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The field of soil ecology has relatively few fundamental unifying principles that can be used to explain and predict patterns and processes in belowground ecosystems. Here we propose that a first step towards developing a more comprehensive set of unifying principles in soil ecology is to identify and understand the characteristics shared by a wide range of soils, the common mechanisms driving soil biogeochemical processes, and the biogeochemical constraints imposed on soil biota regardless of soil type. Very often, soil ecologists focus on the differences between soils when, in fact, many soils share a common set of ecological mechanisms that govern biogeochemical processes. Here we explore evidence for the existence...
Our objective is to improve the scientific understanding of the modes, rates, and mechanisms of carbon stabilization and losses in soils from Alaska, California, and other Western states. We focus on the biophysical and microbial mechanisms that drive carbon gains and losses, and to use our data to improve models of soil carbon cycling. This catalog supports research from several projects focused on soil carbon cycling. It encompasses multiple types of datasets including environmental, ecological, biological, isotopic, mineralogical, genomic, flux, and modeled data from water, vegetation, soil, and atmospheric matrices. The catalog will be available online and to the public. Therefore, publication of data through...
Because microorganisms are sensitive to temperature, ongoing global warming is predicted to influence microbial community structure and function. We used large-scale warming experiments established at two sites near the northern and southern boundaries of US eastern deciduous forests to explore how microbial communities and their function respond to warming at sites with differing climatic regimes. Soil microbial community structure and function responded to warming at the southern but not the northern site. However, changes in microbial community structure and function at the southern site did not result in changes in cellulose decomposition rates. While most global change models rest on the assumption that taxa...
The research field of animal and plant symbioses is advancing from studying interactions between two species to whole communities of associates. High-throughput sequencing of microbial communities supports multiplexed sampling for statistically robust tests of hypotheses about symbiotic associations. We focus on ambrosia beetles, the increasingly damaging insects primarily associated with fungal symbionts, which have also been reported to support bacteria. To analyze the diversity, composition, and specificity of the beetles’ prokaryotic associates, we combine global sampling, insect anatomy, 454 sequencing of bacterial rDNA, and multivariate statistics to analyze prokaryotic communities in ambrosia beetle mycangia,...
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This data release contains soil and vegetation data collected along a post glacial chronosequence associated with populations of oriental bittersweet (Celastrus orbiculatus) at the Indiana Dunes National Park. The post glacial chronosequence includes sand dunes and moraines, representing a successional gradient from woodland transition, to oak savanna, woodland and forest, to oak hickory forests on the Lake Border Moraine. Soil samples were collected from August 24, 2017 to September 1, 2017; vegetation cover data (percent cover by species) were collected from 1/4-meter squared plots (0.5 by 0.5 m) where the soil samples were taken. DNA extracted from soil and rhizosphere samples were analyzed for bacterial communities...
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The data being released were part of a project funded by the United States Environmental Protection Agency (USEPA). This study sought to identify the planktonic communities (cyanobacteria, eukaryotic algae) potentially contributing to eutrophication within the Grand Calumet River Area of Concern (AOC) in northern Indiana along the southern shore of Lake Michigan. In 2021, triplicate water samples were collected from five locations during three events, 4/19/21 and 4/20/21, 7/7/21, and 9/15/21. Water samples were processed and planktonic communities were determined by a DNA-based technology (algal metabarcoding). Sampling locations: 1. Columbia Avenue, Hammond, IN 2. Lake George drainage ditch, Hammond, IN 3. Indiana...
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Site, field, and soil data collected from 14 sites along a chronosequence of wetland submergence on 15 – 17 October 2019 in a Louisiana salt marsh in Barataria Basin, part of the Mississippi River Deltaic Plain, along the northern Gulf of Mexico coast.
The data being released were part of a project funded by the Great Lakes Restoration Initiative (GLRI). This study sought to examine the influence of filter pore size (5.0 µm pre and 0.22 µm final filtration) on microbial communities and source-specific microbial source tracking (MST) markers at three locations along southern Lake Michigan: Racine, WI; Chicago, IL; and East Chicago, IN; between 2015 and 2017. In 2015, triplicate water samples were collected during three events, in 2016 individual water samples were collected during three events, and in 2017, individual water samples were collected one day a week for ten weeks between June and August. Samples were collected from twelve locations, two river, two river...
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This soil and vegetation data were collected along a successional gradient at the Indiana Dunes National Park. Soil samples were processed for standard soil chemistry, texture, and nutrients. DNA extracted from soil samples were analyzed for bacterial communities using 16S rRNA amplicon sequencing technique to understand the composition and distribution of these communities along the successional gradient. Soils samples were collected in 2015 and 2017. Vegetation data were collected only in 2017, as the vegetation was dormant in 2015. We collected the data to examine the relationships among soils, vegetation, and composition of the microbial communities along a dunal chronosequence. A dunal chronosequence is a series...
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The data associated with the following data release were collected between 2016 and 2017 at three locations on Lake Michigan: Racine, WI; Chicago, IL; and East Chicago, IN. Individual water samples were collected one day a week for ten weeks between June and August. Samples were collected from eight specific sites made up of two river and six shoreline type environments. Sampling was completed at sites where various morphology (embayment, sand and sediment characteristics, size and shape) and hydrologic conditions (currents and waves) were present. Then samples were analyzed using microbial communities (metagenomic analysis), markers of contamination (microbial source tracking), and fecal indicator bacteria (E....
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Data were collected in August and September 2015 for analysis of bacteria communities of the Grand Calumet River and associated shorelines. Water samples were collected on three occasions corresponding to one rain-related (wet) events and two non-rain (dry) events. Water samples were collected in the Grand Calumet River, at the mouth of the river, at offshore locations around the peninsular impoundment and at shoreline locations: Jeorse Park (East Chicago, Indiana), Whihala (Whiting, Indiana), and 63rd Street (Chicago, Illinois) beaches. Samples were collected in triplicate, and water was filtered at the USGS Lake Michigan Ecological Research Station. After DNA extraction, samples were analyzed using 16S rRNA sequencing...


    map background search result map search result map USGS Soil Biogeochemistry 16S rRNA gene sequencing and E. coli for shorelines and the Grand Calumet River, Indiana, 2015 Microbial communities and bacterial indicators for shoreline sand, sediment, and water in Racine, Wisconsin; Chicago, Illinois; and East Chicago, Indiana; 2016-2017 Microbial Communities Across a Successional Gradient at Indiana Dunes National Park, 2015-2017 Influence of filter pore size on microbial communities and microbial source tracking (MST) markers on water in Racine, Wisconsin; Chicago, Illinois; East Chicago, Indiana, 2015-2017 Oriental Bittersweet Microbial Communities Across a Post Glacial Successional Gradient at Indiana Dunes National Park, 2017 Plant, soil, and microbial characteristics of marsh collapse in Mississippi River Deltaic wetlands Eutrophication and plankton communities (Cyanobacteria and eukaryotic algae) in the Grand Calumet River Area of Concern, Indiana, 2021 Plant, soil, and microbial characteristics of marsh collapse in Mississippi River Deltaic wetlands Microbial Communities Across a Successional Gradient at Indiana Dunes National Park, 2015-2017 16S rRNA gene sequencing and E. coli for shorelines and the Grand Calumet River, Indiana, 2015 Microbial communities and bacterial indicators for shoreline sand, sediment, and water in Racine, Wisconsin; Chicago, Illinois; and East Chicago, Indiana; 2016-2017 Influence of filter pore size on microbial communities and microbial source tracking (MST) markers on water in Racine, Wisconsin; Chicago, Illinois; East Chicago, Indiana, 2015-2017