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We provide genetic and distribution data for the Louisiana Pigtoe, Pleurobema riddellii, to guide decisions related to federal protection for the species under the U.S. Endangered Species Act. Our final dataset includes 125 COI sequences generated as part of our study representing P. riddellii from eight North American river basins. This includes the COI sequence we generated from a museum specimen collected from the Trinity River in the late 1900s, which is the first genetic confirmation of P. riddellii in the Trinity River basin. Also included are sequences from specimens initially identified as Fusconaia cerina (n=3) Fusconaia flava (n=1), Fusconaia sp. (n=5), Pleurobema sintoxia (n=10), and Pustulosa pustulosa...
The data support a study that describes the development and validation of a primer and probe based quantitative PCR (qPCR) assay for use with environmental DNA to detect Northwest salamander (Ambystoma gracile), a species endemic to the temperate Pacific coastal region of North America. The metadata includes qPCR quantification cycle (Cq) values from testing the A. gracile assay on DNA extracted from tissue samples derived from several A. gracile and closely related species and Cq values from testing the assay on environmental DNA (eDNA ) samples collected from two lakes, one containing A. gracile and one without the species.
Categories: Data;
Tags: Aquatic Biology,
Genetics,
Pacific Northwest,
USGS Science Data Catalog (SDC),
amphibians,
We investigated fine-scale genetic patterns of the federally threatened Eastern Massasauga Rattlesnake (Sistrurus catenatus) on a relatively undisturbed island in northern Michigan, USA. This species often persists in habitat islands throughout much of its distribution due to extensive habitat loss and distance-limited dispersal. These data are from 102 individual Eastern Massasauga Rattlesnakes sampled at Bois Blanc Island, Michigan and genotyped at 15 microsatellite loci. Samples were collected as part of a study to examine functional connectivity for the Eastern Massasauga. We found that the entire island population exhibited weak genetic structuring with spatially segregated variation in effective migration...
The data release details the samples, methods, and raw data used to generate high-quality genome assemblies for greater sage-grouse (Centrocercus urophasianus), white-tailed ptarmigan (Lagopus leucura), and trumpeter swan (Cygnus buccinator). The raw data have been deposited in the Sequence Read Archive (SRA) of the National Center for Biotechnology Information (NCBI), the authoritative repository for public biological sequence data, and are not included in this data release. Instead, the accessions that link to those data via the NCBI portal (www.ncbi.nlm.nih.gov) are provided herein. The release consists of a single file, sample.metadata.txt, which maps NCBI accessions to the samples sequenced and the different...
Dataset containing information for white-tailed deer samples from Ohio, Pennsylvania, Maryland, Virginia and New York, genotyped for 11 microsatellites markers. Marker OvirQ should not be used as it presents alleles inconsistent with reported pattern, with some alleles separated by only 1 base pair and inconsistent between runs. Projected coordinates representing sampling location are in a user-defined CRS, similar to USA Contiguous Albers Equal Area Conic: "+proj=aea +lat_1=29.3 +lat_2=45.3 +lat_0=23 +lon_0=-96 +x_0=0 +y_0=0 +ellps=GRS80 +towgs84=0,0,0,0,0,0,0 +units=m +no_defs".
We analyzed the single nucleotide polymorphisms (SNPs) present in the genomes of moose representing 3 subspecies in the contiguous United States. Blood samples were collected opportunistically from collaborators during field efforts or were supplied to our lab from collaborators' archives, and represented moose sampling occurring between 2009-2017. DNA was extracted, sequenced using next generation sequencing, and SNPs analyzed using the genetics programs Structure and Tess3, and by performing basic population statistics. These analyses were used to determine the population structure of moose at the subspecies level.
These are genetic data collected from replicated samples of 21 Enteroctopus (E. dofleini or a cryptic lineage) in Prince William Sound Alaska to evaluate tissue type, DNA extraction method, and time until analyses are completed on data reliability. Data collected from all samples include two microsatellites identified as possible lineage indicators, and nine microsatellite loci previously identified as polymorphic in both lineages. DNA sequence data from 528 bp of the octopine dehydrogenase (OCDE) gene were also collected.
Categories: Data;
Tags: Amino Acid Oxidoreductases,
Animals/Invertebrates,
Bald Head Chris Island,
Biodiversity,
Biodiversity,
The data support a study that surveyed the spatial distribution of Oncorhynchus mykiss and Cottus aleuticus eDNA in coastal streams of Big Sur, California, 2021-2022 following post-fire debris flows. The metadata represent qPCR quantification cycle (Cq) values for O. mykiss and C. aleuticus assays performed on water samples collected during June and July of 2021 and 2022 from the following streams: Big Creek, Mill Creek, Prewitt Creek, and Willow Creek. The metadata also includes the distance (meters) of each eDNA sample site from the stream mouth, volume of water (Liters) collected for eDNA analysis at each site, and the Y-intercept, slope, and R-squared value for each assay run.
This project used species distribution modeling, population genetics, and geospatial analysis of historical vs. modern vertebrate populations to identify climate change refugia and population connectivity across the Sierra Nevada. It is hypothesized that climate change refugia will increase persistence and stability of populations and, as a result, maintain higher genetic diversity. This work helps managers assess the need to include connectivity and refugia in climate change adaptation strategies. Results help Sierra Nevada land managers allocate limited resources, aid future scenario assessment at landscape scales, and develop a performance measure for assessing resilience.
Categories: Data,
Project;
Tags: 2011,
2013,
CA,
California Landscape Conservation Cooperative,
Conservation Design,
Latitudes and longitudes for 45 sites samples for flathead catfish population genetic analyses.
We tested the utility of a protocol using genetic markers that previously proved successful to identify the sex of Vespertilionid bats on tissues collected from live bats and carcasses of varying age from the Hawaiian hoary bat (Lasiurus semotus). This molecular method is based on genes unique to X and Y chromosomes in mammals and previously was used successfully on North American hoary bats (L. cinereus). Using genotyping, we determined the sex of 123 of 125 Hawaiian hoary bat tissue samples from carcasses. This dataset includes all 73 samples from the original data release, plus 58 additional samples. NOTE: While previous versions are available from the author, all the records in previous versions can be found...
Categories: Data,
Data Release - Revised;
Tags: Hawaii,
Hawaiian Islands,
Hawaiian hoary bat,
Maui,
Oahu,
The Grand Calumet River flows through northwestern Indiana and was designated an EPA Area of Concern (AOC) in 1987. There are multiple legacy pollutants in the river including but not limited to heavy metals and PCBs. To investigate potential causes and patterns of eutrophication in the river, water samples were collected for 16S rRNA microbial and eukaryotic 18S rRNA amplicon sequencing during April - September 2021. This data release describes the resulting sequencing data.
Categories: Data;
Tags: Aquatic Biology,
DNA Sequencing,
Environmental Health,
Environmental Health,
Eukaryotic 18S metabarcoding,
These data were compiled to investigate the evolutionary history of Hilaria jamesii, Hilaria mutica, and Hilaria rigida. The data release consists of two tab delimited text files that may be used to infer population structure (viva_structure.stru) or relationships among sampling localities (viva.phylip). Files record genetic variation on an individual (.stru) or sampling locality (.phylip) level. These files may be opened and edited in a text editor program, such as Notepad ++ (PC) or BBEdit (Mac). The .phylip file can be uploaded to phyML or SVDQuartets to generate a tree-based visualization of relationships ( http://www.atgc-montpellier.fr/phyml/ or https://paup.phylosolutions.com, repectively). The .stru file...
These data include metadata and associated data files associated with the manuscript, "Economical Environmental Sampler Designs for Detecting Airborne Spread of Fungi Responsible for Rapid ʽŌhiʽa Death." These data include a total of 8 datasets used for both controlled and field studies evaluating the use of Active (with battery operated fan) and Passive (dependent on wind) USGS Environmental Samplers on Hawaii Island between 2016-2018. Samplers were operated under controlled laboratory and field conditions with a commercial sampler (Rotorod® Model 20) to compare efficacy in capturing synthetic polyethylene spheres (12 - 160 µm in diameter) and also Xyleborus spp. boring dust (frass) known to contain the fungi responsible...
High-throughput methods for identification of pollinator taxa are desirable to improve our understanding of pollinator distributions, population trends, and ecology. Genetic sequencing of taxonomically informative 'barcode' loci is one high-throughput strategy, which can be applied to individual specimens using Sanger technology and to complex mixtures using metabarcoding technology. This study generated Sanger sequencing data from morphologically identified specimens and metabarcoding data derived from pooled tissues. This data release consists of several files: 1. sample.metadata.txt, which contains sampling metadata and identifiers linking to sequence data that has been deposited in the Sequence Read Archive...
The Coastal Cactus Wren is a species of high conservation concern in southern California. The goal of this project was to obtain taxonomic resolution of subspecies boundaries between coastal and desert populations of Cactus Wrens. We collected genetic samples from Cactus Wrens at California desert sites and combined this dataset with previously collected samples from coastal populations to resolve the distribution of subspecies and to determine distinct genetic lineages, gene flow, genetic diversity and effective population size of desert populations. Understanding these relationships will assist managers with development and implementation of a conservation and management strategy that could aim to avoid state...
Categories: Data;
Tags: California,
DNA sequencing,
Ecology,
Genetics,
USGS Science Data Catalog (SDC),
Here we provide the molecular datasets and metadata associated with specimens used in the investigation of species boundaries and phylogeographic structure of two freshwater mussels, one common (Pleurobema sintoxia) and one being considered for protection under the Endangered Species Act (Pleurobema rubrum).
Categories: Data;
Tags: Eastern United States,
Genetics,
USGS Science Data Catalog (SDC),
biota,
conservation genetics,
Delivering adequate water supplies to support expanding human enterprise while maintaining the necessary flow regimes to support desired riparian ecosystems and formally protected wildlife species that depend upon them is increasingly difficult in the arid western United States. Many riparian systems have undergone dramatic alteration over the last 50 - 100 years, exacerbating the conflicts between resource use and biodiversity protection. One of the most visible changes that is in part due to altered flow regimes is the establishment of invasive plant species in riparian ecosystems. The highest priority invasive riparian plant is the Eurasian tree/shrub, tamarisk (or saltcedar, Tamarix spp.) the third most abundant...
Categories: Data,
Project;
Types: Map Service,
OGC WFS Layer,
OGC WMS Layer,
OGC WMS Service;
Tags: 2012,
AZ-01,
AZ-02,
AZ-03,
AZ-04,
Collection of microsatellite genetic data from multiple projects involving southern mule deer (Odocoileus hemionus fuliginatus) in San Diego County, California. Samples were collected 2005-2007, 2010, 2012-2013, 2015, and 2018-2020. These data support the following publication: Mitelberg, A., Smith, J.G., and Vandergast, A.G., 2019, DNA Fingerprinting of Southern mule deer (Odocoileus hemionus fuliginatus) in north San Diego County, California (2018–19): U.S. Geological Survey Open-File Report 2019–1138, 25 p., https://doi.org/10.3133/ofr20191138.
Categories: Data;
Tags: DNA sequencing,
Ecology,
Genetics,
San Diego,
USGS Science Data Catalog (SDC),
The dataset consists of genotypes (diploid base calls at variant sites) at 3,601 anonymous sites of the Arizona Toad (Anaxyrus microscaphus) nuclear genome. The genotyped samples are representative of the range of the species and its major population units, and the genotyped loci have a high degree of completeness. This data release consists of several files: 1. sample.metadata.txt, which contains sampling metadata and identifiers linking to sequence data that has been deposited in the Sequence Read Archive of the National Center for Biotechnology Information (NCBI). This database is authoritative and comprehensive for sharing high-throughput sequence data produced with public funds. All NCBI-derived accessions...
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