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Eddy covariance measures net ecosystem exchange of CO2 (NEE) at a scale between chamber-based measurements of CO2 exchange processes and large-scale models of CO2 flux dynamics. As the intermediate, it represents a link between small and large-scale estimates of NEE. Accuracy is therefore critical. However, estimates of nighttime ecosystem respiration based on scaled-up measurements of soil and leaf CO2 exchange are most often larger than from eddy covariance. Identifying the source of the discrepancy is difficult due to large measurement uncertainties associated with high variability of fluxes in complex ecosystems. This study compared measurements in a simple system that allowed for minimal uncertainty. We compared...