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Throughout a 20-year biosurveillance period, viral hemorrhagic septicemia virus was isolated in low titers from only 6 / 7,355 opportunistically sampled adult Pacific herring, reflecting the typical endemic phase of the disease when the virus persists covertly. However, more focused surveillance efforts identified the presence of disease hot spots occurring among juvenile life history stages from certain nearshore habitats. These outbreaks sometimes recurred annually in the same temporal and spatial patterns and were characterized by infection prevalence as high as 96%. Longitudinal sampling indicated that some epizootics were relatively transient, represented by positive samples on a single sampling date, and others...
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From 2010-2018 we investigated the occurrence of avian influenza virus in wild birds in Iceland. A total of 6635 swabs samples were collected from wild birds or fecal material directly associated with wild birds. We screened all samples by a real time - polymerase chain reaction (RT-PCR) test with 381 testing positive. Further testing of all RT-PCR positive samples and all negative samples collected in 2012 by virus isolation yielded 120 positives, with 92 of those testing positive by RT-PCR for avian influenza virus.
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This dataset is one table with results of tests to determine exposure of Chukchi Sea polar bears to a suite of pathogens. Results are serological tests for antibodies and tests of fecal samples for presence of parasites. Serologic assays were conducted for Toxoplasma gondii, Neospora caninum, Francisella tularensis, Coxiella burnetii, Leptospira spp., Brucella canis, Brucella abortus/suis, and canine distemper virus. Parasitology analyses were conducted for presence of Giardia cysts and Cryptospiridium oocysts in fecal samples. Polar bears were sampled on sea ice in the Chukchi Sea between mid-March and early May 1987-1994 and 2008-2017. The dataset includes relevant information about the bears that were captured...
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Lake Sinai Viruses (LSV) are common ribonucleic acid (RNA) viruses of honey bees (Apis mellifera) that frequently reach high abundance but are not linked to overt disease. LSVs are genetically heterogeneous and collectively widespread, but despite frequent detection in surveys, the ecological and geographic factors structuring their distribution in A. mellifera are not understood. Even less is known about their distribution in other species. Better understanding of LSV prevalence and ecology have been hampered by high sequence diversity within the LSV clade. We developed a new genetic assay that detects all currently known lineages. We also performed pilot metagenetic sequencing to quantify the diversity of LSV...
To investigate the role of migratory birds in the dissemination of West Nile virus (WNV), we measured the prevalence of infectious WNV and specific WNV neutralizing antibodies in birds, principally Passeriformes, during spring and fall migrations in the Atlantic and Mississippi flyways from 2001-2003. Blood samples were obtained from 13,403 birds, representing 133 species. Specific WNV neutralizing antibody was detected in 254 resident and migratory birds, representing 39 species, and was most commonly detected in northern cardinals ( Cardinalis cardinalis ) (9.8%, N = 762) and gray catbirds ( Dumetella carolinensis ) (3.2%, N = 3188). West Nile virus viremias were detected in 19 birds, including 8 gray catbirds,...
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We report a draft genome of a previously undescribed calicivirus from a single brown bullhead inhabiting Lake Memphremagog, Vermont/Quebec. The genome is 7413 nt in length and is most similar to the Atlantic salmon calicivirus (nucleotide identity; 64.7%).
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The files in this data release are the processed DNA consensus sequence alignment files for the chelonid herpesvirus 5 (ChHV-5) genome plus 66 open reading frames representing protien-coding genes. DNA was extracted from tumor biopsies from green sea turtles (Chelonias mydas) with fibropapillomatosis sampled from Florida and Hawaii coastal waters. We used high-throughput short-read sequencing (Illumina MiSeq and Ion Torrent Personal Genome Machine) of long-range PCR products amplified from tumor tissue using primers designed from the single available ChHV5 reference genome from a Hawaii green sea turtle. The raw sequence data is available at the NCBI Sequence Read Archive under bioproject number PRJNA360405, including...
Tags: Class Reptilia, DNA sequencing, Earth, Oceans, Atlantic, North Atlantic, Earth, Oceans, Pacific, Family Cheloniidae, All tags...
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The threat posed by infectious diseases are a major concern for global public health and the role of migratory birds in pathogen transmission is increasingly under scrutiny. Enteric viral pathogen surveillance in migratory birds is a critical component of outbreak preparedness. The shedding of such pathogens through excrement into bodies of water located at migratory stop-over sites and breeding and wintering grounds creates conditions to potentially infect millions of birds annually. The mallard is the most abundant of all duck species and migratory across much of its range. Here we characterized enteric viral pathogens from the cecum of 16 captive raised mallards (Anas platyrhynchos) from Wisconsin, USA using...
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The authors screened 1,397 feral horses (Equus caballus) on Sheldon National Wildlife Refuge, Nevada, United States, for IgM and IgG against flavivirus during 2004-2006, 2008, and 2009. Positive serum samples were tested for neutralizing antibodies to West Nile virus (WNV) and St. Louis encephalitis virus (SLEV). One animal was positive for antibody against WNV in 2004, but all others tested in 2004-2006 were negative. In 2008 and 2009, the authors found evidence of increasing seropositive horses with age, whereas seroprevalence of WNV decreased from 19% in 2008 to 7.2% in 2009. No horses were positive for antibody against SLEV. Being unvaccinated, feral horses can be useful for WNV surveillance.
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A survey of public honey bee sequence data was performed to detect infections by Lake Sinai Virus (LSV). The Sequence Read Archive of the National Center for Biotechnology Information (NCBI) was queried to identify accessions of RNA sequence data derived from honey bee. These were filtered as described below and then up to 50 million reads or read pairs were downloaded and searched against a reference database of conserved LSV sequence. Accessions with matches above a specified threshold were downloaded in their entirety and assembled into longer contiguous sequences (contigs). The result contigs were searched against each open reading frame (ORF) of the reference LSV genome present in the NCBI database (accession...


    map background search result map search result map Seroprevalence of West Nile virus in feral horses on Sheldon National Wildlife Refuge, Nevada, United States 2004-2006, 2008 and 2009 Prevalence of West Nile virus in migratory birds during spring and fall migration, 2001-2003 Targeted amplicon resequencing of chelonid alphaherpesvirus 5 from tumor samples collected from Chelonia mydas (green turtle) inhabiting Hawaiian and Florida coastal waters Genetic detection of Lake Sinai Virus in honey bees (Apis mellifera) and other insects Survey of viral hemorrhagic septicemia virus in Pacific herring throughout the North Pacific Ocean Dataset: Surveillance for Avian Influenza Virus in Iceland, 2010 – 2018 Data Supporting: Draft Genome Sequence of a Novel Calicivirus from a Brown Bullhead (Ameiurus nebulosus) from Lake Memphremagog, VT Pathogen Exposure Data for Chukchi Sea Polar Bears 1988-1994 and 2008-2017 Molecular Detection and Characterization of Highly Pathogenic H5N1 Avian Influenza Viruses in Wild Birds Inhabiting Western Alaska Provides Evidence for Three Independent Viral Introductions Survey of potential viral pathogens in the ceca of pen-raised mallards Data Supporting: Draft Genome Sequence of a Novel Calicivirus from a Brown Bullhead (Ameiurus nebulosus) from Lake Memphremagog, VT Molecular Detection and Characterization of Highly Pathogenic H5N1 Avian Influenza Viruses in Wild Birds Inhabiting Western Alaska Provides Evidence for Three Independent Viral Introductions Survey of potential viral pathogens in the ceca of pen-raised mallards Dataset: Surveillance for Avian Influenza Virus in Iceland, 2010 – 2018 Survey of viral hemorrhagic septicemia virus in Pacific herring throughout the North Pacific Ocean Genetic detection of Lake Sinai Virus in honey bees (Apis mellifera) and other insects Targeted amplicon resequencing of chelonid alphaherpesvirus 5 from tumor samples collected from Chelonia mydas (green turtle) inhabiting Hawaiian and Florida coastal waters Pathogen Exposure Data for Chukchi Sea Polar Bears 1988-1994 and 2008-2017 Prevalence of West Nile virus in migratory birds during spring and fall migration, 2001-2003