Filters: Tags: Parasites (X) > partyWithName: U.S. Geological Survey (X)

Filters Date Range Past year (2) Past month (1) Past week (0) Past day (0) Custom range... To   Search Types Citation (1) Contacts Patrick R Hutchins Adam J Sepulveda U.S. Geological Survey - ScienceBase Ecosystems Thierry M Work More... Categories Data (10) Tag Types Theme (12) Place (12) USGS Scientific Topic Keyword (6) Harvest Set (9) Tag Schemes Common geographic areas (10) USGS Thesaurus (12) None (5) ISO 19115 Topic Category (10) NA (1) View Results as: JSON ATOM CSV

Relating tissue egg counts to eggs/g of spleen in Hawaiian green turtles The present study proposes a new methodology for the quantification of parasite eggs in animal tissue. Quantification of parasites are important to understand epidemiology of spirorchiid infections in sea turtles, however different methodologies for quantifying Spirorchiidae eggs in turtle tissues have been used. The most representative way to quantify Spirorchiidae burdens in tissues is counting eggs / g of tissue, however, this method is very laborious. As an alternative, we propose quantifying number of Spirorchiidae egg/ area of tissue on a microscope slide. We compared this method to number of eggs / slide, a common metric of egg burden in turtle tissues. Both methods correlated well with eggs / g with eggs/mm2... Categories: Data; Tags: Hawaii, USGS Science Data Catalog (SDC), Wildlife Disease, climatologyMeteorologyAtmosphere, parasites, All tags... pathology, spirorchiid, Fewer tags Conventional and quantitative PCR assays for detecting Tetracapsuloides bryosalmonae in fish tissue and environmental DNA water samples We developed and validated conventional and quantitative real-time PCR assays for the detection of DNA from the myxozoan parasite Tetracapsuloides bryosalmonae, the causative agent of proliferative kidney disease in fish. Assays were tested on fish tissue and on field-collected water samples to assess diagnostic and environmental DNA capabilities. The specificity, sensitivity, and broad applicability of the present assays surpass previous methods for detecting T. bryosalmonae DNA from fish tissue and water samples. Types: Citation; Tags: Aquatic Biology, California, Genetics, Idaho, Montana, All tags... Tetracapsuloides bryosalmonae, USGS Science Data Catalog (SDC), Wildlife Disease, Wyoming, environmental DNA, molecular biology, parasites, Fewer tags Simple sequence repeats (SSRs) of Tetracapsuloides bryosalmonae parasites in kidney tissue collected from various water bodies in Montana, USA from 2016 to 2019 This dataset contains simple sequence repeat genotypes from Tetracapsuloides bryosalmonae infecting the tissue of fish collected from various locations throughout western and central Montana, USA. Categories: Data; Tags: Montana, Upper Yellowstone, aquatic biology, biota, parasites Molecular detection of Tetracapsuloides bryosalmonae DNA from fish tissues and environmental DNA water samples collected throughout the Inter-mountain West, USA from 2011 to 2019 This dataset contains molecular diagnostic results of the myxozoan parasite Tetracapsuloides byrosalmonae in fish tissue collected from the inter-mountain West, USA. Categories: Data; Tags: California, Idaho, Montana, Upper Snake, Upper Yellowstone, All tags... aquatic biology, farming, parasites, Fewer tags Spatial and temporal survey of waterborne myxozoan parasites in the Lake Sammamish watershed, Washington, from 2019 - 2020 There is a fundamental knowledge gap on the distribution, prevalence, intensity, and ecology of salmonid myxozoan parasites in the Lake Sammamish watershed, Washington. To address this knowledge gap, we tested water samples for Ceratonova shasta, Parvicapsula minibicornis and Tetracapsuloides bryosalmonae DNA from 84 sites distributed throughout the Lake Sammamish watershed in fall 2019 and 74 sites in spring 2020. Our surveillance identified zones with high waterborne parasite loads and provides a proof of concept for this approach that could be expanded throughout the larger Lake Washington watershed. Categories: Data; Tags: Aquatic Biology, Ceratonova shasta, Ecology, Parvicapsula minibicornis, Puget Sound, All tags... Tetracapsuloides bryosalmonae, USGS Science Data Catalog (SDC), Washington, Wildlife Disease, biota, environment, fishes, inlandWaters, parasites, Fewer tags T. bryosalmonae detection in fish and water, DNA sequence, and simple sequence repeat data collected in the Inter-Mountain West from 2011 to 2019 This data release contains molecular diagnostic results and molecular detection of the myxozoan parasite Tetracapsuloides byrosalmonae in fish tissue and environmental DNA samples collected from the inter-mountain West, USA. Tags: California, Idaho, Montana, USGS Science Data Catalog (SDC), Upper Snake, All tags... Upper Yellowstone, aquatic biology, biota, ecology, farming, genetics, parasites, Fewer tags Cytochrome oxidase subunit I (COI) and internally transcribed spacer region I (ITS1) DNA sequences of Tetracapsuloides bryosalmonae parasites in kidney tissue collected from various water bodies in Montana, USA from 2016 to 2019 This dataset contains DNA sequences of two genomic regions, cytochrome oxidase subunit one and internally transcribed spacer region one, obtained from Tetracapsuloides bryosalmonae infecting the tissue of fish collected from various locations throughout western and central Montana, USA. Categories: Data; Tags: Montana, Upper Yellowstone, aquatic biology, biota, parasites DNA sequences from Ichthyophonid parasites Data included are DNA sequences used to identify species boundaries among Ichthyophonus-like parasites. Sequences were generated for three gene regions from each parasite isolate. DNA sequences can be downloaded from GenBank (https://www.ncbi.nlm.nih.gov/) as text or fasta files. Accession numbers for each sequence are included in the data table (.csv file). Data includes 87 sequences (99,383 total nucleotides). Once manuscript is accepted there are no restrictions on dissemination of these data. Categories: Data; Tags: Aquatic Biology, Bering Sea, DNA sequencing, Environmental Health, Genetics, All tags... North Atlantic, Northeast Pacific, Ocean, Puget Sound, USGS Science Data Catalog (SDC), USGS-EMA-LOW Fish and Wildlife Disease, Wildlife Disease, Yukon, farming, parasites, wildlife disease, Fewer tags LAMP assay for detection of Asian fish tapeworm Data are relative fluorescent units obtained from the FAM channel of a AmpliFire Isothermal Fluorometer (Agdia, Inc., Elkhart, USA) recorded every 10 sec during as isothermal (LAMP) amplification. The isothermal reaction is to detect the Asian fish tapeworm, Schyzocotyle acheilognathi [syn. Bothriocephalus]. Samples (column headers) are described in tabs and include 1) positive control dilution series, 2) DNA Extractions from AFT sensitivity samples, and 3) DNA Extracted from non-AFT specificity controls. Categories: Data; Tags: Asian fish tapeworm, LAMP assay, USGS National Wildlife Health Center, USGS Science Data Catalog (SDC), Wildlife Disease, All tags... eDNA, environmental DNA, invasive species, isothermal amplification, loop-mediated isothermal amplification, molecular detection, parasites, rapid assay, Fewer tags Environmental DNA robotic and manual sampling data, Yellowstone and Snake Rivers, 2017-2019 Environmental DNA detection results from samples collected using autonomous water sampling robots and manual approaches. Samples were collected in the Upper Yellowstone River (Montana) and Upper Snake River (Idaho/Wyoming) in 2018 and 2019. Samples were tested for the DNA of the following species: the waterborne protozoa Naegleria spp., the fish pathogen Tetracapsuloides bryosalmonae, Scomber japonicas (mackerel fish), kokanee salmon (Oncorhynchus nerka) and dreissenid mussel (Dreissena spp.). Categories: Data; Tags: Palisades Dam, USGS Science Data Catalog (SDC), Upper Snake, Upper Yellowstone, Yellowstone National Park North, All tags... biota, environmental DNA, invasive species, parasites, river systems, Fewer tags Parasite sample voucher numbers and 28S rRNA alignment file for parasites collected from California Giant Salamander (Dicamptodon ensatus) from Webb Creek, in the Bear Creek Redwoods Preserve in Santa Clara County, California, USA Multi sequence file (.msf) file used to construct a 28S rRNA phylogenetic tree of species of Euryhelmis and other heterophyid trematodes. Sequences were generated from metacercariae samples at U.S. Geological Survey National Wildlife Health Center (NWHC) and compared for base pair similarities with publicly available sequences from GenBank using BLAST (https://blast.ncbi.nlm.nih.gov/Blast.cgi). Selection of GenBank sequences to include in the .msf file was based on the number of base pair similarities (94% or higher identity to this study’s sequences), 98–100% coverage AND stage of parasite from which the sequence was generated. An excel file listing all parasites and DNA from NWHC vouchered with the University... Categories: Data; Tags: Bear Creek Redwoods Preserve, California Giant Salamander, Euryhelmis sp, Santa Clara, USGS Science Data Catalog (SDC), All tags... Webb Creek, Wildlife Disease, biota, infection, parasites, Fewer tags Caligus Clemensi prevalence and counts on Clupea pallasii from Port Angeles Harbor, WA and from a controlled laboratory experiment conducted at USGS Marrowstone Marine Field Station, WA We characterized a natural sea louse epizootic of Caligus clemensi and the effects of parasitism on Pacific herring Clupea pallasii in Port Angeles Harbor, WA, USA. Infestation prevalence on newly metamorphosed age0 Pacific herring reached 100% prevalence by mid-August. At this time, the mean louse intensity was 4.6 lice and a positive correlation occurred between louse intensity and Pacific herring body length. The epizootic then waned, with infestation prevalence decreasing to less than 25% and the mean parasite intensity falling below 1 louse by October. In controlled experiments, louse-induced mortality was negligible in controlled experiments. While skin injuries were not detected, motile lice preferentially... Categories: Data; Tags: Pacific Northwest, Port Angeles, USGS Science Data Catalog (SDC), biota, fish, All tags... health and disease, parasites, Fewer tags