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We report the first simultaneous measurements of ?15N and ?13C of DNA extracted from surface soils. The isotopic composition of DNA differed significantly among nine different soils. The ?13C and ?15N of DNA was correlated with ?13C and ?15N of soil, respectively, suggesting that the isotopic composition of DNA is strongly influenced by the isotopic composition of soil organic matter. However, in all samples DNA was enriched in 13C relative to soil, indicating microorganisms fractionated C during assimilation or preferentially used 13C enriched substrates. Enrichment of DNA in 15N relative to soil was not consistently observed, but there were significant differences between ?15N of DNA and ?15N of soil for three...
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Changes in nutrient inputs due to aboveground herbivory may influence the litter and soil microbial community responsible for processes such as decomposition. The mesophyll-feeding scale insect (Matsucoccus acalyptus) found near Sunset Crater National Monument in northern Arizona, USA significantly increases pi�on (Pinus edulis) needle litter nitrogen (N) and phosphorus (P) concentrations by 50%, as well as litter inputs to soil by 21%. Because increases in needle litter quality and quantity of this magnitude should affect the microbial communities responsible for decomposition, we tested the hypothesis that insect herbivory causes a shift in soil microbial and litter microarthropod function. Four major findings...
We report the first simultaneous measurements of ?15N and ?13C of DNA extracted from surface soils. The isotopic composition of DNA differed significantly among nine different soils. The ?13C and ?15N of DNA was correlated with ?13C and ?15N of soil, respectively, suggesting that the isotopic composition of DNA is strongly influenced by the isotopic composition of soil organic matter. However, in all samples DNA was enriched in 13C relative to soil, indicating microorganisms fractionated C during assimilation or preferentially used 13C enriched substrates. Enrichment of DNA in 15N relative to soil was not consistently observed, but there were significant differences between ?15N of DNA and ?15N of soil for three...


    map background search result map search result map Impacts of herbivorous insects on decomposer communities during the early stages of primary succession in a semi-arid woodland Impacts of herbivorous insects on decomposer communities during the early stages of primary succession in a semi-arid woodland