Filters: Tags: environmental DNA (X) > Types: Citation (X)
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This dataset includes quantifications of bigheaded carp DNA found in water samples collected from the Wabash River along transects at 3 sites over time. The samples were collected at 18 equidistant points in a transect across the river at each site. Samples were collected in 2013 on May 29, 30, 31, June 5, 6, 7, 8, 9, 10, 11, 16, 17, 19, 20, 21, and 22. Quantitative polymerase chain reaction (qPCR) was performed to determine the DNA concentrations in two replicates using the bigheaded carp assay defined in Merkes and others 2014.
Categories: Data;
Types: Citation,
Map Service,
OGC WFS Layer,
OGC WMS Layer,
OGC WMS Service;
Tags: Americus,
Asian carp,
Bigheaded carps,
Environmental DNA,
French Post,
We developed and validated conventional and quantitative real-time PCR assays for the detection of DNA from the myxozoan parasite Tetracapsuloides bryosalmonae, the causative agent of proliferative kidney disease in fish. Assays were tested on fish tissue and on field-collected water samples to assess diagnostic and environmental DNA capabilities. The specificity, sensitivity, and broad applicability of the present assays surpass previous methods for detecting T. bryosalmonae DNA from fish tissue and water samples.
Eggs were collected in the Upper Mississippi main stem (Pool 9 and Pool 11) during the summer of 2013. Using previously published morphological characteristics, eggs were positively identified as belonging to an invasive Asian carp genus. A subsample of these eggs was subsequently analyzed using molecular methods to determine species identity. Genetic identification of a total of 41 eggs was attempted using the cytochrome c oxidase 1 (COI) gene. Due to the preservation technique used (formalin) and resulting DNA degradation, sequences from only 17 individuals could be recovered. In all cases, non-carp cyprinids were identified as the most likely species identity (usually a Notropis spp.). In previously published...
Categories: Data;
Types: Citation,
Map Service,
OGC WFS Layer,
OGC WMS Layer,
OGC WMS Service;
Tags: Mississippi River,
USGS Science Data Catalog (SDC),
eDNA,
environmental DNA
We developed and validated conventional and quantitative real-time PCR assays for the detection of DNA from the myxozoan parasite Tetracapsuloides bryosalmonae, the causative agent of proliferative kidney disease in fish. Assays were tested on fish tissue and on field-collected water samples to assess diagnostic and environmental DNA capabilities. The specificity, sensitivity, and broad applicability of the present assays surpass previous methods for detecting T. bryosalmonae DNA from fish tissue and water samples.
Categories: Data;
Types: Citation;
Tags: California,
Idaho,
Montana,
PCR,
Proliferative Kidney Disease,
This dataset includes quantifications of bigheaded carp DNA found in water samples collected from the Wabash River along transects at 3 sites over time. The samples were collected at 18 equidistant points in a transect across the river at each site. Samples were collected in 2013 on May 29, 30, 31, June 5, 6, 7, 8, 9, 10, 11, 16, 17, 19, 20, 21, and 22. Quantitative polymerase chain reaction (qPCR) was performed to determine the DNA concentrations in two replicates using the bigheaded carp assay defined in Merkes and others 2014.
Categories: Data;
Types: Citation,
Map Service,
OGC WFS Layer,
OGC WMS Layer,
OGC WMS Service;
Tags: Americus,
Asian carp,
Bigheaded carps,
Environmental DNA,
French Post,
Community composition data from multi-pass electrofishing surveys for assessing fish populations in headwater streams of the Adirondack Mountains, New York, USA. Each row represents one individual fish. This spreadsheet contains 11 columns. The first 7 columns describe the data (year collected, local site ID, stream name, latitude, longitude, USGS site number, and electrofishing pass number) and the remaining 4 columns describe each individual fish (common name, scientific name, total length (mm) and weight (g)). These data support the following publication: Baldigo, B.P., Sporn, L.A., George, S.D., and Ball, Jacob, 2017. Efficacy of environmental DNA to detect and quantify Brook Trout Salvelinus fontinalis, populations...
Categories: Data;
Types: ArcGIS REST Map Service,
ArcGIS Service Definition,
Citation,
Downloadable,
Map Service;
Tags: Adirondack State Park,
Adirondack streams,
Adirondacks,
Aquatic Biology,
DNA,
A probe-based quantitative real-time PCR assay was developed to detect Tetracapsuloides bryosalmonae, which causes Proliferative Kidney Disease in salmonid fish, in kidney tissue and environmental DNA (eDNA) water samples. We present data that was used to determine assay sensitivity, specificity and to define the limits of detection and quantification.
Categories: Data;
Types: Citation;
Tags: East Gallatin River,
Montana,
Sypes Canyon Creek,
Tetracapsuloides bryosalmonae,
Yellowstone River,
Environmental DNA (eDNA) detection is a rapidly expanding technique used to non-invasively detect cryptic, low density, or logistically difficult-to-study species, such as imperiled manatees. Genetic material shed into the environment through tissue and body fluids is concentrated from water samples and analyzed for the presence of targeted eDNA. To help delineate manatee habitat ranges, high use areas, and seasonal population changes, a cytochrome-b quantitative PCR and state-of-the-art droplet digital PCR (ddPCR) eDNA assay was developed for the three extant and vulnerable manatee species: both subspecies of the West Indian manatee (Florida and Antillean), the African manatee and Amazonian manatee. Occurrence...
The data set is six separate csv files. Four of which contain the quantity of DNA copy numbers and fluor used to analyze the DNA quantities collected from water samples from four separate portions of the study (adult SL field, adult SL lab, larval SL field, larval SL lab) and need to be in their own csv file. Also included is a csv with adult SL trapping data, a csv for larval SL shocking data, and a csv with the volume that was filtered for our DNA extractions.
Categories: Data;
Types: Citation;
Tags: Black River, MI,
Canada,
Great Lakes,
Great Lakes,
Hammond Bay Biological Station,
This dataset includes quantifications of bigheaded carp DNA found in water samples collected from the Wabash River along transects at 3 sites over time. The samples were collected at 18 equidistant points in a transect across the river at each site. Samples were collected in 2013 on May 29, 30, 31, June 5, 6, 7, 8, 9, 10, 11, 16, 17, 19, 20, 21, and 22. Quantitative polymerase chain reaction (qPCR) was performed to determine the DNA concentrations in two replicates using the bigheaded carp assay defined in Merkes and others 2014.
Categories: Data;
Types: Citation,
Map Service,
OGC WFS Layer,
OGC WMS Layer,
OGC WMS Service;
Tags: Americus,
Asian carp,
Bigheaded carps,
Environmental DNA,
French Post,
This dataset includes quantifications of bigheaded carp DNA found in water samples collected from the Wabash River along transects at 3 sites over time. The samples were collected at 18 equidistant points in a transect across the river at each site. Samples were collected in 2013 on May 29, 30, 31, June 5, 6, 7, 8, 9, 10, 11, 16, 17, 19, 20, 21, and 22. Quantitative polymerase chain reaction (qPCR) was performed to determine the DNA concentrations in two replicates using the bigheaded carp assay defined in Merkes and others 2014.
Categories: Data;
Types: Citation,
Map Service,
OGC WFS Layer,
OGC WMS Layer,
OGC WMS Service;
Tags: Americus,
Asian carp,
Bigheaded carps,
Environmental DNA,
French Post,
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