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We examined the effect of chronic soil warming on microbial biomass, functional capacity, and community structure in soil samples collected from the Soil Warming Study located at the Harvard Forest Long-term Ecological Research (LTER) site. Twelve years of chronic soil warming at 5 �C above the ambient temperature resulted in a significant reduction in microbial biomass and the utilization of a suite of C substrates which included amino acids, carbohydrates, and carboxylic acids. Heating significantly reduced the abundance of fungal biomarkers. There was also a shift in the mineral soil microbial community towards gram positive bacteria and actinomycetes. Published in Soil Biology and Biochemistry, volume 40, issue...