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Eutrophication and plankton communities (Cyanobacteria and eukaryotic algae) in the Grand Calumet River Area of Concern, Indiana, 2021 The data being released were part of a project funded by the United States Environmental Protection Agency (USEPA). This study sought to identify the planktonic communities (cyanobacteria, eukaryotic algae) potentially contributing to eutrophication within the Grand Calumet River Area of Concern (AOC) in northern Indiana along the southern shore of Lake Michigan. In 2021, triplicate water samples were collected from five locations during three events, 4/19/21 and 4/20/21, 7/7/21, and 9/15/21. Water samples were processed and planktonic communities were determined by a DNA-based technology (algal metabarcoding). Sampling locations: 1. Columbia Avenue, Hammond, IN 2. Lake George drainage ditch, Hammond, IN 3. Indiana... Categories: Data; Tags: Aquatic Biology, DNA sequencing, East Chicago, Indiana, Ecology, Gary, Indiana, All tags... Genetics, Grand Calumet River, Hammond, Indiana, Lake Michigan, Microbial Communities, USGS Science Data Catalog (SDC), Water Quality, biota, ecology, freshwater ecosystems, microbiology, polymerase chain reaction, water sampling, Fewer tags Quantification of nitrogen-fixing microorganisms (targeting nifH gene) in Cladophora algae by quantitative polymerase chain reaction (qPCR) Data were collected to determine the abundance of nitrogen-fixing microorganisms in Cladophora algae growing on rocks, breakwall structures, or submerged dreissenid mussel beds around southern Lake Michigan. Cladophora samples (N=33) were collected between June and September 2015 from three urban areas: (a) Jeorse Park, East Chicago, Indiana, (b) Calumet Beach, Chicago, Illinois, and (c) North Beach, Racine, Wisconsin, and a National Park site, Portage Lakefront, Indiana Dunes National Park, Indiana. Corresponding lake water samples (N=33) were collected approximately 15-20 feet away from submerged algal mats. Genomic DNA was extracted from water and processed algal samples. The abundance of nitrogen-fixing microorganisms... Categories: Data; Tags: Algae and water samples, Great Lakes Region, Lake Michigan, Nearshore submerged vegetation, USGS Science Data Catalog (SDC), All tags... algae, biota, economy, freshwater ecosystems, microbiology, nuisance species, nutrient cycling, polymerase chain reaction, Fewer tags Environmental DNA detection and survival, influence of sediment, and stream transport in a Lake Michigan watershed, 2018 The data released are associated with an examination of eDNA from round goby fish (Neogobius melanostomus) in a series of field sample collections (lake nearshore and stream transport) and in vitro laboratory experiments. The round goby was used as a model for our source of eDNA in the field collections and mesocosm experiments. The field samples for lake nearshore (water and sediment) were collected from two Lake Michigan shoreline locations (Portage Lakefront, Portage, Indiana and Washington Park, Michigan City, Indiana) to examine the detectability of eDNA, the influence of sediment on eDNA estimates, and eDNA spatial and temporal resolution. The field samples for stream transport (water) were collected from... Categories: Data; Tags: Aquatic Biology, Brown Ditch, Ecology, Genetics, Indiana Dunes National Park, All tags... Lake Michigan, Portage Lakefront, USGS Science Data Catalog (SDC), Washington Park, biota, environment, environmental DNA, field methods, freshwater ecosystems, inlandWaters, polymerase chain reaction, Fewer tags Influence of filter pore size on microbial communities and microbial source tracking (MST) markers on water in Racine, Wisconsin; Chicago, Illinois; East Chicago, Indiana, 2015-2017 The data being released were part of a project funded by the Great Lakes Restoration Initiative (GLRI). This study sought to examine the influence of filter pore size (5.0 µm pre and 0.22 µm final filtration) on microbial communities and source-specific microbial source tracking (MST) markers at three locations along southern Lake Michigan: Racine, WI; Chicago, IL; and East Chicago, IN; between 2015 and 2017. In 2015, triplicate water samples were collected during three events, in 2016 individual water samples were collected during three events, and in 2017, individual water samples were collected one day a week for ten weeks between June and August. Samples were collected from twelve locations, two river, two river... Categories: Data; Tags: Aquatic Biology, Chicago, DNA sequencing, East Chicago, Environmental Health, All tags... Genetics, Grand Calumet River, Illinois, Indiana, Lake Michigan, Microbial Communities, Racine, Root River Canal, USGS Science Data Catalog (SDC), Water Quality, Wisconsin, biota, ecology, freshwater ecosystems, microbiology, polymerase chain reaction, water sampling, Fewer tags Microbial communities and bacterial indicators for shoreline sand, sediment, and water in Racine, Wisconsin; Chicago, Illinois; and East Chicago, Indiana; 2016-2017 The data associated with the following data release were collected between 2016 and 2017 at three locations on Lake Michigan: Racine, WI; Chicago, IL; and East Chicago, IN. Individual water samples were collected one day a week for ten weeks between June and August. Samples were collected from eight specific sites made up of two river and six shoreline type environments. Sampling was completed at sites where various morphology (embayment, sand and sediment characteristics, size and shape) and hydrologic conditions (currents and waves) were present. Then samples were analyzed using microbial communities (metagenomic analysis), markers of contamination (microbial source tracking), and fecal indicator bacteria (E.... Categories: Data; Tags: Aquatic Biology, DNA sequencing, Ecology, Genetics, Grand Calumet River, All tags... Lake Michigan, Limnology, Microbial Communities, Root River Canal, USGS Science Data Catalog (SDC), Water Quality, ecology, farming, freshwater ecosystems, microbiology, polymerase chain reaction, water sampling, Fewer tags Quantitative polymerase chain reaction (qPCR): An alternative, rapid water quality monitoring tool at a National Park on Lake Michigan. Data were collected to evaluate the efficacy of quantitative polymerase chain reaction (qPCR) as a rapid, alternative method for monitoring recreational water at select beaches and rivers of Sleeping Bear Dunes National Lakeshore (SLBE), in Empire, Michigan. Water samples were collected between August 4 and September 18, 2014 (N=297) from four locations (Esch Rd, Otter Creek, Platte Bay, and Platte River). The samples were analyzed for indicator bacteria, E. coli and enterococci, by both culture-based (membrane filtration, MF; traditional method) and non-culture based (qPCR; rapid method). Recreational water quality standards (RWQS) and beach action values (BAV) were used as indices to compare water quality standard... Categories: Data; Tags: Lake Michigan, Sleeping Bear Dunes National Lakeshore, USGS Science Data Catalog (SDC), bacteria, biota, All tags... freshwater ecosystems, polymerase chain reaction, water quality, water sampling, Fewer tags Identify sources of high E. coli concentrations, beaches of southern Lake Michigan, 2015 (version 2.0, July 2020) Data were collected as part of a study to identify sources of E. coli contamination at several beaches located in the Grand Calumet River Areas of Concern, located in northern Indiana on Lake Michigan, as well as in Illinois and Wisconsin on Lake Michigan. Water samples were collected at each site in Indiana three times a week for thirteen weeks and at each site in Illinois and Wisconsin. All samples were analyzed for E. coli bacteria (an indicator bacteria for fecal contamination) and species-specific molecular markers (microbial source tracking, MST), including human, gull, and dog. Presence of MST markers indicates a fecal source at that location associated with the target animal. Field conditions were recorded... Categories: Data, Data Release - Revised; Tags: Aquatic Biology, Area of Concern, Ecology, Environmental Health, Genetics, All tags... Grand Calumet River, Great Lakes, Lake Michigan, USGS Science Data Catalog (SDC), contamination and pollution, environment, environmental health (human), freshwater ecosystems, microbiology, polymerase chain reaction, water sampling, Fewer tags