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We investigated the behavioral response of female Pacific lamprey to three odors and to differences in water flow. Adult lamprey were challenged with: 1) washings from conspecifics, 2) with washings from adult males, and 3) with an artificial odor that mimicked a component of a natural lamprey pheromone. In addition, we conducted parallel tests to check for rheotaxis. The fish were tested in two Y-mazes where they were exposed to an odor and a control (no odor). The fish were pit-tagged and monitored with four antennas. During overnight tests we counted the number of entries into each arm of the maze and the amount of time spent in each arm.