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The data was gathered to document the extent and severity of biomarkers of exposure to estrogenic chemicals in smallmouth and largemouth bass. Samples were collected from 2004 through 2010 at sites in five watersheds within the Chesapeake Bay watershed. A total of 281 largemouth bass were collected from the Potomac, Susquehanna, Patuxent and Rappahannock rivers and 1,384 smallmouth bass from the Potomac, Susquehanna and James. Fish were collected by boat electroshocking, euthanized in the field, weighed, measured, a blood samples collected and a necropsy completed that included documenting any visible abnormalities and collecting tissues for histopathology. Plasma obtained from blood samples was analyzed for vitellogenin,...