Filters: Tags: Ploidy (X)
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In 2015-2016, the Ohio Division of Wildlife’s undercover law enforcement purchased 1,200 grass carp (Ctenopharyngodon idella). Fish heads and eyeballs were sent overnight to U.S. Geological Survey Wetland and Aquatic Research Center for ploidy analysis. Field and laboratory standard operating procedures were established and followed. Fish lengths, fish weights, and eyeball weights were obtained from the U.S. Fish and Wildlife Service’s feral carp ploidy program for grass carp and black carp (Mylopharyngodon piceus) and the Ohio grass carp. Internal 2µm or 4µm bead standards were used in establishing nuclear sizes from Nile tilapia (Oreochromis niloticus), known diploid (n=20) and triploid (n=20) carp blood, as well...
Data consists of ploidy, otolith stable isotope analysis and microchemistry, age, weight, sex, length and geolocation data from wild caught black carp (Mylopharyngodon piceus) captured in the Mississippi River basin from 2011 through 2018.
Data consist of flow cytometry files (.FCS) that were generated in the analysis of larval pallid sturgeon specimens. Nuclei from produced larval sturgeon somatic cells were analyzed using flow cytometry to estimate genome size and assign ploidy. Freshly collected rainbow trout (Oncorhynchus mykiss), largemouth bass (Micropterus salmoides), or pallid sturgeon blood were used as external controls, whereby the fish blood samples were analyzed in separate tubes from the cells of prepared sturgeon larvae. All samples at 1 x 10^6 cells/mL were incubated in a propidium iodide solution to stain DNA, then analyzed by flow cytometry. Analysis software generated histograms from the experimental samples and the control standard...
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