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The data being released were part of a project funded by the United States Environmental Protection Agency (USEPA). This study sought to identify the planktonic communities (cyanobacteria, eukaryotic algae) potentially contributing to eutrophication within the Grand Calumet River Area of Concern (AOC) in northern Indiana along the southern shore of Lake Michigan. In 2021, triplicate water samples were collected from five locations during three events, 4/19/21 and 4/20/21, 7/7/21, and 9/15/21. Water samples were processed and planktonic communities were determined by a DNA-based technology (algal metabarcoding). Sampling locations: 1. Columbia Avenue, Hammond, IN 2. Lake George drainage ditch, Hammond, IN 3. Indiana...
Categories: Data;
Tags: Aquatic Biology,
DNA sequencing,
East Chicago, Indiana,
Ecology,
Gary, Indiana,
Data were collected to determine the abundance of nitrogen-fixing microorganisms in Cladophora algae growing on rocks, breakwall structures, or submerged dreissenid mussel beds around southern Lake Michigan. Cladophora samples (N=33) were collected between June and September 2015 from three urban areas: (a) Jeorse Park, East Chicago, Indiana, (b) Calumet Beach, Chicago, Illinois, and (c) North Beach, Racine, Wisconsin, and a National Park site, Portage Lakefront, Indiana Dunes National Park, Indiana. Corresponding lake water samples (N=33) were collected approximately 15-20 feet away from submerged algal mats. Genomic DNA was extracted from water and processed algal samples. The abundance of nitrogen-fixing microorganisms...
The U.S. Geological Survey (USGS), in cooperation with the Great Lakes Restoration Initiative, collected data in 2017 to study the sources and occurrences of continual detections of high Escherichia coli (E. coli) detections at urban beaches along the Lake Michigan shoreline in northwest Indiana and northeastern Illinois. High E. coli detections cause the beaches to be closed for recreational use until additional samples verify that E. coli levels have fallen below the threshold of 235 counts per 100 ml. The project used microbial source tracking (MST) and metagenomics analyses to evaluate the sources of E. coli. This data release provides the phytoplankton, mictobial source tracking, and metagenomics components...
The data being released were part of a project funded by the Great Lakes Restoration Initiative (GLRI). This study sought to examine the influence of filter pore size (5.0 µm pre and 0.22 µm final filtration) on microbial communities and source-specific microbial source tracking (MST) markers at three locations along southern Lake Michigan: Racine, WI; Chicago, IL; and East Chicago, IN; between 2015 and 2017. In 2015, triplicate water samples were collected during three events, in 2016 individual water samples were collected during three events, and in 2017, individual water samples were collected one day a week for ten weeks between June and August. Samples were collected from twelve locations, two river, two river...
Categories: Data;
Tags: Aquatic Biology,
Chicago,
DNA sequencing,
East Chicago,
Environmental Health,
This dataset contains data tables of laboratory quality-control data associated with environmental samples analyzed for microbiological constituents at the Ohio Water Microbiology Laboratory of the U.S. Geological Survey (USGS) during federal fiscal years 2012-2017 (October 1, 2011 through September 30, 2017). The environmental samples were collected across the United States by the USGS National Water Quality Assessment Project and other projects in Water Science Centers. The microbiological constituents include total coliforms, Escherichia coli (E. coli), enterococci, coliphage (F-specific and somatic), aerobic endospores, and actinomycetes. These quality-control data can be used to assess the quality of microbiological...
Categories: Data;
Tags: USGS Science Data Catalog (SDC),
United States of America,
bacteria,
laboratory methods,
microbiology
The U.S. Geological Survey, in cooperation with the Tennessee Department of Transportation, conducted an investigation of acid-rock drainage from road cuts in Tennessee during 2014-2015. The Devonian Chattanooga Shale contains disseminated pyrite and is a primary producer of acid-rock drainage (ARD) in Tennessee. One objective of the overall investigation was to attenuate ARD by manipulating the indigenous microbial community through different treatment injections. The scope of the study included establishing flow-through microcosms constructed with shale from the Chattanooga Shale formation rich in pyrite collected from an ARD site in Middle Tennessee. The microcosms were subjected to various treatments and evaluations...
Categories: Data;
Types: Map Service,
OGC WFS Layer,
OGC WMS Layer,
OGC WMS Service;
Tags: Hickman County,
USGS Science Data Catalog (SDC),
Williamson County,
acid-rock drainage,
geochemical processes,
The data associated with the following data release were collected between 2016 and 2017 at three locations on Lake Michigan: Racine, WI; Chicago, IL; and East Chicago, IN. Individual water samples were collected one day a week for ten weeks between June and August. Samples were collected from eight specific sites made up of two river and six shoreline type environments. Sampling was completed at sites where various morphology (embayment, sand and sediment characteristics, size and shape) and hydrologic conditions (currents and waves) were present. Then samples were analyzed using microbial communities (metagenomic analysis), markers of contamination (microbial source tracking), and fecal indicator bacteria (E....
Data were collected as part of a study to identify sources of E. coli contamination at several beaches located in the Grand Calumet River Areas of Concern, located in northern Indiana on Lake Michigan. The study was funded by the Great Lakes Restoration Initiative. Water samples were collected at each site (Jeorse Park 1, Jeorse Park 2, Hammond East, Hammond West, Whihala West, Whihala East, Whihala west breakwater, Hammond Marina, Whihala offshore locations, and the Grand Calumet River) one day a week or three times a week between 2015 and 2018. While the 2015 data were included in analysis, these data were previously publicly released https://doi.org/10.5066/F7H70F3D. Samples (water, sand, sediment) were analyzed...
A large spill of wastewater from oil and gas operations was discovered adjacent to Blacktail Creek near Williston, North Dakota in January 2015. To determine the effects of this spill on streambed microbial communities over time, bed sediment samples were taken from Blacktail Creek upstream, adjacent to, and at several locations downstream from the spill site. Blacktail Creek is a tributary of the Little Muddy River, and additional samples were taken upstream and downstream from the confluence of Blacktail Creek and the Little Muddy River. Samples were collected in February 2015, June 2015, June 2016, and June 2017. DNA was extracted from these sediments, and sequencing of the 16S ribosomal RNA gene was performed...
Categories: Data;
Tags: Aquatic Biology,
Blacktail Creek,
DNA sequencing,
Ecology,
Little Muddy River,
This dataset includes soil properties and microbial data from lake margin soils throughout the Yukon Flats National Wildlife Refuge. Data include multiple lakes that are defined as either stable or drying. Lake margin vegetation communities include graminoid, shrub, and forest. Sols are separated into organic and mineral layers. Microbial data includes different phospholipid fatty acids and soil enzyme activity data for multiple enzymes involved in C,N, and P cycling. Data also includes information on respiration rate constants (k) and labile substrate pool (Ao) from soil incubations.
Data were collected in August and September 2015 for analysis of bacteria communities of the Grand Calumet River and associated shorelines. Water samples were collected on three occasions corresponding to one rain-related (wet) events and two non-rain (dry) events. Water samples were collected in the Grand Calumet River, at the mouth of the river, at offshore locations around the peninsular impoundment and at shoreline locations: Jeorse Park (East Chicago, Indiana), Whihala (Whiting, Indiana), and 63rd Street (Chicago, Illinois) beaches. Samples were collected in triplicate, and water was filtered at the USGS Lake Michigan Ecological Research Station. After DNA extraction, samples were analyzed using 16S rRNA sequencing...
Categories: Data,
Data Release - Revised;
Tags: Contamination and pollution,
DNA sequencing,
Ecology,
Freshwater ecosystems,
Grand Calumet River,
The unicellular diazotrophic cyanobacterium Cyanothece sp. ATCC 51142 (Cyanothece 51142) is able to grow aerobically under nitrogen-fixing conditions with alternating light-dark cycles or continuous illumination. This study investigated the effects of carbon and nitrogen sources on Cyanothece 51142 metabolism via (13)C-assisted metabolite analysis and biochemical measurements. Under continuous light (50 mumol photons m(-2) s(-1)) and nitrogen-fixing conditions, we found that glycerol addition promoted aerobic biomass growth (by twofold) and nitrogenase-dependent hydrogen production [up to 25 mumol H(2) (mg chlorophyll)( -1) h(-1)], but strongly reduced phototrophic CO(2) utilization. Under nitrogen-sufficient conditions,...
Data were collected as part of a study to identify sources of E. coli contamination at several beaches located in the Grand Calumet River Areas of Concern, located in northern Indiana on Lake Michigan, as well as in Illinois and Wisconsin on Lake Michigan. Water samples were collected at each site in Indiana three times a week for thirteen weeks and at each site in Illinois and Wisconsin. All samples were analyzed for E. coli bacteria (an indicator bacteria for fecal contamination) and species-specific molecular markers (microbial source tracking, MST), including human, gull, and dog. Presence of MST markers indicates a fecal source at that location associated with the target animal. Field conditions were recorded...
Categories: Data,
Data Release - Revised;
Tags: Aquatic Biology,
Area of Concern,
Ecology,
Environmental Health,
Genetics,
Bacteria were isolated from seeds of non-native Phragmites australis (haplotype M) then representatives were evaluated for their capacities to become intracellular in root cells, and their effects on: 1.) germination rates and seedling growth, 2.) susceptibility to damping-off disease, and 3.) mortality and growth of competitor plant seedlings (dandelion (Taraxacum officionale F. H. Wigg) and curly dock (Rumex crispus L.)). The experiments included the following assessments of Phragmites-associated bacteria (Pseudomonas spp, strains Sandy LB4 (Pseudomonas fluorescens) and West 9 (Pseudomonas sp.)): capacity for plant growth promotion (using Poa annua seeds), Poa annua and Phragmites australis seed germination and...
Categories: Data;
Types: Citation;
Tags: Bosea thiooxidans,
Ecosystem functions,
Fusarium oxysporum,
Microbacterium oxydans,
Microbiology,
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