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The goal of this project was to quantify the background physiological condition of mussels in the Minnesota and St. Croix River basins using glycogen as an indicator of physiological stress. We obtained 230 samples of foot tissue from Lampsilis cardium (n=118) and Lasmigona complanata (n=112) from a total of four rivers during September 1-5, 2015. From each sample, we weighed out a 10.0 ± 2.0 mg aliquot for glycogen determination by the alkaline digestion and phenol-sulfuric acid spectrophotometric method. The accuracy of glycogen determinations was quantified by the use of procedural blanks, replicates of an in-house reference material, triplicate analysis of four aqueous calibration standards, duplicate analysis...
To quantify the responses of native mussel to the range of environmental conditions in the Minnesota River and St. Croix River basins, laboratory experiments will examine the physiological responses of mussels to different levels of suspended sediment and bed instability over a range of flow rates. Experiments will be conducted in the Outdoor StreamLab and flumes at the St. Anthony Falls Laboratory at the University of Minnesota. The experimental streams allow for direct manipulation of environmental conditions in a field-scale channel while collecting laboratory quality measurements. Laboratory experiments will focus on mussel response to suspended sediment (water quality) and bed instability (increased flows or...
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The American eel (Anguilla rostrata) is an imperiled fish hypothesized to use conspecific cues, in part, to coordinate long distance migration during their multi-stage life history. Here, tissues from multiple American eel life stages were collected and analyzed for the presence and concentration of bile acids. Specifically, samples were collected in the Northern Appalachian Research Laboratory during June 2016 from yellow eel liver, intestine, and gall bladder. Whole body bile acids were also evaluated in glass and elver stage American eel. All eels were held at Northern Appalachian Research Laboratory, Wellsboro, Pennsylvania, prior to sampling. Bile acid analysis was conducted using liquid chromatography coupled...
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We present TRAD: Thermal traits of anurans database for the Southeastern United States, a database of thermal trait values related to physiological (critical thermal minima and maxima, preferred temperature, mass) and behavioral thermoregulation (activity period, retreat emergence temperature, basking temperature, foraging temperature minimum and maximum) for 40 anuran species found within the southeastern United States. Using a species-centric approach, we collated this database by first identifying trait values from large reservoirs of amphibian ecology and natural history and then searching the literature using primarily Web of Science to thoroughly identify available thermal trait data. The TRAD database provides...
    map background search result map search result map Bile acid concentrations in tissues of American eel that were held at Northern Appalachian Research Laboratory, Wellsboro, Pennsylvania, as derived from liquid chromatography coupled to tandem mass spectrometry TRAD: Thermal traits of anurans database for the Southeastern United States Bile acid concentrations in tissues of American eel that were held at Northern Appalachian Research Laboratory, Wellsboro, Pennsylvania, as derived from liquid chromatography coupled to tandem mass spectrometry TRAD: Thermal traits of anurans database for the Southeastern United States