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We developed and tested a new method for in situ characterization and distribution of inorganic particles in biopsied lung tissue from three diverse human subject groups using field emission scanning electron microscopy with an energy dispersive analyzer. Backscattered electron and secondary electron images of particulate matter in lung tissue were acquired. Inorganic particulate matter in the field of view of the image was analyzed using energy dispersive spectroscopy and subsequently identified and cataloged.
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We developed and tested a new method for in situ characterization and distribution of inorganic particles in biopsied lung tissue from three diverse human subject groups using field emission scanning electron microscopy with an energy dispersive analyzer. A backscattered electron image of each frame was acquired to locate inorganic particulate matter. The electron beam was deflected to points on the particles in the field of view to acquire an energy dispersive spectrum. The acquired spectra were used to identify the particle type.
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We developed and tested a new method for in situ characterization and distribution of inorganic particles in biopsied lung tissue from three diverse human subject groups using field emission scanning electron microscopy with an energy dispersive analyzer. The ICP-MS data table provides the concentrations of elements (in parts per billion) analyzed in the filtrate solutions from the tissue digestion method. This step was done to help determine if the phases identified during the in situ examination might dissolve during tissue digestion in a bleach solution.
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We developed and tested a new method for in situ characterization and distribution of inorganic particles in biopsied lung tissue from three diverse human subject groups using field emission scanning electron microscopy with an energy dispersive analyzer. This library of phase spectra is provided to show the typical energy dispersive spectrum of the inorganic particulate matter identified in lung biopsy sections.
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We developed and tested a new method for in situ characterization and distribution of inorganic particles in biopsied lung tissue from three diverse human subject groups using field emission scanning electron microscopy with an energy dispersive analyzer. The following .csv files summarize the inorganic particle identifications made in each frame of each area for each subject in the study. The In_Situ_Frame_Summary_2016-09-09_NJH***.csv is a tabulation of the phases identified in each frame. The In Situ Area Summary_2016-09-09.csv totals the number of times each phase was identified in an area of the lung tissue biopsy for each subject from the In_Situ_Frame_Summary_2016-09-09_NJH***.csv. The In Situ Subject Summary_2016-09-09.csv...
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We developed and tested a new method for in situ characterization and distribution of inorganic particles in biopsied lung tissue from three diverse human subject groups using field emission scanning electron microscopy with an energy dispersive analyzer. Secondary and backscattered electron images were acquired to document the size and location of inorganic particulate matter in areas of lung tissue biopsy sections.