Filters: Tags: Genetics (X)
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We collected environmental DNA (eDNA) data from the Elwha River, home to the world’s largest dam removal project, to track the spatial and temporal patterns of species responses following dam removal. In total, we collected data for 11 different fish taxa, sampled at 25 sites ranging across 56 river kilometers in a wilderness river for 4 years following dam removal. We show that eDNA can effectively be used to determine whether fish have recolonized past former dams, and in some cases determine the spatial extent of that recolonization.
This dataset provides spatial predictions of the pooled-SDM residuals from a multiscale geographically weighted regression model (MGWR) and the resulting local R2 values for individuals in the subregion encompassing the genetic sampling locations used by Edwards et al. (2015). This region offered an opportunity to explore habitat selection across the ecotone between the Mojave and Sonoran deserts and the secondary contact zone between G. agassizii and G. morafkai, and is referred to as the focal study area. The raster layers contained here accompany the manuscript Inman et al. 2019 and were used to identify multivariate clusters and map them back to geographic space. Inman et al. 2019. Local niche differences predict...
We generated multilocus DNA sequence data and traditional morphometric measurements to evaluate species boundaries in Fusconaia mitchelli. We sequenced three loci: the protein-coding mitochondrial DNA genes cytochrome oxidase subunit 1 and NADH dehydrogenase 1, and the nuclear ribosomal internal transcribed spacer 1. We also took three measurements, maximum length, maximum width, and maximum height, of mussel shells for morphometric analysis.
Here we provide public access to three DNA sequence alignments (COI, ND1, ITS1) and details on all specimens utilized in Keogh et al. (in review).
Red lionfish (Pterois volitans) have become a successful invasive predator across the Northwestern Atlantic, Caribbean, and Gulf of Mexico (GoM). Previous investigations have identified the southeast coast of Florida as the original site of introduction, but no region-wide genetic study has directly addressed the question of introduction location(s). This dataset includes previously unpublished red lionfish samples (n = 237) from six locations: The Bahamas, Florida Keys, Northwest Florida, North Carolina, Panama, and Southeast Florida. Sequences archived in NCBI from other locations in the Northern Region, Caribbean, and Gulf of Mexico basins were used in the analyses (N = 1558). Previously published sequences were...
These data show grass crop and model species response to toxic chemicals (Arsenic (As)) and humic acids. Experiments were performed by collaboration between the U.S. Geological Survey, Rutgers University, and Rey Juan Carlos University. A series of individual experiments investigated beneficial effects of endophytic bacteria on grass crop growth and resilience to known plant toxicity.
Categories: Data;
Tags: Ecology,
Genetics,
North America,
USGS Science Data Catalog (SDC),
bacteria,
The U.S. Geological Survey provides a wide range of scientific information to an even wider group of stakeholders. Understanding what capacities are needed and if and or where these capacities exist across the USGS landscape is critical in moving science to the next level of use, implementation, and visualization. The concept behind the groups organized to conduct and interpret the survey that collected these data took advantage of the USGS’s position as a science organization with expertise spanning a wide range of science disciplines, stakeholders, and responsibilities. A survey was conducted of USGS employees (Sep 20-Nov 20) to get a current sample of the capacities that exist across the USGS.
Categories: Data;
Types: Map Service,
OGC WFS Layer,
OGC WMS Layer,
OGC WMS Service;
Tags: Aquatic Biology,
Botany,
Climatology,
Ecology,
Economic Geology,
This data release contains molecular diagnostic results and molecular detection of the myxozoan parasite Tetracapsuloides byrosalmonae in fish tissue and environmental DNA samples collected from the inter-mountain West, USA.
Introduction and translocation programs require thoughtful planning to increase the likelihood of success and to understand the benefits, risks, and constraints of such programs. A risk assessment was completed for bull trout introduction into the Sullivan Lake and Harvey Creek watershed, northeastern Washington. The risk assessment was designed to evaluate potential risks to resident fish species, to bull trout introduced into Sullivan Lake, and to bull trout donor source populations. The risk assessment describes the potential risks associated with pathogens, genetics, and ecological interactions. Literature reviews were completed for fish species composition and abundance in Sullivan Lake watershed to assess...
Categories: Data;
Types: Map Service,
OGC WFS Layer,
OGC WMS Layer,
OGC WMS Service;
Tags: Aquatic Biology,
British Columbia,
Ecology,
Genetics,
Idaho,
Here we provide public access to UCE DNA sequences for all genetic samples in Byerly et al. 2022b.
Categories: Data;
Tags: Azores,
Caribbean Islands,
DNA sequencing,
Florida Bay-Florida Keys,
Genetics,
Biological indicator taxa have long been used for integrative assessments of water quality, particularly benthic invertebrate groups such as arthropods. While standardized protocols have been developed to calculate 'biological index' scores based on the abundances of these taxa, such systems are challenging to implement at large scales due to the sampling effort required, taxonomic expertise needed, and the need for repeated sampling to reliably discriminate sites. Many of the same taxa detected by traditional surveys can also be detected by genetic analysis of environmental DNA (eDNA), potentially allowing for an alternative formulation of biological indexes that might be faster and more economical to produce....
This project used species distribution modeling, population genetics, and geospatial analysis of historical vs. modern vertebrate populations to identify climate change refugia and population connectivity across the Sierra Nevada. It is hypothesized that climate change refugia will increase persistence and stability of populations and, as a result, maintain higher genetic diversity. This work helps managers assess the need to include connectivity and refugia in climate change adaptation strategies. Results help Sierra Nevada land managers allocate limited resources, aid future scenario assessment at landscape scales, and develop a performance measure for assessing resilience.
Categories: Data,
Project;
Tags: 2011,
2013,
CA,
California Landscape Conservation Cooperative,
Conservation Design,
Latitudes and longitudes for 45 sites samples for flathead catfish population genetic analyses.
Taxonomic composition of environmental DNA acquired by filtration from the St. Regis River, New York
Environmental DNA (eDNA) surveys have become important tools for monitoring aquatic biodiversity. Barcode sequencing of eDNA generates community profiles that, while potentially biased in both capture and amplification, can nonetheless yield high information content per unit cost. While factors affecting eDNA capture and amplification have been heavily studied, watershed-scale assessments of fish communities and our confidence in such have been less frequent. We performed an initial watershed-scale characterization of fish eDNA using rapid, low-volume filtering with replicate and control samples scaled for a single Illumina MiSeq flow cell, using the mitochondrial 12S ribosomal RNA locus for taxonomic profiling....
The data set consists of 12 input data rasters that cover San Diego County, California. These input rasters represent criteria used in a Pareto ranking algorithm in the manuscript. These include three rasters related to fire threats, three rasters related to habitat fragmentation threats, four rasters related to species biodiversity, and two rasters related to genetic biodiversity. (see the PLOS ONE paper for details). These data support the following publication: Tracey JA, Rochester CJ, Hathaway SA, Preston KL, Syphard AD, Vandergast AG, et al. (2018) Prioritizing conserved areas threatened by wildfire and fragmentation for monitoring and management. PLoS ONE 13(9): e0200203. https://doi.org/10.1371/journal.pone.0200203
This dataset includes microsatellite genotypes for 8,454 brook trout from 188 wild Midwestern populations and 26 hatchery strains of both Midwest and eastern (Atlantic seaboard) origin. Each individual was genotyped at either 5 or 7 loci.
Categories: Data;
Tags: Aquatic Biology,
Genetics,
Genetics,
Midwest,
USGS Science Data Catalog (SDC),
These data were compiled to investigate the evolutionary history of Graham's beardtongue (Penstemon grahamii). Objective(s) of our study were to determine the evolutionary history of P. grahamii, including ancestral population sizes, the history of population divergences, and historical connectivity. In addition, we characterized population structure, genetic diversity summary statistics, and landscape factors influencing differentiation. These data represent anonymous loci sequenced from throughout the P. grahamii genome (specifically, .vcf and .structure files). Data in these files were manipulated to represent site frequency spectra between population pairs (.data files). These data were collected in 2019 from...
To determine how native and non-native lineages of Phragmites australis affect and respond to soil bacteria, fungi and oomycetes, we collected live rhizomes, seeds and soil from native and non-native lineages of Phragmites from 10 sites within Michigan and Ohio, USA. We propagated these field-collected samples to carry out a reciprocal-transplant plant-soil feedback experiment with multiple microbial inhibition treatments. Specifically, we investigated how each Phragmites lineage grew in soils pre-conditioned by each lineage and soils that had been pre-sterilized. Plant biomass was the main response variable collected to determine responses to microbial soil conditioning. We also used DNA meta-barcoding to identify...
This dataset contains the physical collection information (e.g., sample location, date, gear type) and microsatellite DNA genotype of egg and larval Lake Sturgeon collected in the St. Clair and Detroit rivers in 2015 and 2016. Individuals were genotyped for 18 microsatellite loci (13 disomic and 5 polysomic). Alleles (base pair sizes) were recorded as presence absence scores (1:present, 2:absent, 0:missing data) for all previously observed alleles. Thus individual genotypes were recorded as pseudo diploid dominant phenotypes resulting in individual vectors of length n=205 for each genotyped egg or larval individual.
Categories: Data;
Tags: Acipenser,
Acipenser fulvescens,
Acipenseridae,
Anthropogenic,
Anthropogenic Rock,
We tested the utility of a protocol using genetic markers that previously proved successful to identify the sex of Vespertilionid bats on tissues collected from live bats and carcasses of varying age from the Hawaiian hoary bat (Lasiurus semotus). This molecular method is based on genes unique to X and Y chromosomes in mammals and previously was used successfully on North American hoary bats (L. cinereus). Using genotyping, we determined the sex of 123 of 125 Hawaiian hoary bat tissue samples from carcasses. This dataset includes all 73 samples from the original data release, plus 58 additional samples. NOTE: While previous versions are available from the author, all the records in previous versions can be found...
Categories: Data,
Data Release - Revised;
Tags: Hawaii,
Hawaiian Islands,
Hawaiian hoary bat,
Maui,
Oahu,
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