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Chelonid herpesvirus 5 virus isolation data Fibropapillomatosis (FP) is a tumor disease of marine turtles associated with Chelonid herpesvirus 5 (ChHV5) that has historically been refractory to growth in tissue culture. Here, we show for the first time de novo formation of ChHV5-positive intranuclear inclusions in cultured green turtle cells, which is indicative for active lytic replication of the virus. The minimal requirements to achieve lytic replication in cultured cells included 1) either in-vitro culturing of ChHV5-positive tumor biopsies or organotypic cultures consisting of ChHV5-positive turtle fibroblasts in collagen rafts seeded with ChHV5-negative or -positive turtle keratinocytes and 2) keratinocyte maturation, which occurred naturally in explanted... Categories: Data; Types: Map Service, OGC WFS Layer, OGC WMS Layer, OGC WMS Service; Tags: Hawaii, Herpesvirus, USGS Science Data Catalog (SDC), electron microscopy, immunohistochemistry, All tags... morphogenesis, pathology, pathology, Fewer tags Data on blood cells of the collector urchin, Tripneustes gratilla Echinoderms such as urchins are important in marine ecosystems, particularly as grazers, and unhealthy urchins can have important ecological implications. For instance, unexplained mortalities of Diadema sp. in the Caribbean were followed by algal overgrowth and subsequent collapse of coral reef ecosystems. Unfortunately few tools exist to evaluate echinoderm health making management of mortalities or other health issues problematic. Hematology is often used to assess animal health in many animal groups including invertebrates but is seldom applied to echninoderms. We used a standard gravitometric technique to concentrate fixed ceolomocytes from the collector urchin Tripneustes gratilla onto microscope slides permitting... Categories: Data; Tags: Oahu, Tripneustes gratilla, USGS Science Data Catalog (SDC), Wildlife Disease, biota, All tags... coelomocytes, echinoderms, electron microscopy, hematology, lectins, light microscopy, Fewer tags Sea star wasting disease pathology Sea star wasting disease (SSWD) is a suite of poorly described non-specific clinical signs including abnormal posture, epidermal ulceration, and limb autotomy (sloughing) causing mortalities of over 20 species of sea stars and subsequent ecological shifts throughout the northeastern Pacific. While SSWD is widely assumed to be infectious with environmental conditions facilitating disease progression, little data exist on cellular changes associated with disease that could inform mechanisms of disease pathogenesis and host susceptibility. Here, we replicated SSWD by exposing captive Pisaster ochraceus to a suite of non-infectious organic substances and show that development of gross lesions is a basal to surface process... Categories: Data; Tags: Bodega Bay, USGS Science Data Catalog (SDC), Wildlife Disease, biota, clinical pathology, All tags... echinoderms, electron microscopy, histopathology, light microscopy, Fewer tags