Filters: Tags: PCR (X)11 results (9ms)
Matrix inhibition PCR and Microtox® 81.9% screening assay analytical results for samples collected for the Sediment-Bound Contaminant Resiliency and Response Strategy pilot study, northeastern United States, 2015
Coastal communities are uniquely vulnerable to sea-level rise (SLR) and severe storms such as hurricanes. These events enhance the dispersion and concentration of natural and anthropogenic chemicals and pathogenic microorganisms that could adversely affect the health and resilience of coastal communities and ecosystems in coming years. The U.S. Geological Survey has developed the Sediment-Bound Contaminant Resiliency and Response (SCoRR) strategy to define baseline and post-event sediment-bound environmental health (EH) stressors. These data document toxicity measured by reduction of the light emission of Aliivibrio (formerly Photobacterium) fischeri and the inhibition of polymerase chain reactions caused by environmental...
Sequence analysis and comparison of rDNA ISR regions of Microcystis species from red tide and normal regions.
Microbial characterization of nitrification in a shallow, nitrogen-contaminated aquifer, Cape Cod, Massachusetts and detection of a novel cluster associated with nitrifying Betaproteobacteria
Groundwater nitrification is a poorly characterized process affecting the speciation and transport of nitrogen. Cores from two sites in a plume of contamination were examined using culture-based and molecular techniques targeting nitrification processes. The first site, located beneath a sewage effluent infiltration bed, received treated effluent containing O2 (> 300 µM) and NH4+ (51–800 µM). The second site was 2.5 km down-gradient near the leading edge of the ammonium zone within the contaminant plume and featured vertical gradients of O2, NH4+, and NO3− (0–300, 0–500, and 100–200 µM with depth, respectively). Ammonia- and nitrite-oxidizers enumerated by the culture-based MPN method were low in abundance at both...
Conventional PCR results for detecting Tetracapsuloides bryosalmonae in fish tissue and environmental DNA water samples
We developed and validated conventional and quantitative real-time PCR assays for the detection of DNA from the myxozoan parasite Tetracapsuloides bryosalmonae, the causative agent of proliferative kidney disease in fish. Assays were tested on fish tissue and on field-collected water samples to assess diagnostic and environmental DNA capabilities. The specificity, sensitivity, and broad applicability of the present assays surpass previous methods for detecting T. bryosalmonae DNA from fish tissue and water samples.
Randomly amplified polymorphic DNA profile-based measures of genetic diversity in crayfish correlated with environmental impacts
Dinucleotide microsatellite loci in a migratory wood warbler (Parulidae: Limnothlypis swainsonii) and amplification among other songbirds
In situ detection of the Clostridium botulinum type C sub(1) toxin gene in wetland sediments with a nested PCR assay
Survival and activity of Pseudomonas sp. strain B13(FR1) in a marine microcosm determined by quantitative PCR and an rRNA-targeting probe and its effect on the indigenous bacterioplankton