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This dataset consists of measurements of the digestive gland in the freshwater snail Lymnaea stagnalis orally exposed to uranium. The objective of the study was to assess the morphological effects of uranium on the snail digestive gland by light microscopy. Laboratory-cultured L stagnalis were exposed to either synthetic or natural solid phases of uranium and a subset (6 controls and 6 exposed) were euthanized and fixed in 10% neutral buffered formalin for histopathological assessment. Slides were processed and stained with hematoxalin and eosin as per standard procedure (Luna 1968). The size of each sample, the length and width of digestive gland tubules (5 random glands per sample) and height of digestive cells...
Flea abundance and body condition data for black-tailed prairie dogs on sites treated and not treated with “FipBit” fipronil pellets. We sampled prairie dogs at Conata Basin, Buffalo Gap National Grassland, and Badlands National Park, South Dakota, USA. We sampled fleas from live-trapped prairie dogs during June–October 2018, May–October 2019, and July-August 2020. Prairie dogs were sampled before and after FipBit treatments. We anesthetized each prairie dog with isoflurane and combed it thoroughly for 30 seconds to dislodge fleas, which fell into a plastic bin. Fleas were counted and allowed to recover from anesthesia and placed back on prairie dogs, to minimize any removal effect. We weighed prairie dogs with...
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Throughout a 20-year biosurveillance period, viral hemorrhagic septicemia virus was isolated in low titers from only 6 / 7,355 opportunistically sampled adult Pacific herring, reflecting the typical endemic phase of the disease when the virus persists covertly. However, more focused surveillance efforts identified the presence of disease hot spots occurring among juvenile life history stages from certain nearshore habitats. These outbreaks sometimes recurred annually in the same temporal and spatial patterns and were characterized by infection prevalence as high as 96%. Longitudinal sampling indicated that some epizootics were relatively transient, represented by positive samples on a single sampling date, and others...
This work is part of an experimental trial to develop and assess novel recombinant raccoonpox virus (RCN) rabies vaccines in the mouse model, for potential use in bats. Briefly, our research group previously developed a recombinant RCN vaccine candidate expressing a mosaic glycoprotein (MoG) gene that protected mice and big brown bats when challenged with rabies virus (RABV). We developed two new recombinant RCN candidates expressing MoG (RCN-tPA-MoG and RCN-SS-TD-MoG) with the aim of improving RCN-MoG. We assessed and compared in vitro expression, in vivo immunogenicity, and protective efficacy in vaccinated mice challenged intracerebrally with RABV. In this data set, we share results of immunofluorescence assay...
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Data on body condition and reproduction of Utah prairie dogs at 5 colonies on the Awapa Plateau, Utah, USA, June-August 2013-2016. Utah prairie dogs were live-trapped and sampled on 5 colonies. We recorded the age (juvenile/adult) and mass (nearest 5 grams) of each prairie dog and marked its ears and body with metal tags and passive integrated transponders, respectively, for permanent identification. We measured each prairie dog's right hind foot length (nearest millimeter). We indexed each adult prairie dog's body condition as the ratio between its mass and hind-foot length. Prairie dogs were allowed to recover from anesthesia and released at their trapping locations. We indexed prairie dog reproduction, by colony...
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These data contain observation and null polygons for waterfowl aerial surveys of Lake Michigan collected through the years 2009-2014. Polygons were created adjacent to either side of the flight lines (transects). The right and left offset of the polygons from the flight path, was determined using the average altitude of the plane along the transect and the observation angle through the plane’s window. Observed birds were counted and identified by species. This count data was attributed to the polygon closest to the point along the transect where the observation occurred, and on the side of the plane in which the observation took place. The point data represent counts where each point represents a single species....
Snake fungal disease (SFD; ophidiomycosis), caused by the pathogen Ophidiomyces ophidiicola (Oo), has been documented in wild snakes in North America and Eurasia, and is a potentially emerging disease in the eastern USA. However, a lack of historical disease data has made it challenging to determine whether Oo is a recent arrival to the USA or whether SFD emergence is due to other factors. Here, we examined the genomes of 82 Oo strains to determine the pathogen’s history in the eastern USA. This dataset contains metadata on the strains used in that study.
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We investigated the dynamics of canine distemper virus (CDV) in grizzly bears (Ursus arctos) and wolves (Canis lupus) of the Greater Yellowstone Ecosystem (GYE) using serological data collected from 1984 to 2014. 565 sera samples were obtained from 425 unique grizzly bears (134 females and 291 males) from 1984 to 2014 and 319 sera samples were obtained from 285 unique wolves (130 females and 155 males) from 1996 to 2014. Here we provide the unique identifier for each individual, the species and sex, the date the individual was captured, the estimated birth year and age, and the CDV antibody titer results based upon serum neutralization assays.
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Raw sequencing data as generated by the five different methods used are provided for each of the three samples used in the comparison. The files are in FASTQ format as exported from the Oxford Nanopore’s MK1C using MinION flowcells. Files are labeled according to the method (as described in the paper) and the Sample ID). The MK1C exports data in blocks of 6000 reads per FASTQ file and all the FASTQ files from each method and sample are grouped in a common folder.
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Acanthurid surgeonfishes are an abundant and diverse group of herbivorous fishes on coral reefs. While their contribution to trophic linkages and dynamics in coral reef systems has received considerable attention, the role of linkages involving their parasites has not. As both consumers of fish tissue and prey to microcarnivores, external parasites can play a significant role in trophic transfer between the primarily algae-based food source and the broader coral reef community. Stable isotope analysis is a common tool for studying trophic linkages, which can be used for studies involving parasites. These data were collected to examine the stable isotope ecology (13C and 15N) of copepod (Caligus atromaculatus) and...
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The U.S. Geological Survey National Wildlife Health Center (NWHC) measured environmental contaminants in bald eagles (Haliaeetus leucocephalus) and golden eagles (Aquila chrysaetos) to evaluate dietary exposure to lead, mercury, and anticoagulant rodenticides (AR), which was identified by U.S. Fish and Wildlife Service (USFWS) as a priority issue of concern for the Mountain Prairie Region 6. Carcasses of bald eagles (n = 172) and golden eagles (n = 142) collected from North and South Dakota, Montana, Wyoming, Colorado, Utah, Nebraska, and Kansas between 2014-2017 were assessed for cause of death and liver lead, mercury, and AR levels. Trauma, electrocution, and lead poisoning were the 3 leading causes of death,...
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Dataset containing information for white-tailed deer samples from Ohio, Pennsylvania, Maryland, Virginia and New York, genotyped for 11 microsatellites markers. Marker OvirQ should not be used as it presents alleles inconsistent with reported pattern, with some alleles separated by only 1 base pair and inconsistent between runs. Projected coordinates representing sampling location are in a user-defined CRS, similar to USA Contiguous Albers Equal Area Conic: "+proj=aea +lat_1=29.3 +lat_2=45.3 +lat_0=23 +lon_0=-96 +x_0=0 +y_0=0 +ellps=GRS80 +towgs84=0,0,0,0,0,0,0 +units=m +no_defs".
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This dataset consists of one table with sample collection locations and results of screening resident wildlife for Cryptosporidium and Giardia in the Alaskan Arctic, 2012-2017. We analyzed feces collected from polar bear, Pacific walrus, Arctic fox, and caribou with MERIFLUOR direct immunofluorescence assay to determine the presence of Cryptosporidium oocysts and Giardia cysts.
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These data support a paired USGS publication and document the use of retention ponds on commercial poultry farms by wild waterfowl.
The data consist of levels of organic pollutants found in the blue mussels (Mytelus trossulus) samples from 44 sites along the Aleutian archipelago and in Cross Sound in southeast Alaska. For each site, the percent lipid, percent water and the sum of the target organic compounds are reported. The compounds are PCBs (polychlorinated biphenyls), DDTs (dichlorodiphenyltrichloroethanes), HCHs (hexachlorocyclohexanes), chlordane, and HCB (hexachlorocyclobenzene).
This work is part of a study of the immunological effects of exposure to alternative flame retardants in avian species. For the pathology portion of the study, hatchling American kestrels (Falco sparverius) were exposed to the flame retardant isopropyl triphenyl phosphate (ITP) and then challenged with a synthetic analogue of viral double-stranded RNA, polyinosinic:polycytidylic acid (poly I:C). Control birds were challenged with vehicle only or vehicle and poly I:C. At euthanasia, spleen, thymus, and bursa of Fabricius were collected and fixed in 10% neutral buffered formalin for histopathological assessment. Slides were processed and stained with hematoxalin and eosin as per standard procedure (Luna 1968). Quantitative...
The data consist of levels of organic pollutants found in the blood of sea otters captured from five areas in the western aleutian Islands and in Cross Sound in southeast Alaska. For each location, the sample size, and the median and range of values for each compound is provided. The compounds are PCBs (polychlorinated biphenyls), DDTs (dichlorodiphenyltrichloroethanes), HCHs (hexachlorocyclohexanes), chlordane, HCB (hexachlorocyclobenzene), and dieldrin.
These data represent capture mark recapture data from toads, and results of testing for Bd (Batrachochytrium dendrobatidis) the pathogen that causes amphibian chytridiomycosis on individuals. The data span from 2004-2016 at three sites in Montana where boreal toads were declining. The data also include temperature measurements at the sites and information on the egg counts and Bd prevalence from Columbia spotted frogs that are co-occurring at one of the sites.
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The present study proposes a new methodology for the quantification of parasite eggs in animal tissue. Quantification of parasites are important to understand epidemiology of spirorchiid infections in sea turtles, however different methodologies for quantifying Spirorchiidae eggs in turtle tissues have been used. The most representative way to quantify Spirorchiidae burdens in tissues is counting eggs / g of tissue, however, this method is very laborious. As an alternative, we propose quantifying number of Spirorchiidae egg/ area of tissue on a microscope slide. We compared this method to number of eggs / slide, a common metric of egg burden in turtle tissues. Both methods correlated well with eggs / g with eggs/mm2...


map background search result map search result map Canine distemper virus antibody titer results for grizzly bears and wolves in the Greater Yellowstone Ecosystem 1984-2014 Relating tissue egg counts to eggs/g of spleen in Hawaiian green turtles Stable isotope dynamics of herbivorous reef fishes and their ectoparasites: 2012, 2013, 2018 Flea abundance and body condition data for black-tailed prairie dogs on sites treated and not treated with “FipBit” fipronil pellets, South Dakota, 2018-2020 Data on Utah prairie dog body condition and reproductive success, Awapa Plateau, Utah, USA, 2013-2016 Levels of Persistent Organic Pollutants Found in Blue Mussels (Mytilus trossulus) in the Aleutian Archipelago and Southeast Alaska in 1994-1996 Levels of Persistent Organic Pollutants Found in the Blood of Sea Otters Captured in the Western Aleutian Islands and Southeast Alaska in 1997 Lake Michigan Sea Duck Survey 2009-2014 Survey of viral hemorrhagic septicemia virus in Pacific herring throughout the North Pacific Ocean USGS National Wildlife Health Center necropsy and contaminant results for bald and golden eagles collected in 8 States from January 1, 2014, through December 31, 2017 to determine cause of illness/death and lead, mercury, and anticoagulant rodenticide exposure Blacklegged tick nymph densities, tickborne pathogen prevalence, and white-tailed deer densities in eight national parks in the eastern United States from 2014-2022 Raw MinION FASTQ datafiles corresponding to the paper “A comparison of avian influenza virus whole genome sequencing approaches using nanopore technology” Data describing the use of retention ponds on commercial poultry facilities on Delmarva by wild waterfowl Cryptosporidium and Giardia Occurrence Data in Wildlife from Arctic Alaska, 2012-2017 Microsatellite genotypes and metadata for white-tailed deer samples from the Mid-Atlantic region of USA Raw MinION FASTQ datafiles corresponding to the paper “A comparison of avian influenza virus whole genome sequencing approaches using nanopore technology” Stable isotope dynamics of herbivorous reef fishes and their ectoparasites: 2012, 2013, 2018 Data describing the use of retention ponds on commercial poultry facilities on Delmarva by wild waterfowl Data on Utah prairie dog body condition and reproductive success, Awapa Plateau, Utah, USA, 2013-2016 Canine distemper virus antibody titer results for grizzly bears and wolves in the Greater Yellowstone Ecosystem 1984-2014 Lake Michigan Sea Duck Survey 2009-2014 Relating tissue egg counts to eggs/g of spleen in Hawaiian green turtles Blacklegged tick nymph densities, tickborne pathogen prevalence, and white-tailed deer densities in eight national parks in the eastern United States from 2014-2022 Microsatellite genotypes and metadata for white-tailed deer samples from the Mid-Atlantic region of USA Cryptosporidium and Giardia Occurrence Data in Wildlife from Arctic Alaska, 2012-2017 USGS National Wildlife Health Center necropsy and contaminant results for bald and golden eagles collected in 8 States from January 1, 2014, through December 31, 2017 to determine cause of illness/death and lead, mercury, and anticoagulant rodenticide exposure Survey of viral hemorrhagic septicemia virus in Pacific herring throughout the North Pacific Ocean Levels of Persistent Organic Pollutants Found in Blue Mussels (Mytilus trossulus) in the Aleutian Archipelago and Southeast Alaska in 1994-1996 Levels of Persistent Organic Pollutants Found in the Blood of Sea Otters Captured in the Western Aleutian Islands and Southeast Alaska in 1997