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Description of Work At two of Wisconsin's Areas of Concern (AOCs) on Lake Michigan, the Sheboygan River AOC and Milwaukee Estuary AOC, the USGS will assess whether sediment toxicity from PCBs, PAHs, selected metals, ammonia, or dissolved oxygen is present at acutely toxic or chronically toxic concentrations using sediment toxicity tests conducted with amphipods and midges. Study planning and literature search have been completed. Sediment was collected in October 2016 from sites in the two AOCs and sites in two non-AOC comparison sites. Assesments will be done at two additional sites: Kewaunee River and Oak Creek. Laboratory toxicity tests and chemical analyses are in progress, including sediment toxicity testing...