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Freshwater mussels (Unionidae) are among the most imperiled groups of organisms in the world. Unionids are plagued with a lack of basic information regarding species distributions, life history characteristics, and ecological and biological requirements. We assessed the influence of hydrologic factors on the occurrence of the Suwannee Moccasinshell, Medionidus walkeri, a federally threatened freshwater mussel species endemic to the Suwannee River basin in Georgia and Florida. We also assessed the influence of survey effort on detection of M. walkeri during field surveys. All current (2013-2016) mussel survey records in the Suwannee River Basin were compiled, and cumulative discharge contributed by upstream springs...