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Filters: Tags: Pathology (X) > partyWithName: Thierry M Work (X)

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Fibropapillomatosis (FP) is a tumor disease of marine turtles associated with Chelonid herpesvirus 5 (ChHV5) that has historically been refractory to growth in tissue culture. Here, we show for the first time de novo formation of ChHV5-positive intranuclear inclusions in cultured green turtle cells, which is indicative for active lytic replication of the virus. The minimal requirements to achieve lytic replication in cultured cells included 1) either in-vitro culturing of ChHV5-positive tumor biopsies or organotypic cultures consisting of ChHV5-positive turtle fibroblasts in collagen rafts seeded with ChHV5-negative or -positive turtle keratinocytes and 2) keratinocyte maturation, which occurred naturally in explanted...
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The present study proposes a new methodology for the quantification of parasite eggs in animal tissue. Quantification of parasites are important to understand epidemiology of spirorchiid infections in sea turtles, however different methodologies for quantifying Spirorchiidae eggs in turtle tissues have been used. The most representative way to quantify Spirorchiidae burdens in tissues is counting eggs / g of tissue, however, this method is very laborious. As an alternative, we propose quantifying number of Spirorchiidae egg/ area of tissue on a microscope slide. We compared this method to number of eggs / slide, a common metric of egg burden in turtle tissues. Both methods correlated well with eggs / g with eggs/mm2...
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As grazers, sea urchins are keystone species in tropical marine ecosystems, and their loss can have important ecological ramifications. Die-offs of urchins are frequently described but their causesare often unclear, in part because systematic examinations of animal tissues at gross and microscopic level are not done. In some areas, urchins are being employed to control invasive marine algae. Here we describe the pathology of a mortality event in Tripneustes gratilla in Hawaii where urchins were being translocated to control invasive algae. Although we did not determine the cause of the mortality event, our investigation suggests that animals died from inflammation of the test and epidermal ulceration followed by...
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Only one virus, Avipox, has been documented in wild birds in Hawaii. Here, using immunohistochemistry and PCR, we found that two native threatened Hawaiian geese, one with multicentric histiocytoma and another with toxoplasmosis and one Laysan albatross with avian pox were infected with reticuloendotheliosis virus (REV). The virus was isolated from one of the geese by cell culture. PCR surveys of other Hawaiian geese with various pathologies, avian pox cases, and pox viral isolates failed to reveal REV suggesting the virus is uncommon, at least in samples examined. The full genome of the Gag, Pol, and Env genes were sequenced for all three infected birds and revealed geographic divergence of the Pol gene suggesting...
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In 2010, a mass mortality of pufferfish in Hawaii was dominated by Arothron hispidus showing aberrant neurological behaviors. Using pathology, toxinology, and field surveys, we implicated a series of novel, polar, marine toxins as a likely cause of this mass mortality. Our findings are striking in that 1) a marine toxin was associated with a kill of a fish species that is, itself, toxic; 2) we provide a plausible mechanism to explain clinical signs of affected fish, and 3) this epizootic likely depleted puffer populations. Whilst our data are compelling, we did not synthesize the toxin de-novo, we were unable to categorically prove that the polar toxins caused mortality or that they were metabolites of an undefined...
    map background search result map search result map Puffer Mortality Data Chelonid herpesvirus 5 virus isolation data Relating tissue egg counts to eggs/g of spleen in Hawaiian green turtles Data set on reticuloendotheliosis in Hawaiian birds Mass mortality of collector urchins (Tripneustes gratilla) in Hawai`i Relating tissue egg counts to eggs/g of spleen in Hawaiian green turtles Mass mortality of collector urchins (Tripneustes gratilla) in Hawai`i Chelonid herpesvirus 5 virus isolation data Puffer Mortality Data Data set on reticuloendotheliosis in Hawaiian birds