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Decomposition of grass leaf litter was studied on a shortgrass prairie using chemicals (HgCl2 and CuSO4) to prevent microbial activity (abiotic treatment), 53??m nylon mesh to exclude mesofauna (microbial treatment), and l?mm nylon mesh to allow the access of mesofauna. After 9 months, 15.2% of the blue grama grass litter was decomposed in the microbial treatment, and 29.4% was decomposed in the microbial plus mesofaunal treatment. After 7 months, 6.2% of the litter had disappeared from the abiotic treatment. There was a general decrease in C:N ratios with the microbial treatment lowest at the end of the experiment. Total available carbohydrates generally decreased with time. Certain mite families fluctuated with...