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Straightened channels and altered and drained adjacent riparian wetlands have adversely impacted streams and rivers throughout the US Midwest. This research investigated the biological connection and water quality of a 0.07 ha diversion wetland and adjacent stream at the Olentangy River Wetland Research Park in central Ohio. Before the flowthrough conditions were established, we demonstrated with mark and recapture techniques that the wetland already was a biorefuge for fish under extreme conditions; two species (Centrarchidae) captured in the stream before a total drawdown of the stream were found in the wetland a year later. In addition, water at the bottom remained at around 4 °C over the winter likely due to...