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The quantity of water that should be retained in streams and rivers for the benefit of fish during periods of water scarcity is a question of considerable interest to river managers and biologists. Although instream flow methodologies have existed since the 1970s, no single method has been widely accepted for use on large warm-water rivers because of their high species richness and generalized fish habitat use patterns. In this paper, we present an approach similar to instream flow incremental methodology, but which uses two-dimensional flow models and biomass estimates derived from multiple sites on two Colorado rivers for predicting the effect of discharge on adult standing stocks of two native fish species. Suitability...