggest that microbial activity acclimates to temperature, greatly reducing the potential for enhanced C losses. As reduced respiration rates with time could be equally caused by substrate depletion, evidence for thermal acclimation remains controversial. To overcome this problem, we carried out a cooling experiment with soils from arctic Sweden. If acclimation causes the reduction in soil respiration observed after experimental warming, then it should subsequently lead to an increase in respiration rates after cooling....
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To determine how native and non-native lineages of Phragmites australis affect and respond to soil bacteria, fungi and oomycetes, we collected live rhizomes, seeds and soil from native and non-native lineages of Phragmites from 10 sites within Michigan and Ohio, USA. We propagated these field-collected samples to carry out a reciprocal-transplant plant-soil feedback experiment with multiple microbial inhibition treatments. Specifically, we investigated how each Phragmites lineage grew in soils pre-conditioned by each lineage and soils that had been pre-sterilized. Plant biomass was the main response variable collected to determine responses to microbial soil conditioning. We also used DNA meta-barcoding to identify...
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To determine the differences in soil microbial community composition between native and non-native lineages of Phragmites, we sampled soils from eight sites in the Great Lakes basin where populations of native and non-native Phragmites co-occurred. In addition, we included samples of soils from 27 populations of Phragmites across the Gulf of Mexico and Atlantic Coasts of the US. Samples were collected between July 2015 and September 2017. At each site in the Great Lakes, we sampled rhizosphere and bulk soil surrounding one ramet of each lineage. Samples from Atlantic and Gulf coasts were collected by homogenizing rhizosphere soils from multiple ramets of one population within a single lineage. DNA was extracted...
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Description of Work The invasive form of Phragmites australis (common reed) is a well-established pest in many parts of the Great Lakes and the Atlantic, Gulf, and Pacific coasts, including designated Areas of Concern. New innovative control options that sustainably target the competitive advantage often enjoyed by Phragmites and other invasive plants will contribute to a broad Integrated Pest Management (IPM) strategy. This project targets the microorganisms that may help Phragmites spread and will employ a molecular genetic approach to silence the genes in Phragmites that give it a competitive edge over many native plants. This project helped build and will continue to be closely aligned with the Great Lakes Phragmites...
Soil organic matter (SOM) biomarker methods were utilized in this study to investigate the responses of fungi and bacteria to freeze?thaw cycles (FTCs) and to examine freeze?thaw-induced changes in SOM composition and substrate availability. Unamended, grass-amended, and lignin-amended soil samples were subject to 10 laboratory FTCs. Three SOM fractions (free lipids, bound lipids, and lignin-derived phenols) with distinct composition, stability and source were examined with chemolysis and biomarker Gas Chromatography/Mass Spectrometry methods and the soil microbial community composition was monitored by phospholipid fatty acid (PLFA) analysis. Soil microbial respiration was also measured before and during freezing...
Understanding of the influence of global warming has been limited by a paucity of experiments. Taking advantage of the largest, longest-running experimental warming of a forest, we convened dozens of scientists from across the world to collect data to study and understand how bacteria, fungi, herbivores, plant pathogens, insects and a diversity of other groups respond to warming. We found that warming had a significant impact on ecosystems at both a site in North Carolina, as well as a more northern site in Massachusetts. The types of effects, however, differed between the north and south; they also differed as a function of the organisms considered. While warming affected all levels of organization, it had the...
Many Army land managers find stabilization, rehabilitation, and revegetation extremely difficult on disturbed lands in arid and semi-arid regions. Blackbrush, Coleogyne ramosissima, occurs as a landscape dominant in the ecotonal region between hot and cold deserts of the western United States. Soils in this region are often characterized by well-developed microbiotic soil crusts. Revegetation efforts using blackbrush have met with limited success, prompting speculation on possible interactions with soil microorganisms, including mycorrhizal fungi. A series of experiments conducted from 1993 to 1997 tested the effect of inoculation with arbuscular mycorrhizal fungi on the growth of young blackbrush seedlings under...
* 1 Soils are one of the last great frontiers for biodiversity research and are home to an extraordinary range of microbial and animal groups. Biological activities in soils drive many of the key ecosystem processes that govern the global system, especially in the cycling of elements such as carbon, nitrogen and phosphorus. * 2 We cannot currently make firm statements about the scale of biodiversity in soils, or about the roles played by soil organisms in the transformations of organic materials that underlie those cycles. The recent UK Soil Biodiversity Programme (SBP) has brought a unique concentration of researchers to bear on a single soil in Scotland, and has generated a large amount of data concerning biodiversity,...
Bromus tectorum is an exotic annual grass that currently dominates many western U.S. semi-arid ecosystems, and the effects of this grass on ecosystems in general, and soil biota specifically, are unknown. Bromus recently invaded two ungrazed and un-burned perennial bunchgrass communities in southeastern Utah. This study compared the soil food-web structure of the two native grassland associations (Stipa [S] and Hilaria [H]), with and without the presence of Bromus. Perennial grass and total vascular-plant cover were higher in S than in H plots, while quantities of ground litter were similar. Distribution of live and dead plant material was highly clumped in S and fairly homogenous in H. Soil food-web structure was...
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Bromus tectorum is an exotic annual grass that currently dominates many western U.S. semi-arid ecosystems, and the effects of this grass on ecosystems in general, and soil biota specifically, are unknown. Bromus recently invaded two ungrazed and un-burned perennial bunchgrass communities in southeastern Utah. This study compared the soil food-web structure of the two native grassland associations (Stipa [S] and Hilaria [H]), with and without the presence of Bromus. Perennial grass and total vascular-plant cover were higher in S than in H plots, while quantities of ground litter were similar. Distribution of live and dead plant material was highly clumped in S and fairly homogenous in H. Soil food-web structure was...
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This data record contains arbuscular mycorrhizal fungi (AMF) operational taxonomic unit (OTU) occurrences with native prairie plant species roots collected from paired remnant and reconstructed prairies, as well as soil physical and chemical property data from these field sites in Minnesota and Iowa.
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Reported here are the fluorescence data points recorded from CRISPR/Cas12a biosensor assays to detect the presence or absence of DNA from Pseudogymnoascus destructans (Pd), the causative fungal agent of white-nose syndrome in bats, from artificial controls, dermal swab, and guano samples. Ct values from qPCR experiments are also provided in a table. Furthermore, we also provide the GenBank accession numbers used to develop the aforementioned CRISPR/Cas12a biosensor assay for Pd DNA detection.
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Recent estimates of the magnitude of species’ range shifts, extinctions, and changes in ecosystem processes resulting from climatic change are alarming. Estimates of extinction rates and population losses attributable to climatic change are often based on studies of modeled biogeographic distributions, which rely on simple relationships between present-day distributions of species and climate variables to estimate distributions of species under future climate. Hundreds of papers using distribution models - based almost exclusively on relationships between plants and climate - have been published. The predictions of these models undergird conservation plans, are used to forecast the magnitude of future extinctions,...
Dynamics of nutrient exchange between floodplains and rivers have been altered by changes in flow management and proliferation of nonnative plants. We tested the hypothesis that the nonnative, actinorhizal tree, Russian olive (Elaeagnus angustifolia), alters dynamics of leaf litter decomposition compared to native cottonwood (Populus deltoides ssp. wislizeni) along the Rio Grande, a river with a modified flow regime, in central New Mexico (U.S.A.). Leaf litter was placed in the river channel and the surface and subsurface horizons of forest soil at seven riparian sites that differed in their hydrologic connection to the river. All sites had a cottonwood canopy with a Russian olive-dominated understory. Mass loss...
    map background search result map search result map Soil Biota in an Ungrazed Grassland: Response to Annual Grass (Bromus tectorum) Invasion Invasive Phragmites: Prevention, Monitoring, and Control Strategies in an Integrated Pest Management Framework Sevilleta LTER Forests of the Future: Integrated Assessment of Climate Change and Ecosystem Diversity The effects of North American fungi and bacteria on Phragmites australis leaves 2017-2019, with comparisons to the global Phragmites microbiome Soil microbes surrounding native and non-native Phragmites australis in the Great Lakes and East Coast of the United States (2015-2017 survey) (ver. 1.1, December 2020) Phragmites australis responses to and microbial community composition of greenhouse soils (2018-2019 experiment) Arbuscular mycorrhizal fungi in remnant and reconstructed prairies in Minnesota and Iowa, 2019 (ver. 2.0, April 2022) Data from the development and evaluation of a CRISPR/Cas12a-based biosensor for the detection of DNA from Pseudogymnoascus destructans using samples collected from various species of bats across the United States, from 2018 to 2023 Forests of the Future: Integrated Assessment of Climate Change and Ecosystem Diversity Sevilleta LTER Phragmites australis responses to and microbial community composition of greenhouse soils (2018-2019 experiment) Arbuscular mycorrhizal fungi in remnant and reconstructed prairies in Minnesota and Iowa, 2019 (ver. 2.0, April 2022) Invasive Phragmites: Prevention, Monitoring, and Control Strategies in an Integrated Pest Management Framework Soil microbes surrounding native and non-native Phragmites australis in the Great Lakes and East Coast of the United States (2015-2017 survey) (ver. 1.1, December 2020) Data from the development and evaluation of a CRISPR/Cas12a-based biosensor for the detection of DNA from Pseudogymnoascus destructans using samples collected from various species of bats across the United States, from 2018 to 2023 The effects of North American fungi and bacteria on Phragmites australis leaves 2017-2019, with comparisons to the global Phragmites microbiome