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The 53 papers in this proceedings include a section celebrating the 25-year anniversary of the Shrub Sciences Laboratory (4 papers), three sections devoted to themes, genetics, and biodiversity (12 papers), disturbance ecology and biodiversity (14 papers), ecophysiology (13 papers), community ecology (9 papers), and field trip section (1 paper). The anniversary session papers emphasized the productivity and history of the Shrub Sciences Laboratory, 100 years of genetics, plant materials development for wildland shrub ecosystems, and current challenges in management and research in wildland shrub ecosystems. The papers in each of the thematic science sessions were centered on wildland shrub ecosystems. The field...
Categories: Publication;
Types: Citation;
Tags: biodiversity,
community ecology,
disturbance,
ecophysiology,
genetics,
This dataset provides spatial predictions of clustering and the genotype association index for the Mojave genotype in local species-environment relationships of Desert Tortoises (Gopherus agassizi and Gopherus morafkaii) for individuals in the subregion encompassing the genetic sampling locations used by Edwards et al. (2015). This region offered an opportunity to explore habitat selection across the ecotone between the Mojave and Sonoran deserts and the secondary contact zone between G. agassizii and G. morafkai, and is referred to as the focal study area. The raster layers contained here accompany the manuscript Inman et al. 2019 and were used to identify multivariate clusters and map them back to geographic space....
To understand the genetic structure of six rare plant species (Acanthomintha ilicifolia, Baccharis vanessae, Chloropyron maritimum ssp. maritimus, Deinandra conjugens, Dicranostegia orcuttiana, Monardella viminea), we obtained samples from known occurrences in San Diego County, prepared and sequenced ddRAD genomic libraries, and developed and analyzed a panel of genetic markers to assess population diversity and divergence. We provide this SNP genotype dataset in Structure format, produced in Stacks v2.0b8, for future monitoring efforts and population genetic analysis. These data support the following publication: Milano, E.R., and Vandergast, A.G., 2018, Population genomic surveys for six rare plant species in...
The rusty-patched bumble bee (Bombus affinis) is a listed species under the Endangered Species Act of the United States. While understood to be a highly generalist forager, little is known on the role that limited floral resources or shifting floral community composition could have played in B. affinis decline. Determining which floral species provide suitable B. affinis forage could assist conservation efforts where B. affinis persists, identify floral species for restoration efforts, or highlight regions to search for cryptic populations.
Conversion and fragmentation of wildlife habitat often leads to smaller and isolated populations and can reduce a species’ ability to disperse across the landscape. As a consequence, genetic drift can quickly lower genetic variation and increase vulnerability to extirpation. For species of conservation concern, quantification of population size and connectivity can clarify the influence of genetic drift in local populations and provides important information for conservation management and recovery strategies. Here, we used genome-wide single nucleotide polymorphism (SNP) data and capture-mark-recapture methods to evaluate the population structure, genetic diversity and abundance of seven focal sites of the endangered...
To evaluate the ability of precipitation-based environmental DNA (eDNA) sample collection and mitochondrial 12S metabarcoding sequencing to reconstruct well-studied fish communities in lakes and rivers. Specific objectives were to 1) determine correlations between eDNA species detections and known community composition based on traditional field sampling, 2) compare efficiency of eDNA to detect fish biodiversity among systems with variable morphologies and trophic states, and 3) determine if species habitat preferences predicts eDNA detection. Fish community composition was estimated for seven lakes and two MIssissippi River navigation pools using sequence data from the mitochonrial 12S gene amplified from 10 to...
These datasets were developed to represent the genetic diversity, population structure, and geographic distribution of Plantago ovata in the Mojave Desert. This data release consists of two tab-delimited text files representing the genetic diversity and structure of Plantago ovata (.genepop and .vcf), and two raster spatial datasets (GeoTIFF) reflecting predicted habitat for the species within the Mojave Desert. The genetic datasets record genetic variation at an individual level, with the file structures varying based on the programs within which the files are intended to be edited. Each file contains 13,111 SNPs genotyped in 748 individuals. The genepop file can be viewed in GENEPOP software (Rousset 2008) or...
Types: Map Service,
OGC WFS Layer,
OGC WMS Layer,
OGC WMS Service;
Tags: Arizona,
Botany,
California,
Genetics,
Mojave,
We developed and validated conventional and quantitative real-time PCR assays for the detection of DNA from the myxozoan parasite Tetracapsuloides bryosalmonae, the causative agent of proliferative kidney disease in fish. Assays were tested on fish tissue and on field-collected water samples to assess diagnostic and environmental DNA capabilities. The specificity, sensitivity, and broad applicability of the present assays surpass previous methods for detecting T. bryosalmonae DNA from fish tissue and water samples.
This U.S. Geological Survey (USGS) data release provides discretely measured cyanotoxin, chlorophyll-a, and cyanobacterial toxin genetic data for samples collected from eleven large river sites throughout the United States, from June through October 2018. Discrete water-quality samples were analyzed for cyanotoxins (anatoxin, cylindrospermopsin, microcystin, and saxitoxin), chlorophyll-a, and cyanobacterial toxin genetics as part of a National Water-Quality Assessment Project pilot study to describe cyanobacteria and cyanotoxin occurrence in the Nation's large rivers.
Categories: Data;
Tags: Anatoxin,
Aquatic Biology,
California,
Chattahoochee River near Whitesburg, GA,
Chlorophyll-a,
The data support a study that surveyed the spatial and temporal distribution of salmon eDNA in Seattle urban creeks, Washington, 2018 - 2020. The metadata represent qPCR quantification cycle (Cq) values for Chinook salmon, coho salmon, and coastal cutthroat trout assays performed on water samples collected on specific days at specific sites on Thornton Creek, Taylor Creek, and Mapes Creek, which are tributaries of Lake Washington within Seattle city limits. The metadata also includes latitude and longitude for each site and Y-intercept and slope for each assay run.
Monitoring change in genetic diversity in wildlife populations across multiple scales could facilitate prioritization of conservation efforts. We used microsatellite genotypes from 7,080 previously collected genetic samples from across the greater sage-grouse (Centrocercus urophasianus) range to develop a modelling framework for estimating genetic diversity within a recently developed hierarchically nested monitoring framework (clusters). The majority of these genetic samples (n=6560) were used in previous research (Oyler-McCance et al. 2014; Cross et. al 2018; Row et. al. 2018). Genetic diversity values associated with clusters across multiple scales could facilitate the identification of areas with low genetic...
Reported here are the metabarcoding read numbers and biomass data assessed from a fish community in a southern Florida pond in Pinecrest Gardens after invasive species eradication and native restocking in 2017 and 2018 in linear regression models. This dataframe contains the number of fish of each species identified, their measured lengths estimated population surface area, and the number of metabarcoding reads (with SD and COV) for each species observed from the sampling date.
Categories: Data;
Tags: Ecology,
Genetics,
Genetics,
Southern Florida,
USGS Science Data Catalog (SDC),
The size and sex of each of the Burmese pythons swabbed in this study for the SFD-causing (snake fungal disease) Ophidiomyces ophiodiicola pathogen is given along with the real time PCR swab result.
Categories: Data;
Tags: Ecology,
Florida,
Genetics,
Southern Florida,
USGS Science Data Catalog (SDC),
Here we provide public access to six DNA sequence alignments and details on all specimens utilized in Smith and Johnson (2020).
Categories: Data;
Tags: DNA,
USGS Science Data Catalog (SDC),
biota,
conservation,
freshwater mussel,
Longstanding taxonomic uncertainties have limited conservation efforts for species currently assigned to the freshwater mussel genus Alasmidonta. Here, we present mitochondrial and nuclear DNA sequence data needed to assess the genus- and species-level taxonomy of Alasmidonta. These molecular data allowed us to test whether cryptic diversity exists within Alasmidonta and whether A. triangulata and A. arcula are distinct species. Details associated with specimens and DNA sequence data are provided here to provide a foundation for future research on Alasmidonta and give conservation agencies greater confidence in the findings of our work.
Categories: Data;
Tags: DNA sequencing,
Genetics,
USGS Science Data Catalog (SDC),
biota,
conservation genetics,
We analyzed the single nucleotide polymorphisms (SNPs) present in the genomes of moose representing 3 subspecies in the contiguous United States. Blood samples were collected opportunistically from collaborators during field efforts or were supplied to our lab from collaborators' archives, and represented moose sampling occurring between 2009-2017. DNA was extracted, sequenced using next generation sequencing, and SNPs analyzed using the genetics programs Structure and Tess3, and by performing basic population statistics. These analyses were used to determine the population structure of moose at the subspecies level.
These are genetic data collected from replicated samples of 21 Enteroctopus (E. dofleini or a cryptic lineage) in Prince William Sound Alaska to evaluate tissue type, DNA extraction method, and time until analyses are completed on data reliability. Data collected from all samples include two microsatellites identified as possible lineage indicators, and nine microsatellite loci previously identified as polymorphic in both lineages. DNA sequence data from 528 bp of the octopine dehydrogenase (OCDE) gene were also collected.
Categories: Data;
Tags: Amino Acid Oxidoreductases,
Animals/Invertebrates,
Bald Head Chris Island,
Biodiversity,
Biodiversity,
The data support a study that surveyed the spatial distribution of Oncorhynchus mykiss and Cottus aleuticus eDNA in coastal streams of Big Sur, California, 2021-2022 following post-fire debris flows. The metadata represent qPCR quantification cycle (Cq) values for O. mykiss and C. aleuticus assays performed on water samples collected during June and July of 2021 and 2022 from the following streams: Big Creek, Mill Creek, Prewitt Creek, and Willow Creek. The metadata also includes the distance (meters) of each eDNA sample site from the stream mouth, volume of water (Liters) collected for eDNA analysis at each site, and the Y-intercept, slope, and R-squared value for each assay run.
The Utah sucker (Catostomus ardens) is endemic to the Bonneville Basin and the upper Snake River drainage in western North America, and is thought to hybridize with the federally endangered June sucker (Chasmistes liorus mictus) in Utah Lake (Bonneville Basin). Here we describe the discovery of a major subdivision in Utah suckers (4.5% mitochondrial sequence divergence) between the ancient Snake River drainage and the Bonneville Basin. This boundary has not previously been recognized in Utah suckers based on morphologic variation, but has been recently described in two endemic cyprinids in the region. Populations in valleys east of the Wasatch Mountains in Utah clustered with the Snake River populations, suggesting...
Categories: Publication;
Types: Citation,
Journal Citation;
Tags: Cypriniformes,
Cypriniformes: genetics,
DNA,
Molecular Ecology,
Utah,
These data consist of microsatellite genotype scores for all samples of Santa Ana sucker (Catostomus santaanae) used in the study. Scores represent the allele calls for each microsatellite locus (i.e. DNA fragment length containing the microsatellite repeats), with each locus containing two scores representing the two allele copies detected. Included are five tables: Full dataset (includes genotypes from all samples), Santa Clara River samples only (includes genotypes only from samples collected in the Santa Clara River), Convert File format key (explains the data file format), Population identifiers (translates the numerical population identifiers to actual collecting sites), CASA sampling points (one coordinate...
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