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This dataset includes quantifications of bigheaded carp DNA found in water samples collected from the Wabash River along transects at 3 sites over time. The samples were collected at 18 equidistant points in a transect across the river at each site. Samples were collected in 2013 on May 29, 30, 31, June 5, 6, 7, 8, 9, 10, 11, 16, 17, 19, 20, 21, and 22. Quantitative polymerase chain reaction (qPCR) was performed to determine the DNA concentrations in two replicates using the bigheaded carp assay defined in Merkes and others 2014.
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The dataset is composed of four tables containing environmental DNA (eDNA) data, fish capture data, and site location information from Round Goby (Neogobius melanostomus) surveys conducted on parts of the Champlain Canal and upper Hudson River, and adjacent areas, in New York during 2022 and 2023. First posted May 5, 2022, ver. 1.0 Revised June 2022, ver. 2.0 Revised August 2022, ver. 3.0 Revised November 2022, ver. 4.0 Revised February 2023, ver. 5.0 Revised May 2023, ver. 6.0 Revised June 2023, ver. 7.0 Revised September 2023, ver. 8.0 Revised October 2023, ver. 9.0 Revised December 2023, ver. 10.0
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Eggs were collected in the Upper Mississippi main stem (Pool 9 and Pool 11) during the summer of 2013. Using previously published morphological characteristics, eggs were positively identified as belonging to an invasive Asian carp genus. A subsample of these eggs was subsequently analyzed using molecular methods to determine species identity. Genetic identification of a total of 41 eggs was attempted using the cytochrome c oxidase 1 (COI) gene. Due to the preservation technique used (formalin) and resulting DNA degradation, sequences from only 17 individuals could be recovered. In all cases, non-carp cyprinids were identified as the most likely species identity (usually a Notropis spp.). In previously published...
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This dataset has been archived; it has been superseded by version 4.0 (November 2022) which can be found at https://doi.org/10.5066/P9ZCMH8S. The dataset is composed of two tables containing environmental DNA (eDNA) data and site location information from round goby (Neogobius melanostomus) surveys conducted on parts of the Champlain Canal and upper Hudson River in New York during 2022. First posted May 5, 2022, ver. 1.0 Revised June 2022, ver. 2.0 Revised August 2022, ver. 3.0
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Environmental DNA (eDNA) water samples were collected at 15 tree islands containing wading bird breeding colonies (order Pelecaniformes) and 15 empty control islands in the central Everglades of Florida in spring of 2017 (April through June) and analyzed for the presence of eDNA from invasive Burmese pythons (Python bivittatus). The Burmese python is now established as a breeding population throughout south Florida, USA. Pythons can consume large quantities of prey and may be a particular threat to wading bird breeding colonies in the Everglades. To quantify python occupancy rates at tree islands where wading birds breed, we utilized environmental DNA (eDNA) analysis—a genetic tool which detects shed DNA in water...
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Abstract: Environmental DNA (eDNA) is DNA that has been released by an organism into its environment, such that the DNA can be found in air, water, or soil. In aquatic systems, eDNA has been shown to provide a sampling approach that is more sensitive for detecting target organisms faster, and less expensively than previous approaches. However, eDNA needs to be sampled in a manner that has been tested and found effective and, because single copies of target DNA are detected reliably, rigorous procedures must be designed to avoid sample contamination. Here we provide the details of a sampling protocol designed for detecting fish. This protocol, or very similar prototypes, has been used to collect data reported in...
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Data is a spreadsheet of the number of copies of Zebra Mussel DNA detected and the number of positive detections for Zebra Mussel DNA from water samples collected over 6 different substrates, at 4 depths in 2 lakes. The ash-free dry weight of the mussels at each of the sites is also included for each sampling location.
This dataset includes quantifications of bigheaded carp DNA found in water samples collected from the Wabash River along transects at 3 sites over time. The samples were collected at 18 equidistant points in a transect across the river at each site. Samples were collected in 2013 on May 29, 30, 31, June 5, 6, 7, 8, 9, 10, 11, 16, 17, 19, 20, 21, and 22. Quantitative polymerase chain reaction (qPCR) was performed to determine the DNA concentrations in two replicates using the bigheaded carp assay defined in Merkes and others 2014.
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Community composition data from multi-pass electrofishing surveys for assessing fish populations in headwater streams of the Adirondack Mountains, New York, USA. Each row represents one individual fish. This spreadsheet contains 11 columns. The first 7 columns describe the data (year collected, local site ID, stream name, latitude, longitude, USGS site number, and electrofishing pass number) and the remaining 4 columns describe each individual fish (common name, scientific name, total length (mm) and weight (g)). These data support the following publication: Baldigo, B.P., Sporn, L.A., George, S.D., and Ball, Jacob, 2017. Efficacy of environmental DNA to detect and quantify Brook Trout Salvelinus fontinalis, populations...
Data describe a designed environmental DNA (eDNA) survey for the detection of oyster mussel (Epioblasma capsaeformis) from field collected water samples. Parameters described include the limit of detection and limit of quantification of the assay; a list of freshwater mussel species tissue samples that were used to test specificity of the assay; and field collected water samples that were tested to detect the presence of oyster mussel DNA. Samples were collected from sites on the Clinch River, Tennessee from 2019 to 2021
This dataset includes quantifications of bigheaded carp DNA found in water samples collected from the Wabash River along transects at 3 sites over time. The samples were collected at 18 equidistant points in a transect across the river at each site. Samples were collected in 2013 on May 29, 30, 31, June 5, 6, 7, 8, 9, 10, 11, 16, 17, 19, 20, 21, and 22. Quantitative polymerase chain reaction (qPCR) was performed to determine the DNA concentrations in two replicates using the bigheaded carp assay defined in Merkes and others 2014.
This dataset includes quantifications of bigheaded carp DNA found in water samples collected from the Wabash River along transects at 3 sites over time. The samples were collected at 18 equidistant points in a transect across the river at each site. Samples were collected in 2013 on May 29, 30, 31, June 5, 6, 7, 8, 9, 10, 11, 16, 17, 19, 20, 21, and 22. Quantitative polymerase chain reaction (qPCR) was performed to determine the DNA concentrations in two replicates using the bigheaded carp assay defined in Merkes and others 2014.
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This dataset has been archived; it has been superseded by version 2.0 (June 2022) which can be found at https://doi.org/10.5066/P9ZCMH8S. The dataset is composed of two tables containing environmental DNA (eDNA) data and site location information from round goby (Neogobius melanostomus) surveys conducted on parts of the Champlain Canal and upper Hudson River in New York during spring 2022.


    map background search result map search result map Asian carp eggs cannot be distinguished from other cyprinid species on the basis of morphology alone: Supporting Data Wabash River, Indiana bigheaded carps environmental DNA: Data Community composition data for assessing fish populations in headwater streams of the Adirondack Mountains, New York, USA A Protocol for Collecting Environmental DNA Samples From Streams Environmental DNA mapping of Zebra Mussel populations: Data Burmese python environmental DNA data, and environmental covariates, collected from wading bird aggregations and control sites in the Greater Everglades Ecosystem, United States, in 2017 Wabash River, Indiana bigheaded carps environmental DNA: Data Wabash River, Indiana bigheaded carps environmental DNA: Data Wabash River, Indiana bigheaded carps environmental DNA: Data Environmental DNA data for Round Goby from the Champlain Canal (ver. 3.0, August 2022) Environmental DNA data for Round Goby from the Champlain Canal Environmental DNA data for Round Goby from the Champlain Canal (ver. 10.0, December 2023) Data on the detection of Epioblasma capsaeformis using a designed environmental DNA (eDNA) survey upstream and downstream of known populations in the Clinch River, Tennessee (2019 to 2021) A Protocol for Collecting Environmental DNA Samples From Streams Environmental DNA data for Round Goby from the Champlain Canal Environmental DNA data for Round Goby from the Champlain Canal (ver. 3.0, August 2022) Environmental DNA data for Round Goby from the Champlain Canal (ver. 10.0, December 2023) Burmese python environmental DNA data, and environmental covariates, collected from wading bird aggregations and control sites in the Greater Everglades Ecosystem, United States, in 2017 Asian carp eggs cannot be distinguished from other cyprinid species on the basis of morphology alone: Supporting Data Data on the detection of Epioblasma capsaeformis using a designed environmental DNA (eDNA) survey upstream and downstream of known populations in the Clinch River, Tennessee (2019 to 2021) Community composition data for assessing fish populations in headwater streams of the Adirondack Mountains, New York, USA Wabash River, Indiana bigheaded carps environmental DNA: Data Wabash River, Indiana bigheaded carps environmental DNA: Data Wabash River, Indiana bigheaded carps environmental DNA: Data Wabash River, Indiana bigheaded carps environmental DNA: Data Environmental DNA mapping of Zebra Mussel populations: Data