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This work is part of a study of the immunological effects of exposure to alternative flame retardants in avian species. For the pathology portion of the study, hatchling American kestrels (Falco sparverius) were exposed to the flame retardant isopropyl triphenyl phosphate (ITP) and then challenged with a synthetic analogue of viral double-stranded RNA, polyinosinic:polycytidylic acid (poly I:C). Control birds were challenged with vehicle only or vehicle and poly I:C. At euthanasia, spleen, thymus, and bursa of Fabricius were collected and fixed in 10% neutral buffered formalin for histopathological assessment. Slides were processed and stained with hematoxalin and eosin as per standard procedure (Luna 1968). Quantitative...
This work is part of an experimental trial investigating the effects of microclimate conditions of temperature and humidity on a fungal pathogen, Pseudogymnoascus destructans (Pd), that causes white-nose syndrome (WNS) disease in hibernating bats. As part of the trial, tri-colored bats (Perimyotis subflavus) were exposed to Pseudogymnoascus destructans (Pd) and allowed to hibernate in chambers with a variety of temperature and humidity conditions. Bats were euthanized after 83 days. A portion of the wing was rolled around dental wax dowels, fixed in 10% neutral buffered formalin, processed and stained with periodic acid-Schiff, and assessed by light microscopy for evidence of fungal infection. Three types of cutaneous...
This dataset describes histopathological changes in liver, kidney, heart, skeletal muscle and intestine of captive American kestrels exposed to the second-generation anticoagulant rodenticide brodifacoum (BROD). The goal of the study was to determine the toxic range of brodifacoum by feeding birds a diet containing 0.3, 1.0, or 3.0 ug BROD/g wet weight. Birds were necropsied and examined grossly for hemorrhages or anemia, and liver, kidney, heart, pectoral muscle, and intestine was collected for histopathological evaluation. Tissues were scanned at least 100x magnification and all lesions, including hemorrhage, inflammation, and degenerative changes, were described and assigned a morphologic diagnosis with severity,...
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This work is part of a study investigating the movement of microcystin from aquatic to terrestrial ecosystems via trophic transfer. Little brown bats (Myotis lucifugus), feeding opportunistically on aquatic insects including Hexagenia mayflies, were collected from a maternity roost near Little Traverse Lake (Leelanau County, Michigan, USA). Bats and fecal samples were collected for dietary analysis, quantification of microcystin in livers and feces, and histopathological evaluation of the liver. Liver was collected in RNAlater and stored frozen. Livers from three bats with the highest microcystin levels by ELISA were thawed, washed with PBS, fixed in 10% neutral buffered formalin, processed routinely for histopathology,...
This work is part of a study of the immunological effects of exposure to alternative flame retardants in avian species. For the pathology portion of the study, spleens and bursas from American kestrels (Falco sparverius) exposed by egg injection to varying doses of short-chain chlorinated paraffins (SCCPs) and the flame retardant TBBPA-BDBPE were examined microscopically for architectural and cellular abnormalities. At euthanasia, spleen and bursa of Fabricius were collected and fixed in 10% neutral buffered formalin for histopathological assessment. Slides were processed and stained with hematoxalin and eosin as per standard procedure (Luna 1968). Quantitative and qualitative B and T cell parameters were assessed...
This work is part of a study demonstrating that the fungus Nannizziopsis guarroi is the cause of the disease known as yellow fungus disease in bearded dragons (Pogona vitticeps) by fulfilling Koch’s postulates. In this study, bearded dragons were exposed to N. guarroi to assess gross and microscopic lesion development and the ability to re-culture the fungus from infected sites. At euthanasia, skin and internal organs were collected and fixed in 10% neutral buffered formalin for histopathological assessment. Slides were processed and stained with periodic acid-Schiff as per standard procedure (Luna 1968). Skin from exposed and control animals was assessed via light microscopy for the following parameters: type,...
Data are relative fluorescent units obtained from the FAM channel of a AmpliFire Isothermal Fluorometer (Agdia, Inc., Elkhart, USA) recorded every 10 sec during as isothermal (LAMP) amplification. The isothermal reaction is to detect the Asian fish tapeworm, Schyzocotyle acheilognathi [syn. Bothriocephalus]. Samples (column headers) are described in tabs and include 1) positive control dilution series, 2) DNA Extractions from AFT sensitivity samples, and 3) DNA Extracted from non-AFT specificity controls.
White-nose syndrome (WNS) caused by the fungus, Pseudogymnoascus destructans (Pd) has killed millions of North American insect-eating bats. Currently, methods to prevent the disease are limited. We conducted two trials to assess potential WNS vaccine candidates in wild-caught Myotis lucifugus. In a pilot study, we immunized bats with one of four vaccine treatments or PBS as a control and challenged them with Pd upon transfer into hibernation chambers. Bats in one vaccine-treated group, that received raccoon poxviruses (RCN) expressing Pd calnexin (CAL) and serine protease (SP), developed WNS at a lower rate (1/10) than other treatments combined (14/23), although samples sizes were small. The results of a second...
    map background search result map search result map Histopathology of little brown bats (Myotis lucifugus) collected from a maternity roost in Leelanau County, Michigan, USA, in June 2014 Histopathology of little brown bats (Myotis lucifugus) collected from a maternity roost in Leelanau County, Michigan, USA, in June 2014