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Summary Non-point nitrogen (N) loss from agriculture is an environmental concern among scientists, decision-makers, and the public. This study investigated NO3–N and total N losses from a mixed land use watershed (39.5 ha) in the Appalachian Valley and Ridge Physiographic Province as influenced by hydrology (flow type, runoff volume, storm sizes, and precipitation amount) and seasons (pre-growing, growing, and post-growing seasons) from 2002 to 2006. Stream discharge was monitored every 5-min and water samples for NO3–N and total N analyses were collected weekly for base flow and for every storm. The majority of NO3–N (about 75%) and total N (about 65%) were exported in base flow, which contributed about 64% of...