Filters: Tags: freshwater mussel (X)
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The diagnosis of bacterial disease in freshwater unionid mussels has been hindered by a lack of baseline information regarding the microbial communities associated with these animals. In this study, we cultured and identified bacteria from the hemolymph of stable mussel populations from the upper Mississippi River basin and compared results to mussel populations associated with a mortality event in the Clinch River, VA and TN. Several bacterial genera were consistently identified across mussel species and locations, appearing to be part of the natural bacterial flora. One noteworthy isolate was identified from the Clinch River. Yokenella regensbergei was found with relatively high prevalence during the mortality...
Categories: Data;
Tags: Bacteria,
Clinch River, Virginia, USA,
Clinch River,Tennessee, USA,
Hemolymph,
USGS Science Data Catalog (SDC),
Pebble counts were performed to assess the bed surface grain size distribution at different habitats within a reach of the Big River near Washington State Park, Missouri. These data were collected in support of research assessing the hydraulic conditions of freshwater mussel habitat in the Big River. One component of this investigation was to examine the theoretical stability and instability of sediment particle sizes that are present within mussel habitats as well as within nearby reference habitats. These pebble counts furnished grain size data used for reference sediment stability predictions. Pebble counts were performed at seven different habitats in a study reach on the Big River following the methods of Wolman...
Here we provide public access to six DNA sequence alignments and details on all specimens utilized in Smith and Johnson (2020).
Categories: Data;
Tags: DNA,
USGS Science Data Catalog (SDC),
biota,
conservation,
freshwater mussel,
We generated multilocus DNA sequence data and traditional morphometric measurements to evaluate species boundaries in Fusconaia mitchelli. We sequenced three loci: the protein-coding mitochondrial DNA genes cytochrome oxidase subunit 1 and NADH dehydrogenase 1, and the nuclear ribosomal internal transcribed spacer 1. We also took three measurements, maximum length, maximum width, and maximum height, of mussel shells for morphometric analysis.
Here we provide public access to three DNA sequence alignments (COI, ND1, ITS1) and details on all specimens utilized in Keogh et al. (in review).
The Southeastern and Mid-Atlantic regions of the United States contain the greatest biodiversity of native freshwater mussels in the world, but they are highly imperiled due to habitat alteration and destruction, pollution and poor water quality, and the introduction of aquatic invasive species. Now they are at even greater risk from the stresses associated with climate change-induced sea level rise and its associated changes in salinity, water temperature, and stream flow. This project investigates the potential vulnerability and adaptation of a native freshwater mussel, the Tidewater Mucket, in coastal river systems and offer solutions for its conservation. Scientists have recently discovered a population of...
Categories: Project;
Types: Map Service,
OGC WFS Layer,
OGC WMS Layer,
OGC WMS Service;
Tags: 2019,
CASC,
Completed,
Data Visualization & Tools,
Data Visualization & Tools,
Data is included for three sets of laboratory experiments where various fish species were tested in several multi-week trials to determine their suitability in serving as parasitic hosts for larvae of the freshwater mussel named dwarf wedgemussel (Alasmidonta heterodon) from the Mid-Atlantic region and New England of the United States. The first set of experiments tested host suitability of multiple fish species, the second compared host suitability of groups of tessellated darters (Etheostoma olmstedi; a known host) from multiple locations, and the third tested host suitability of individual tessellated darters. Results are reported in numbers of juvenile mussels per fish (JPF; total juvenile mussels produced by...
Pebble counts were performed to assess the bed surface grain size distribution at five delineated mussel habitats in the Big River, Missouri. These data were collected in support of research assessing the mussel communities and characteristics of freshwater mussel habitats in the Big River (Albers and others, 2016; Roberts and others, 2016). Pebble counts were performed at the mussel habitats following the methods of Wolman (1954). Grain diameters of 99-100 particles were measured on the intermediate axis. The pebble count data are semi-continuous; grains smaller than 2 mm were classified as sand, and grains larger than 64 mm were classified as either cobble (65-256 mm) or boulder (greater than 256 mm) but measured...
This data release contains river bed surface grain size measurements, water-surface elevation measurements, and digital elevation models (DEMs) for select reaches of the Big River, Missouri. These data were collected in support of research assessing the hydraulic conditions of freshwater mussel habitat in the Big River. The individual metadata associated with each data type describe the process steps, data inputs and sources, and the locations where the data were collected.
The use of carbon dioxide to control aquatic invasive species (AIS) prompted an evaluation of its effects on native freshwater mussels that may reside in areas of infusion. We exposed juvenile mussels (~6-mo old) of two species, the fat mucket (Lampsilis siliquoidea) and the federally endangered Higgins’ Eye (L. higginsii) to CO2 concentrations proposed for AIS control (24,000 – 96,000 µatm pCO2 ) for 28 d followed by a 14-d recovery period and compared their survival, growth, and behavior. Additionally, tissue and shell condition and transcriptomic response of four genes were measured in L. siliquoidea. Survival was similar between species; the 14-d postexposure LC50 for L.higginsii was 61,000 µatm pCO2 (95% CL...
Chronic (28-d) toxicity of 4-nonylphenol (4-NP) to two commonly tested species of mussels: fatmucket (Lampsilis siliquoidea) and rainbow mussel (Villosa iris).
Data include burrowing behavior of juvenile freshwater mussels (Lampsilis siliquoidea); Villosa constricta; Megalonaias nervosa; Villosa iris; Lampsilis powellii; and Anodonta oregonensis) in three types of sediment [a sand/silt/clay mixture (49% sand) with a total organic carbon (TOC) content of about 3% obtained from northeastern Minnesota, a predominantly fine sand (82% sand) with a TOC content of about 1% obtained from southwest Missouri, and a coarse commercial sand with a diameter of < 0.5 mm (Granusil, #4030)].
Freshwater mussels of the order Unionida are among the most endangered animal groups globally, but the causes of population declines are often enigmatic with little known about the role of disease. In 2018, we collected wild adult pheasantshell (Actinonaias pectorosa) and mucket (Actinonaias ligamentina) during an epidemiologic survey investigating an ongoing mussel mass mortality event in the Clinch River, USA. Histopathology and transmission electron microscopy showed a novel microsporidian parasite primarily infecting the ovary of pheasantshell. Sequencing of the small subunit rRNA gene produced a 1333 bp sequence with greatest similarity to Pseudonosema cristatellae (AF484694.1; 86.36%; e-value = 0), a microsporidium...
Categories: Data;
Tags: Actinonaias pectorosa,
Clinch River, Virginia and Tennessee, USA,
Microsporidium clinchi n. sp.,
PCR,
TEM,
Water-surface elevation measurements were performed for a range of flow events up to bankfull discharge within four reaches of the Big River, Missouri. The study reaches are Site W (STW), Rockford Beach (RFB), Phelps Bend (PHB), and Washington State Park (WSP). The water-surface elevation measurements were collected to provide hydraulic model calibration data in support of research assessing the hydraulic conditions of freshwater mussel habitat in the Big River. Data were collected either manually or by boat in the study reaches using a Global Navigation Satellite System (GNSS) receiver. Calibration data also included water-surface elevation data from bathymetry and terrestrial lidar surveys. Models were calibrated...
Data is included for two types of field surveys conducted for freshwater mussels in the mainstem of the middle and upper Delaware River in the Mid-Atlantic region of the United States from 2000-2002. Timed search (qualitative) surveys were conducted during 2000-2001 from a point at the confluence of the East and West Branches of the Delaware River near Hancock, NY continuously downstream to a point at the mouth of the Paulins Kill River near Columbia, NJ. In this qualitative survey, mussel species and counts were collected in the field catch-per-unit-effort (CPUE) data was determined for all mussel species within each of 1,095 consecutive stream sections ~200 m in length. Subsequent quantitative surveys were conducted...
Categories: Data;
Tags: Aquatic Biology,
Delaware River,
Delaware Water Gap National Recreation Area,
Ecology,
New Jersey,
The data consists of targeted metabolite analysis (using quadrupole time-of-flight mass spectroscopy;UHPLC-QTOF-MS) of hemolymph from two freshwater mussel species (Lampsilis cardium and L. siliquoidea) from three streams in Indiana. Concentrations of metabolites were modeled by species, stream and sex to determine the influence of each parameter.
The role of disease in freshwater mussel declines has been largely ignored due to the lack of appropriate diagnostic tools and metabolomic markers of stress. Mussels in this study were either fed a prepared diet or unfed and their condition was assessed with the observed changes in fatty acid content of their tissue. This dataset contains quantitative fatty acid data from nonlethal (biopsy) sampling of foot tissue. These data may provide biomarkers indicative of freshwater mussel’s metabolic status.
This dataset includes information from multiple taxa collected from four main reaches in the Upper Mississippi River Basin, including La Grange reach of Illinois River, Pool 2 of Mississippi River, Pool 19 of Mississippi River, and the St. Croix River. Taxa include hydropsychid caddisflies, chironomids, hexagenia mayflies, threeridge mussels, mapleleaf mussels, Wabash pigtoe mussels, bigmouth buffalo, gizzard shad, and bluegill. Seston samples were also collected, in addition to water samples for identifying and enumerating phytoplankton species. Fatty acids, lipids, and stable isotopes were quantified for the aforementioned taxa. Water quality data were recorded at the time of sample collection and discharge data...
This dataset contains digital elevation models (DEMs) for four reaches of the Big River in Missouri. The study reaches are as follows: Site W (STW), Rockford Beach (RFB), Phelps Bend (PHB), and Washington State Park (WSP). The reach DEMs were created in support of research assessing the hydraulic conditions of freshwater mussel habitat in the Big River. Channel and bank elevations were generated from data collected by USGS staff from the Columbia Environmental Research Center between February 2019 and January 2020 using single-beam echosounder surveys, manual Global Navigation Satellite System (GNSS) topographic surveys, and terrestrial light detection and ranging (lidar) surveys. Elevations for areas beyond the...
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