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This metadata record contains 15 tables with results from field and laboratory tests used to assess bioremediation in wetland areas at the Standard Chlorine of Delaware, Inc. Superfund site, New Castle County, Delaware, during 2019-2021. Included in the dataset are (1) polychlorinated biphenyl (PCB) concentrations in wetland sediment from sediment microcosm laboratory experiments, (2) chlorobenzene concentrations in wetland sediment from sediment microcosm laboratory experiments, (3) polychlorinated biphenyl concentrations from PCB microcosm laboratory experiments using polyethylene strips, (4) chlorobenzene concentrations from PCB microcosm laboratory experiments using polyethylene strips, (5) total iron and ferrous...
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The dataset supports a larger study that examined the impacts of three tackifiers (guar, psyllium, and polyacrylamide) on growth of two dryland mosses (Bryum argenteum and Syntrichia ruralis). Moss fragments were grown in petri dishes and subjected to individual tackifiers in one of three possible concentrations (0.5x, 1x, or 2x) of the respective manufacturer's recommended application rate. Distilled water was used as a control treatment, giving a total of ten treatments (nine tackifier-concentration combinations and a water control). Bryum fragments were watered four times daily for six weeks and Syntrichia fragments were watered twice daily for five weeks, after which the experiments were concluded. Shoot length,...
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This metadata record presents geochemical analytical data determined during laboratory experiments and a field pilot test collected during 2008-2010 to evaluate degradation of the cyclic nitramine explosive, hexahydro-1,3,4-trinitro-1,3,5-triazine (RDX), and perchlorate in a fractured rock aquifer. These experiments were conducted as part of a study to evaluate the feasibility of enhanced bioremediation for treatment of groundwater contaminants at White Sands Missile Range HTA site, New Mexico. Experiments included two anaerobic microcosm experiments, a culture enrichment test, and field injection test with the dechlorinating culture WBC-2. Data includes concentrations of nitrate, ammonium, perchlorate, RDX, and...
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Re-vegetation of mining wastes is difficult due to the inhospitable conditions for plant growth. Our aim was to determine whether the combined addition of municipal waste compost and plant growth promoting endophytes (i.e., microorganisms that live within plants) could improve plant growth, organic matter accumulation, and phytostabilization of metal contaminants across multiple types of hard rock mine waste. We grew a widespread perennial grass, Bouteloua curtipendula, for 45 days in tailings (Ag-Pb-Au mine) and waste rock (porphyry copper mine) sourced from southeastern Arizona, USA. We quantified organic matter accumulation, microbial biomass, plant growth rates, biomass yields, plant metal concentrations, and...
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The dataset supports a larger study that examined the impacts of three tackifiers (guar, psyllium, and polyacrylamide) on growth of two dryland mosses (Bryum argenteum and Syntrichia ruralis). Moss fragments were grown in petri dishes and subjected to individual tackifiers in one of three possible concentrations (0.5x, 1x, or 2x) of the respective manufacturer's recommended application rate. Distilled water was used as a control treatment, giving a total of ten treatments (nine tackifier-concentration combinations and a water control). Bryum fragments were watered four times daily for six weeks and Syntrichia fragments were watered twice daily for five weeks, after which the experiments were concluded. Shoot length,...
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Phytostabilization reduces the mobility of inorganic contaminants by establishing or enhancing plant growth. For small, remote, or abandoned mines, phytostabilization may reduce potential environmental hazards—provided plants can establish and grow. We grew a widespread perennial grass, Bouteloua curtipendula, in mining wastes with and without soil (compost, lime) and microbial amendments (endophyte seed coats) to determine whether we could improve plant establishment and growth. This data collection has four associated data releases: the physical elemental, and geochemical characteristics of the mining wastes and soil amendments; the laboratory environmental conditions during the growth of Bouteloua curtipendula...
Mining activities are a major source of land degradation in arid regions, and remediation methods developed for mesic sites may not be appropriate for arid sites. In climates where potential evapotranspiration exceeds precipitation, it might be possible to prevent the migration of contaminants away from a mine site by controlling the site water balance through vegetation, and allowing natural attenuation processes to reduce pollutant levels over time. We investigated the feasibility of remediating a nitrate-contaminated source-plume system in a desert environment using biological methods. The study site was a former uranium mill in Monument Valley, Arizona, where NO3? used in ore processing had leaked from the soil...
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A 45-day mesocosom experiment was conducted to determine how well a perennial grass species (Bouteloua curtipendula) grew in mining wastes from southeastern Arizona. The experiment tested whether the addition of an endophyte seed coat combined with a top dressing of compost improved grass growth, organic matter accumulation, and metal stabilization in the mining wastes (i.e., phytostabilization). The mining wastes were sourced from two formerly active hard rock mines: waste rock from a porphyry copper open pit mine and tailings from a tunnel and shaft polymetallic mine. The plants were grown for 45 days in an indoor growing complex. These data give the environmental conditions of the indoor growing complex (light,...
The biodegradation pathways of 1,1,2,2-tetrachloroethane (TeCA) and 1,1,2-trichloroethane (112TCA) and the associated microbial communities in anaerobic wetland sediments were evaluated using concurrent geochemical and genetic analyses over time in laboratory microcosm experiments. Experimental results were compared to in situ porewater data in the wetland to better understand the factors controlling daughter product distributions in a chlorinated solvent plume discharging to a freshwater tidal wetland at Aberdeen Proving Ground, Maryland. Microcosms constructed with wetland sediment from two sites showed little difference in the initial degradation steps of TeCA, which included simultaneous hydrogenolysis to 112TCA...
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The dataset supports a larger study that examined the impacts of three tackifiers (guar, psyllium, and polyacrylamide) on growth of two dryland mosses (Bryum argenteum and Syntrichia ruralis). Moss fragments were grown in petri dishes and subjected to individual tackifiers in one of three possible concentrations (0.5x, 1x, or 2x) of the respective manufacturer's recommended application rate. Distilled water was used as a control treatment, giving a total of ten treatments (nine tackifier-concentration combinations and a water control). Bryum fragments were watered four times daily for six weeks and Syntrichia fragments were watered twice daily for five weeks, after which the experiments were concluded. Shoot length,...
Globally, bioremediation is a common choice for remediation of petroleum hydrocarbon-contaminated sites. For application at cold climate sites, bioremediation approaches are appealing because they have potential to be more efficient and cost-effective than alternative, more energy intensive approaches. Several bioremediation approaches have been reported to be successful for petroleum hydrocarbon-contaminated soils at cold climate sites. In contrast, there are relatively few publications on applications of bioremediation for petroleum-contaminated groundwater at cold climate sites. Most of the existing relevant groundwater studies were conducted at sites with either no permafrost, or with sporadic to discontinuous...
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This dataset presents analytical results for laboratory and field tests conducted during 2015–19 as part of a bioremediation study in the wetland study area at Standard Chlorine of Delaware, Inc. Superfund site. Included in the dataset are (1) volatile organic compound and metal concentrations from field sampling in the wetland area, (2) results from in situ microcosms in the reactive barriers and control areas, and (3) volatile organic compound concentrations from laboratory experiments to test reactive mixtures for a barrier.
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This data release contains macro and microscale datasets describing the abundance, valence states, and mineral residence(s) of chromium, iron, and manganese in natural and synthetic mineral mixtures used in laboratory experiments designed to simulate dynamic conditions in an engineered aquifer system [see Izbicki and Groover (2018) and Miller and others (2020)]. The landing page contains primary metadata about this release and an overview "sample information" table containing descriptions of samples, their analyses, and their methods of preparation and preservation. As there is not a one-to-one correlation between samples and types of analyses performed, the overview table is an important navigation tool to locate...
Field biostimulation experiments at the U.S. Department of Energy's Integrated Field Research Challenge (IFRC) site in Rifle, Colorado, have demonstrated that uranium concentrations in groundwater can be decreased to levels below the U.S. Environmental Protection Agency's (EPA) drinking water standard (0.126 μM). During successive summer experiments – referred to as “Winchester” (2007) and “Big Rusty” (2008) - acetate was added to the aquifer to stimulate the activity of indigenous dissimilatory metal-reducing bacteria capable of reductively immobilizing uranium. The two experiments differed in the length of injection (31 vs. 110 days), the maximum concentration of acetate (5 vs. 30 mM), and the extent to which...
Populations of chemotactic bacteria are able to sense and respond to chemical gradients in their surroundings and direct their migration toward increasing concentrations of chemicals that they perceive to be beneficial to their survival. It has been suggested that this phenomenon may facilitate bioremediation processes by bringing bacteria into closer proximity to the chemical contaminants that they degrade. To determine the significance of chemotaxis in these processes it is necessary to quantify the magnitude of the response and compare it to other groundwater processes that affect the fate and transport of bacteria. We present a systematic approach toward quantifying the chemotactic response of bacteria in laboratory...
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These datasets contain microbial community data from groundwater samples collected at an in situ bioremediation site located at the former Naval Air Warfare Center (NAWC), West Trenton, NJ. DNA was extracted from groundwater samples collected from monitoring wells at the NAWC study site from July 2008 through July 2015 and analyzed for microbial community structure. Sample collection coincided with a groundwater bioremediation experiment investigating the microbial degradation of the contaminant trichloroethylene (TCE) prevalent in the targeted region of the aquifer. Nutrient addition and a microbial consortium, commercially developed to stimulate the degradation of TCE and TCE byproducts, was introduced to the...
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These data were compiled to support and inform the Bureau of Land Management’s Colorado Plateau Native Plant Program and to guide future management action when selecting regions to collect and increase seed for native plant materials development. The objective of our study was to develop geospatial datasets to aid land managers and restoration practitioners in identifying areas that will need to be restored in the future (currently disturbed) as well as areas to source new native plant materials for propagation with increased climate similarity to these areas across the Colorado Plateau, Arizona/New Mexico Mountains, and Arizona/New Mexico Plateaus. These data represent species distribution models for 12 high priority...
Tags: Achnatherum hymenoides, Arizona, Arizona/New Mexico Mountains, Arizona/New Mexico Plateaus, Astragalus lonchocarpus, All tags...
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Cr(VI) contaminated groundwater at Hinkley is undergoing bioremediation using added ethanol as a reductant in a volume of the aquifer defined as the In-situ Reduction Zone (IRZ). This treatment effectively reduces Cr(VI) to Cr(III) which is rapidly sequestered by sorption to aquifer particle surfaces and by co-precipitation within iron or manganese bearing minerals forming in place as reduction proceeds. Successful mitigation of the extant Cr(VI) plume is projected to require 90 to 220 years, at which time ethanol loading will likely cease. This projection assumes that Cr(VI) removal is permanent and that no Cr(III) will oxidize back to Cr(VI) in the event of changing hydrological conditions resulting in oxygen...
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Mining wastes can pose environmental hazards. These hazards can be mitigated by promoting the growth of native plants that can stabilize the mine wastes and potentially toxic elements in situ (i.e., phytostabilization). We grew a widespread perennial grass species (Bouteloua curtipendula) in dolomite amended polymetallic tailings from a historic mine in southeastern Arizona (USA). We applied a compost top dressing and an endophyte seed coating alone and in combination to quantify improvements in plant growth and potentially toxic trace element stabilization. This release provides the data on the following: temperature, light, and humidity during growth; plant growth rates and biomass yields; organic matter concentrations...
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The dataset supports a larger study that examined the impacts of three tackifiers (guar, psyllium, and polyacrylamide) on growth of two dryland mosses (Bryum argenteum and Syntrichia ruralis). Moss fragments were grown in petri dishes and subjected to individual tackifiers in one of three possible concentrations (0.5x, 1x, or 2x) of the respective manufacturer's recommended application rate. Distilled water was used as a control treatment, giving a total of ten treatments (nine tackifier-concentration combinations and a water control). Bryum fragments were watered four times daily for six weeks and Syntrichia fragments were watered twice daily for five weeks, after which the experiments were concluded. Shoot length,...


    map background search result map search result map Tackifier impacts on growth of Great Basin mosses Bryum argenteum and Syntrichia ruralis, a growth chamber study, 2017-2018 Tackifier impacts on growth of Great Basin moss Bryum argenteum, 2017-2018 Tackifier impacts on growth of Great Basin moss Syntrichia ruralis, 2017-2018 Mass adjustment ratios used for Great Basin mosses Bryum argenteum and Syntrichia ruralis, 2017-2018 Aqueous and Solid Phase Chemistry of Sequestration and Re-oxidation of Chromium in Experimental Microcosms with Sand and Sediment from Hinkley, CA Volatile organic compound and metal concentrations associated with field sampling and laboratory experiments and in situ microcosm results, wetland bioremediation study, Standard Chlorine of Delaware, Inc. Superfund site, New Castle County, Delaware, 2015–19 Optical Petrography, Bulk Chemistry, Micro-scale Mineralogy/Chemistry, and Bulk/Micron-Scale Solid-Phase Speciation of Natural and Synthetic Solid Phases Used in Chromium Sequestration and Re-oxidation Experiments with Sand and Sediment from Hinkley, CA Geochemical data from laboratory and field experiments conducted to evaluate degradation of perchlorate and RDX in groundwater, White Sands Missile Range HTA site, New Mexico Grass Growth in Mining Wastes with Compost and Endophyte Additions Environmental Conditions during Growth of Bouteloua curtipendula in Mining Wastes Quantification of Bouteloua curtipendula biomass, organic matter accumulation, and geochemical changes during growth in mining wastes Phytostabilization in Polymetallic Tailings using Compost and Endophyte Additions Microbial community analyses of groundwater collected during an enhanced bioremediation experiment of trichlorethylene in a fractured rock aquifer, West Trenton, NJ (2008-2015) Disturbance, energy, climate partitions, cultivars and species habitat data for the Colorado Plateau and environs Volatile organic compound, polychlorinated biphenyl, and biogeochemical data from passive samplers and laboratory experiments for wetland bioremediation study, Standard Chlorine of Delaware, Inc. Superfund Site, New Castle County, Delaware, 2019–2021 Microbial community analyses of groundwater collected during an enhanced bioremediation experiment of trichlorethylene in a fractured rock aquifer, West Trenton, NJ (2008-2015) Phytostabilization in Polymetallic Tailings using Compost and Endophyte Additions Volatile organic compound, polychlorinated biphenyl, and biogeochemical data from passive samplers and laboratory experiments for wetland bioremediation study, Standard Chlorine of Delaware, Inc. Superfund Site, New Castle County, Delaware, 2019–2021 Tackifier impacts on growth of Great Basin mosses Bryum argenteum and Syntrichia ruralis, a growth chamber study, 2017-2018 Tackifier impacts on growth of Great Basin moss Bryum argenteum, 2017-2018 Tackifier impacts on growth of Great Basin moss Syntrichia ruralis, 2017-2018 Mass adjustment ratios used for Great Basin mosses Bryum argenteum and Syntrichia ruralis, 2017-2018 Volatile organic compound and metal concentrations associated with field sampling and laboratory experiments and in situ microcosm results, wetland bioremediation study, Standard Chlorine of Delaware, Inc. Superfund site, New Castle County, Delaware, 2015–19 Aqueous and Solid Phase Chemistry of Sequestration and Re-oxidation of Chromium in Experimental Microcosms with Sand and Sediment from Hinkley, CA Optical Petrography, Bulk Chemistry, Micro-scale Mineralogy/Chemistry, and Bulk/Micron-Scale Solid-Phase Speciation of Natural and Synthetic Solid Phases Used in Chromium Sequestration and Re-oxidation Experiments with Sand and Sediment from Hinkley, CA Geochemical data from laboratory and field experiments conducted to evaluate degradation of perchlorate and RDX in groundwater, White Sands Missile Range HTA site, New Mexico Disturbance, energy, climate partitions, cultivars and species habitat data for the Colorado Plateau and environs