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With leadership and coordination provided by the Eastern Tallgrass Prairie and Big Rivers Landscape Conservation Cooperative, the U.S. Fish and Wildlife Service Landscape Conservation Cooperatives (LCCs), which collectively span the geographic extent of the Mississippi River Basin (MRB), have identified high nutrient runoff (a major contributor to Gulf of Mexico hypoxia), and declines in wildlife populations (especially grassland and riparian bird species), as major conservation challenges requiring collaborative action. This project focused on development and application of spatial decision support systems (DSSs), coupled with surveys of agricultural producers, to assist the LCC community and partner resource management...